Journal of Horticultural Sciences

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Molecular Diversity Analysis in F3 Intergeneric Population of Papaya (Carica papaya L.)


Affiliations
1 Central Potato Research Station, Muthorai, Ooty, The Nilgiris, TN, India
2 Horticultural College and Research Institute for women, Navalur Kuttapattu, Trichy, TN, India
3 Dept. of Fruit crops, Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore – 641003, India
4 Dept. of Oil Seeds, Centre for Plant Breeding and Genetics, TNAU, Coimbatore, TN, India
 

Attempts were made to estimate molecular diversity present in F3 populations of intergeneric crosses between Carica papaya L. (Var. Pusa Nanha and CP 50) and Vasconcellea cauliflora. Molecular studies revealed that PCR amplification using five ISSR primers in 40 F3 progenies yielded 53 reproducible amplified bands. Of the 53 bands, 44 were polymorphic (83.02%). Polymorphic Information Content (PIC) value ranged between 0.90 (ISSR 807 x 810) and 0.66 (ISSR 834 x 810). Similarity coefficients based on five ISSR markers ranged from 0.05 to 0.96. Maximum similarity was observed for genotypes 1, 4 and 6 of Pusa Nanha x Vasconcellea cauliflora (0.96). Minimum similarity was observed between genotypes 3 and 14 of CP 50 x Vasconcellea cauliflora (0.04). This higher genetic diversity of papaya progenies stands to contribute to development of new varieties and, using the data, further hybridization and selection can be planned.

Keywords

Carica papaya, Vasconcellea cauliflora, Intergeneric Hybridization, Molecular Diversity.
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  • Molecular Diversity Analysis in F3 Intergeneric Population of Papaya (Carica papaya L.)

Abstract Views: 238  |  PDF Views: 135

Authors

R. Sudha
Central Potato Research Station, Muthorai, Ooty, The Nilgiris, TN, India
T. N. Balamohan
Horticultural College and Research Institute for women, Navalur Kuttapattu, Trichy, TN, India
K. Soorianathasundaram
Dept. of Fruit crops, Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore – 641003, India
N. Manivannan
Dept. of Oil Seeds, Centre for Plant Breeding and Genetics, TNAU, Coimbatore, TN, India

Abstract


Attempts were made to estimate molecular diversity present in F3 populations of intergeneric crosses between Carica papaya L. (Var. Pusa Nanha and CP 50) and Vasconcellea cauliflora. Molecular studies revealed that PCR amplification using five ISSR primers in 40 F3 progenies yielded 53 reproducible amplified bands. Of the 53 bands, 44 were polymorphic (83.02%). Polymorphic Information Content (PIC) value ranged between 0.90 (ISSR 807 x 810) and 0.66 (ISSR 834 x 810). Similarity coefficients based on five ISSR markers ranged from 0.05 to 0.96. Maximum similarity was observed for genotypes 1, 4 and 6 of Pusa Nanha x Vasconcellea cauliflora (0.96). Minimum similarity was observed between genotypes 3 and 14 of CP 50 x Vasconcellea cauliflora (0.04). This higher genetic diversity of papaya progenies stands to contribute to development of new varieties and, using the data, further hybridization and selection can be planned.

Keywords


Carica papaya, Vasconcellea cauliflora, Intergeneric Hybridization, Molecular Diversity.