Himachal Journal of Agricultural Research

P. N. Sharma ¹, Anuradha Sharma ¹, M. Singh ¹

Affiliations
1 Department of Plant Pathology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, IN

Abstract

The study was conducted to assess the losses caused by leaf crinkle disease in terms of quantity and quality in urdbean in Himachal Pradesh. The disease is caused by urdbean leaf crinkle virus (ULCV) which consists of filamentous virus particles. There was significant reduction in yield components (cv. T-9) viz., plant height (23.8%), inter-nodal length (20.0%), pods/plant (70.0%), pod length (18.8%), seeds/pods (37.5%) and seed weight/plant (25.0%) as compared to healthy plants. Seed quality parameters viz., germination (45%), seed viability (86%), seed vigour (0.74%) and protein content (21%) were severely affected by virus infection. The severe reduction in various yield and quality parameters in the infected seed crop clearly envisages the need to control the virus by adopting effective control measures.

Keywords

Leaf Crinkle, Urdbean, Seed Viability, Seed Vigour.

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P. N. Sharma ¹, Kapil Patiyal ¹, Nidhi Rialch ¹, Deepika Rana ¹

Affiliations
1 Department of Plant Pathology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, IN

Abstract

Extensive surveys of capsicum crop grown under polyhouse conditions in Kangra, Mandi, Kullu, Hamirpur and Bilaspur districts of Himachal Pradesh (HP) were carried out during 2011 to 2014. The symptoms on the diseased plants included mosaic, mottling, chlorosis, upward or downward curling and deformation of leaves and fruits along with stunting of plants. The disease samples indexed through DAS ELISA showed the presence of eight viruses namely Cucumber mosaic virus (CMV); Pepper mild mottle virus (PMMoV); Tobacco mosaic virus (TMV); Tomato spotted wilt virus (TSWV); Tomato yellow leaf curl virus (TYLCV); Potato virus Y (PVY); Pepper mottle virus (PepMoV); Pepper veinal mottle virus (PVMV) in 2013 while only four viruses viz. CMV, TSWV, PVY and PMMoV were found in 2014. The presence of viruses which were found to be prevalent in DAS-ELISA was confirmed with RT-PCR using coat protein (CP) specific primers. The highest incidence of 60 per cent was recorded in Bilaspur district. The presence of three commonly occurring viruses viz. CMV, TSWV and PMMoV was confirmed with RT-PCR which yielded the amplification product of size 162 bp, 575 bp and 730 bp, respectively.

Keywords

Pepper Mild Mottle Virus, Capsicum, Prevalence, Detection.

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Shabnam Katoch ¹, Abhishek Katoch ¹, Shiwali Dhiman ¹, Pratibha Sharma ¹, S. K. Rana ¹, P. N. Sharma ¹

Affiliations
1 Department of Plant Pathology, CSK Himachal Pradesh Krishi Vishvavidylaya, Palampur - 176 062, IN

Abstract

The predicted resistance genes present in three common bean landraces KRC-5, KRC-8 and Jawala were validated for their reaction against Colletotrichum lindemuthianum races prevalent in Himachal Pradesh. The landrace KRC-5 showed resistance to 17 races (3, 7, 87, 179, 211, 238, 259, 437, 503, 513, 529, 537, 737, 775, 935, 957 and 1395) while landrace KRC-8, possessed resistance against 7 races viz., 17, 437, 513, 529, 737, 957 and 1395. In the inheritance test, F₂/RIL populations obtained from Jawala X KRC-5 segregated into 3:1/1:1 ratio, further confirming the presence of single dominant resistance gene in land race KRC-5.

Keywords

Colletotrichum lindemuthianum, Virulences, KRC (Kinnaur Rajmash Collection), Inheritance, Resistance.

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Shiwali Dhiman ¹, Anila Badiyal ¹, Shabnam Katoch ¹, Anju Pathania ¹, S. K. Sharma ¹, P. N. Sharma ¹

Affiliations
1 Department of Plant Pathology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, IN

Abstract

Bean anthracnose is one of the major diseases leading to a significant yield losses in susceptible cultivars. Hence, development of widely adaptable anthracnose-resistant cultivars using land races is of immense importance to maintain the production and productivity of the crop in disease prone areas. Keeping this in view, the present investigation was planned to introgress resistance gene from a well known Himalayan land race KRC-8 (Baspa) in the background of high yielding susceptible variety Jawala, both belonging to Andean gene pool. The hybridization, advancement with single seed decent approach and phenotypic screening of F_2:8 recombinant inbred lines (RIL) against anthracnose resulted in identification of fifteen potential lines showing resistance to anthracnose. Evaluation of agronomic traits of these lines revealed JB-3 to be significantly better than the parental genotype Jawala for number of pods per plant (18.67), number of seeds per pod (6.67), pod length (13.9 cm) and leaflet length (15.50 cm). Similarly, weight for 100 seeds, which directly contributes towards seed yield of beans, ranged from 48.6g in JB-5-2-2 to a maximum of 52.1g in JB-16-2 which is almost at par with that of Jawala (52.3g). Hence these RILs can be used as resistant donor germplasm for future breeding endeavours.

Keywords

Bean Anthracnose, Jawala, KRC-8, Baspa, Agronomic Traits.

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Surender Singh Chandel ¹, P. N. Sharma ², Kamal Dev Sharma ¹

Affiliations
1 Department of Agricultural Biotechnology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, IN
2 Department of Plant Pathology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, IN

Abstract

Sugars, besides source of energy, also provide tolerance and acclimation to plants under abiotic stresses. In plants, sugars are transported via specialized protein molecules called as sugar transporters. One of the most ubiquitous soluble sugars in plants, glucose-6-phosphate, is transported from cytosol into the chloroplast by glucose-6-phosphate/phosphate translocators (GPTs). Search of chickpea sequences revealed existence of two phylogentically diverse GPT genes in chickpea named as GPT1 and GPT2. The gene GPT1 (coding region: 1200 bases, 41.58% GC content) is present on chromosome 5 whereas GPT2 (coding region: 1164 bases, 38.74% GC content) on chromosome 1. Of these two, GPT1 was not active in chickpea leaves whereas GPT2, under heat stress, over-expressed vis-à-vis control in leaves of heat-tolerant ICC 15614 and down-regulated in heatsusceptible ICC 10685 suggesting that GPT2 is associated with heat tolerance in chickpea. The GPT2 can be a potential candidate gene for heat-tolerance in chickpea.

Keywords

Chickpea, Cicer arietinum, Glucose-6-phosphate Transporter, Gene Expression, High Temperature, Heat Stress.

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