Journal of Horticultural Sciences

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Effects of Growth Regulators and Explant-Type on Agrobacterium-Mediated Transformation in Brinjal (Solanum melongena L.) cv. Manjarigota


Affiliations
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bangalore - 560 089, India
2 Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga, India
 

Effects of growth regulators and type of explants on transformation and in vitro morphogenetic responses of brinjal cv. Manjarigota were studied. Both hypocotyl and cotyledonary explants showed marked influence on in vitro morphogenetic responses after Agrobacterium co-cultivation. Hypocotyl explants showed callus initiation and regeneration responses earlier than cotyledonary leaves. Hypocotyl explants were found to be better than cotyledonary leaf explants in regenerating shoots after Agrobacterium co-cultivation. There was delay and reduction in both callus and regeneration responses in Agrobacterium co-cultivated explants. Hypocotyl explants showed the highest regeneration response on MS medium containing 2 μM BAP and 0.05 μM NAA while cotyledonary leaves did not show regeneration response after Agrobacterium co-cultivation. However, they showed green buds on MS medium containing 10 μM BAP and 1 μM NAA, which could not differentiate into shoots. Overall, hypocotyl explants were found better in regenerating shoots after Agrobacterium co-cultivation.

Keywords

Growth Regulators, Explant, Brinjal, Transformation.
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  • Effects of Growth Regulators and Explant-Type on Agrobacterium-Mediated Transformation in Brinjal (Solanum melongena L.) cv. Manjarigota

Abstract Views: 229  |  PDF Views: 123

Authors

D. P. Prakash
Division of Biotechnology, Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bangalore - 560 089, India
B. S. Deepali
Division of Biotechnology, Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bangalore - 560 089, India
R. Asokan
Division of Biotechnology, Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bangalore - 560 089, India
Y. L. Ramachandra
Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga, India
Lalitha Anand
Division of Biotechnology, Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bangalore - 560 089, India
Vageeshbabu S. Hanur
Division of Biotechnology, Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bangalore - 560 089, India

Abstract


Effects of growth regulators and type of explants on transformation and in vitro morphogenetic responses of brinjal cv. Manjarigota were studied. Both hypocotyl and cotyledonary explants showed marked influence on in vitro morphogenetic responses after Agrobacterium co-cultivation. Hypocotyl explants showed callus initiation and regeneration responses earlier than cotyledonary leaves. Hypocotyl explants were found to be better than cotyledonary leaf explants in regenerating shoots after Agrobacterium co-cultivation. There was delay and reduction in both callus and regeneration responses in Agrobacterium co-cultivated explants. Hypocotyl explants showed the highest regeneration response on MS medium containing 2 μM BAP and 0.05 μM NAA while cotyledonary leaves did not show regeneration response after Agrobacterium co-cultivation. However, they showed green buds on MS medium containing 10 μM BAP and 1 μM NAA, which could not differentiate into shoots. Overall, hypocotyl explants were found better in regenerating shoots after Agrobacterium co-cultivation.

Keywords


Growth Regulators, Explant, Brinjal, Transformation.