Himachal Journal of Agricultural Research

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Studies on Induced Chlorophyll Mutants in Black Gram (Vigna mungo L. Hepper)


Affiliations
1 Department of Genetics and Plant Breeding, CSK Himachal Pradesh Krishi Vishvavidyalaya, 176 062, India
 

The investigation was undertaken to score and identify the chlorophyll mutants in M generation of two 2 genotypes of black gram viz., HM-1 and PLP-93 during Kharif 2019. M generation was raised in Kharif 2018 1 from the seeds of both the genotypes treated with gamma-rays (100, 200, 300 and 400 Gy), EMS (0.1, 0.2, 0.3 and 0.44%) and their possible combinations. Five types of chlorophyll mutants were detected. Most frequent chlorophyll mutant was chlorina (31) followed by albino (15), xantha (12), variegated (7) and striata (6). The frequency of mutants showed gradual increase with increase in dose/concentration of mutagens in both gamma rays and EMS, while the combination treatments showed an irregular trend with increasing dose in both the genotypes of black gram. Combined treatments produced more mutant frequency than gamma-irradiation and EMS treatments for both genotypes of black gram. Genotype HM-1 was observed more responsive to mutation changes than the genotype PLP-93.

Keywords

Chlorophyll Mutants, EMS, Gamma-Rays, Mutant Frequency, Black Gram.
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  • Studies on Induced Chlorophyll Mutants in Black Gram (Vigna mungo L. Hepper)

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Authors

Navdeep Kaur
Department of Genetics and Plant Breeding, CSK Himachal Pradesh Krishi Vishvavidyalaya, 176 062, India
R. K. Mittal
Department of Genetics and Plant Breeding, CSK Himachal Pradesh Krishi Vishvavidyalaya, 176 062, India
V. K. Sood
Department of Genetics and Plant Breeding, CSK Himachal Pradesh Krishi Vishvavidyalaya, 176 062, India
Alka Soharu
Department of Genetics and Plant Breeding, CSK Himachal Pradesh Krishi Vishvavidyalaya, 176 062, India

Abstract


The investigation was undertaken to score and identify the chlorophyll mutants in M generation of two 2 genotypes of black gram viz., HM-1 and PLP-93 during Kharif 2019. M generation was raised in Kharif 2018 1 from the seeds of both the genotypes treated with gamma-rays (100, 200, 300 and 400 Gy), EMS (0.1, 0.2, 0.3 and 0.44%) and their possible combinations. Five types of chlorophyll mutants were detected. Most frequent chlorophyll mutant was chlorina (31) followed by albino (15), xantha (12), variegated (7) and striata (6). The frequency of mutants showed gradual increase with increase in dose/concentration of mutagens in both gamma rays and EMS, while the combination treatments showed an irregular trend with increasing dose in both the genotypes of black gram. Combined treatments produced more mutant frequency than gamma-irradiation and EMS treatments for both genotypes of black gram. Genotype HM-1 was observed more responsive to mutation changes than the genotype PLP-93.

Keywords


Chlorophyll Mutants, EMS, Gamma-Rays, Mutant Frequency, Black Gram.

References