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Sharma, Kamal Dev
- Effect of Heat Stress on Expression of Glucose-6-Phosphate/Phosphate Translocators in Chickpea Leaves
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Authors
Affiliations
1 Department of Agricultural Biotechnology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, IN
2 Department of Plant Pathology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, IN
1 Department of Agricultural Biotechnology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, IN
2 Department of Plant Pathology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, IN
Source
Himachal Journal of Agricultural Research, Vol 46, No 2 (2020), Pagination: 119-129Abstract
Sugars, besides source of energy, also provide tolerance and acclimation to plants under abiotic stresses. In plants, sugars are transported via specialized protein molecules called as sugar transporters. One of the most ubiquitous soluble sugars in plants, glucose-6-phosphate, is transported from cytosol into the chloroplast by glucose-6-phosphate/phosphate translocators (GPTs). Search of chickpea sequences revealed existence of two phylogentically diverse GPT genes in chickpea named as GPT1 and GPT2. The gene GPT1 (coding region: 1200 bases, 41.58% GC content) is present on chromosome 5 whereas GPT2 (coding region: 1164 bases, 38.74% GC content) on chromosome 1. Of these two, GPT1 was not active in chickpea leaves whereas GPT2, under heat stress, over-expressed vis-à-vis control in leaves of heat-tolerant ICC 15614 and down-regulated in heatsusceptible ICC 10685 suggesting that GPT2 is associated with heat tolerance in chickpea. The GPT2 can be a potential candidate gene for heat-tolerance in chickpea.Keywords
Chickpea, Cicer arietinum, Glucose-6-phosphate Transporter, Gene Expression, High Temperature, Heat Stress.Full Text
References
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- Ascochyta rabiei Infections Modify Expression of Chickpea Invertase Genes Differentially in Contrasting Genotypes
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Authors
Affiliations
1 Department of Agricultural Biotechnology, College of Agriculture CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176062, IN
1 Department of Agricultural Biotechnology, College of Agriculture CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176062, IN
Source
Himachal Journal of Agricultural Research, Vol 48, No 01 (2022), Pagination: 1-7Abstract
Sucrose is the main form of assimilated carbon and energy source in plants. Sucrose in sink tissues is hydrolyzed by invertases to glucose and fructose that act as major carbon molecules for plant metabolism. Initial studies in some crops suggested role of invertases in plant disease resistance however, no information on these genes is available for chickpea biotic stresses. To identify the role of invertases in resistance/susceptibility to ascochyta blight (causal organism: Ascochyta rabiei) in chickpea, expression of six invertase genes (two cell wall invertases, one vacuolar invertase and three alkaline/neutral invertases) was evaluated in A. rabiei infected susceptible (GPF2) and resistant (HC1) genotypes of chickpea. Of these six gene, only one overexpressed in susceptible GPF2 whereas in resistant HC1 five genes overexpressed. The study suggested that down regulation of invertase genes was associated with susceptibility of chickpea to A. rabiei whereas over expression was associated with resistance.Keywords
Ascochyta blight, Cicer arietinum, cell wall invertase, vacuolar invertase, alkaline/neutral invertase, gene expression, Ascochyta rabieiFull Text
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