Refine your search
Collections
Co-Authors
Year
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Thakur, Sanchit
- CRISPR-A Powerful Functional Genomic Tool in Crop Improvement
Abstract Views :182 |
PDF Views:2
Authors
Affiliations
1 Department of Crop Improvement, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur - 176 062, IN
1 Department of Crop Improvement, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur - 176 062, IN
Source
Himachal Journal of Agricultural Research, Vol 45, No 1&2 (2019), Pagination: 96-99Abstract
CRISPR and CRISPR Associated (Cas) Proteins (CRISPR-Cas) system is a vital defense system found in bacteria and archea. CRISPR activity requires a set of Cas genes, found adjacent to the CRISPR, that codes for protein essential for immune response. CRISPR system works in three stages to carry out a full immune response to invading foreign DNA i.e. acquisition, expression and interference stage. Recently Type II CRISPR-Cas9 system has also been adapted to perform genome engineering by inducing double-strand breaks (DSBs) in host DNA that can be repaired by either non-homologous end-joining (NHEJ) or homology-directed repair (HDR). Although the full potential of CRISPR-Cas9 has not been harnessed so far, this technology has already brought revolutionary changes in genomic research. CRISPR-Cas9 system has great potential for both research and therapeutics; however, improvements can still be made in its speci?city, efficiency and off target effects. With the advancement, the use of this system is increased in model plants as well as other crop plants like Arabidopsis, tobacco, maize, rice, wheat, sorghum, barley, brassica, soybean etc.Keywords
CRISPR, Cas, Spacer, Repeat, Crop Improvement.Full Text
References
- Curtin SJ, Voytas DF and Stupar RM. 2012. Genome engineering of crops with designer nucleases. The Plant Genome 5 (2): 42-50.
- Guilinger JP, Thompson DB and Liu DR. 2014. Fusion of catalytically inactive Cas9 to FokI nuclease improves the speci? city of genome modi? cation. Nature Biotechnology 32: 577-582.
- Ishino Y, Shinagawa H, Makino K, Amemura M and Nakata A. 1987. Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in E coli and identification of the gene product. Journal of Bacteriology 169: 5429-5433.
- Jansen R, Embden JDAV, Gaastra W and Schouls LM. 1995. Identification of genes that are associated with DNA repeats in prokaryotes. Molecular Microbiology 43:1565-1575.
- Kim D, Alptekin B and Budak K. 2018. CRISPR/ Cas 9 genome editing in wheat. Functional and integrative Genomics 18: 31-41.
- Khandagale K and Nadaf A. 2016. Genome editing for targeted improvement of plants. Plant Biotechnology Report 4: 51-68.
- Li J, Zhang H, Si,X, Tian Y, Chen K and Liu J. 2017. Generation of thermosensistive male sterile maize by targeted knockout of ZMTMS gene. Journal of 5 Genetics and Genomics 44: 465-468.
- Mojica FJM, Ferrer C, Juez G and Rodrýguez-Valera F. 2002. Long stretches of short tandem repeats are present in the largest replicons of the Archaea Haloferax mediterranei and Haloferax volcanii and could be involved in replicon partitioning. Molecular Microbiology 17: 85–93.
- Rani R, Yadav P, Barbadinkar KM, Baliyan N, Malhotra EV, Singh VK, Kumar A and Singh D. 2016. CRISPR/Cas-9: A promising way to exploit genetic variations in the plants. Biotechnological Letters 38: 1991-2006.
- Shen C, Que Z, Xia Y, Tang N, Li D & He R. 2017. Rapid generation of genetic diversity by multiplex CRISPR/ Cas 9 genome editing in rice. China life Sciences 60: 506-515.
- Sternberg SH, Redding S, Jinek M, Greene EC and Doudna JA. 2014. DNA interrogation by the CRISPR RNA guided endonuclease Cas9. Nature 507: 62–67.
- Voytas DF. 2013. Plant genome engineering with sequence specific nucleases. Annual Review Plant Biology 64: 327-350.
- Wiles MV, Qin W, Cheng AW and Wang H. 2015. CRISPR-Cas9-mediated genome editing and guide RNA design. Mammalian Genome 26: 501510.
- Xu T, Li Y, Nostrand JDV, He Z and Zhou J. 2014. Cas9 based tool for targeted genome editing and transcriptional control. Applied and Environmental Microbiology 80: 1544-1552.