Himachal Journal of Agricultural Research

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Development and Validation of Gene-Derived Cleaved Amplified Polymorphic Sequences (CAPS) Marker for Blast Resistance Gene Pi54


Affiliations
1 Department of Agricultural Biotechnology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, India
2 School of Biotechnology Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu Chatha, Jammu-180 009 (Jammu & Kashmir), India
 

Rice blast is one of the world’s most damaging diseases attacking the rice crop. Functional or gene-based markers derived from the polymorphic sites within the nucleotide sequences of cloned R-genes are the potent tools for precise and speedy selection of resistance genes in marker-assisted breeding programmes. The Pi54 gene identified from a broad spectrum genotype Tetep is known to exhibit resistance to predominant races of pathogen in India thus making it a potential resistance source for breeding blast resistant varieties. The Pi54 gene has been cloned thus offering a scope for the development of gene-derived markers for this useful gene by using the sequence polymorphisms between the resistant and susceptible haplotypes/alleles of the gene. The development of a new gene-based Cleaved Amplified Polymorphic Sequences (CAPS) marker for Pi54 gene and its utility in the marker-assisted selection of this broad-spectrum resistance gene has been reported. The developed marker has been shown to be perfectly linked with Pi54 and works well for crosses where the previously known gene-based marker Pi54MAS fails to reveal polymorphism between the resistant and susceptible genotypes.

Keywords

Blast Resistance, CAPS, Gene-Derived Markers, Pi54, Rice.
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  • Development and Validation of Gene-Derived Cleaved Amplified Polymorphic Sequences (CAPS) Marker for Blast Resistance Gene Pi54

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Authors

T. D. Pote
Department of Agricultural Biotechnology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, India
K. D. Sharma
Department of Agricultural Biotechnology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, India
R. K. Salgotra
School of Biotechnology Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu Chatha, Jammu-180 009 (Jammu & Kashmir), India
R. Rathour
Department of Agricultural Biotechnology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, India

Abstract


Rice blast is one of the world’s most damaging diseases attacking the rice crop. Functional or gene-based markers derived from the polymorphic sites within the nucleotide sequences of cloned R-genes are the potent tools for precise and speedy selection of resistance genes in marker-assisted breeding programmes. The Pi54 gene identified from a broad spectrum genotype Tetep is known to exhibit resistance to predominant races of pathogen in India thus making it a potential resistance source for breeding blast resistant varieties. The Pi54 gene has been cloned thus offering a scope for the development of gene-derived markers for this useful gene by using the sequence polymorphisms between the resistant and susceptible haplotypes/alleles of the gene. The development of a new gene-based Cleaved Amplified Polymorphic Sequences (CAPS) marker for Pi54 gene and its utility in the marker-assisted selection of this broad-spectrum resistance gene has been reported. The developed marker has been shown to be perfectly linked with Pi54 and works well for crosses where the previously known gene-based marker Pi54MAS fails to reveal polymorphism between the resistant and susceptible genotypes.

Keywords


Blast Resistance, CAPS, Gene-Derived Markers, Pi54, Rice.

References