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Spices Biotechnology


Affiliations
1 Kerala Agricultural University, Thrissur, Kerala, India
2 Indian Institute of Spices Research, Marikunnu P.O., Kozhikode 673 012, Kerala, India
 

In recent times, biotechnological tools have supplemented various conventional approaches in conservation, characterization, improvement and utilization for increasing production and productivity of spices. In many spices, viable micropropagation technologies are available for commercial production and generation of disease - free planting material. Somaclonal variation is important in crops where natural variability is low and a few useful somaclonal variants have been identified in ginger, turmeric and vanilla. Protoplast technology is also available for capsicum, black pepper, fennel, fenugreek, garlic, saffron and peppermint. In vitro cryopreservation, Synseed and Micro-rhizome technologies are available for safe propagation, conservation, movement, and exchange of spices germplasm. Studies are in progress for in vitro production of flavour and colouring compounds like capsaicin, vanillin, anethole, crocin, picrocrocin, saffranal, etc. using immobilized and transformed cell cultures. Use of molecular markers for crop profiling, fingerprinting, molecular taxonomy, identification of duplicate hybrids, estimation of genetic fidelity and tagging of genes for marker aided selection (MAS) is gaining importance. Isolation of important and useful genes and development of transgenics is in the preliminary stage.

Keywords

Spice Crops, Micropropagation, Somaclonal Variation, DNA Fingerprinting, Secondary Metabolites.
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  • Spices Biotechnology

Abstract Views: 287  |  PDF Views: 207

Authors

K. V. Peter
Kerala Agricultural University, Thrissur, Kerala, India
K. Nirmal Babu
Indian Institute of Spices Research, Marikunnu P.O., Kozhikode 673 012, Kerala, India
D. Minoo
Indian Institute of Spices Research, Marikunnu P.O., Kozhikode 673 012, Kerala, India

Abstract


In recent times, biotechnological tools have supplemented various conventional approaches in conservation, characterization, improvement and utilization for increasing production and productivity of spices. In many spices, viable micropropagation technologies are available for commercial production and generation of disease - free planting material. Somaclonal variation is important in crops where natural variability is low and a few useful somaclonal variants have been identified in ginger, turmeric and vanilla. Protoplast technology is also available for capsicum, black pepper, fennel, fenugreek, garlic, saffron and peppermint. In vitro cryopreservation, Synseed and Micro-rhizome technologies are available for safe propagation, conservation, movement, and exchange of spices germplasm. Studies are in progress for in vitro production of flavour and colouring compounds like capsaicin, vanillin, anethole, crocin, picrocrocin, saffranal, etc. using immobilized and transformed cell cultures. Use of molecular markers for crop profiling, fingerprinting, molecular taxonomy, identification of duplicate hybrids, estimation of genetic fidelity and tagging of genes for marker aided selection (MAS) is gaining importance. Isolation of important and useful genes and development of transgenics is in the preliminary stage.

Keywords


Spice Crops, Micropropagation, Somaclonal Variation, DNA Fingerprinting, Secondary Metabolites.