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Proliferation and Rooting Efficiency Studies in Sour Cherry (Prunus cerasus) Using in Vitro Techniques


Affiliations
1 Division of Pomology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar - 191 121, India
 

Murashige and Skoog's medium (MS) and Woody plant (WP) medium supplemented with IBA and BAP at different concentrations were tested for culture proliferation in sour cherry. MS medium supplemented with 1 mg L-1 BAP + 0.1 mg L-1 IBA, and, Woody plant medium fortified with 2 mg L-1 BAP + 0.1 mg L-1 IBA recorded maximum proliferation efficiency. MS medium supplemented with BAP @ 0.1 mg L-1 and devoid of auxin recorded optimum elongation of micro-shoots. MS medium fortified with 2 mg L-1 IBA gave highest ischolar_maining percentage and average number of ischolar_mains per explant. BAP and IBA in MS medium were ideal for proliferation indices like number of shoots per explant, average length of shoots (mm) and percentage of shoots with desired length for ischolar_maining, and, can be used in a multiplication programme.

Keywords

Cherry, MS Medium, Woody Plant Medium (WPM), Indole–3-Butyric Acid, Benzylaminopurine.
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  • Proliferation and Rooting Efficiency Studies in Sour Cherry (Prunus cerasus) Using in Vitro Techniques

Abstract Views: 211  |  PDF Views: 149

Authors

S. R. Singh
Division of Pomology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar - 191 121, India
A. S. Sundouri
Division of Pomology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar - 191 121, India
M. K. Sharma
Division of Pomology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar - 191 121, India
K. K. Srivastava
Division of Pomology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar - 191 121, India
H. A. Dar
Division of Pomology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar - 191 121, India

Abstract


Murashige and Skoog's medium (MS) and Woody plant (WP) medium supplemented with IBA and BAP at different concentrations were tested for culture proliferation in sour cherry. MS medium supplemented with 1 mg L-1 BAP + 0.1 mg L-1 IBA, and, Woody plant medium fortified with 2 mg L-1 BAP + 0.1 mg L-1 IBA recorded maximum proliferation efficiency. MS medium supplemented with BAP @ 0.1 mg L-1 and devoid of auxin recorded optimum elongation of micro-shoots. MS medium fortified with 2 mg L-1 IBA gave highest ischolar_maining percentage and average number of ischolar_mains per explant. BAP and IBA in MS medium were ideal for proliferation indices like number of shoots per explant, average length of shoots (mm) and percentage of shoots with desired length for ischolar_maining, and, can be used in a multiplication programme.

Keywords


Cherry, MS Medium, Woody Plant Medium (WPM), Indole–3-Butyric Acid, Benzylaminopurine.