Journal of Horticultural Sciences

Open Access Open Access  Restricted Access Subscription Access

Validation of Molecular Markers Genetically Linked to S-Cytoplasm and Restoration-of-fertility (Rf) Loci in Hot Pepper (Capsicum annuum L.)


Affiliations
1 ICAR- Indian Institute of Horticultural Research, Bengaluru-560 089, India
 

Existence of CGMS system in hot pepper is due to the rearrangements in the mitochondrial genome and is largely used in economized and pure F1 hybrid seed production around the world. The orf456, a new ORF present at flanking region of the coxII gene at the 3’ end, was distinguished male sterile cytoplasm in hot peppers along with atp6-2gene. In the current study, eighteen pepper genotypes (nine each of A and corresponding B lines) of varied origin were used to validate with two male sterile cytoplasm (S-cytoplasm) specific sequence characterised amplified region (SCAR) markers viz., atp6-2(875 bp) and orf456(456 bp) and one restoration-of-fertility (Rf) locus specific marker, CRF(550 bp). The results clearly showed that the presence of CMS-S-cytoplasm and absence of restoration-of-fertility (Rf) gene in the pepper genotypes studied and is comparable with the phenotypic data. In view of the outcomes it has been reasoned that the accessible S and Rf markers available in the public domain are reproducible and can be promptly utilized for marker assisted selection (MAS) in hot pepper crop improvement program.

Keywords

CGMS, Hot Pepper, Marker Assisted Selection, Mitochondria, ORF.
User
Notifications
Font Size

  • Budar F and Pelletier G (2001) Male Sterility in Plants; Occurrence, Determinism, Significance and Use. Life Science 324: 543-550.

  • Doyle J J, Doyle JL (1990) Isolation ofplant DNA from fresh tissue. Focus 12:13–15.

  • Grelon M, Budar F, Bonhomme S, Pelletier G (1994) Ogura cytoplasmic male-sterility (CMS)associated orf138 is translated into a mitochondrial membrane polypeptide in male sterile Brassica cybrids. Molecular Genetics and Genomics 243:540–547

  • Gulyas G, Pakozdi K, Lee JS, Hirata Y (2006) Analysis of fertility restoration by using cytoplasmic male sterile red pepper (Capsicum annuum L.) lines. Breeding Science 56:331– 334.

  • Hanson MR (1991) Plant mitochondrial mutations and malesterility. Annual Review of Genetics 25:461–486

  • Hanson M and Bentolila S (2004) Interactions of mitochondrial andnuclear genes that affect male gametophyte development.Plant Cell 16:S154S169

  • Ji J J, Huang W, Yin C, Gong Z H (2013) Mitochondrial cytochrome c oxidase and F1FoATPase dysfunction in peppers (Capsicum annuum L.) with cytoplasmic male sterility and its association with orf507 and atp6-2genes. International Journal Molecular Science 14:1050–1068.

  • Kim DH, Kim BD (2005) Development of SCAR markers for early identification of cytoplasmic male sterility genotype in chili pepper (Capsicum annuum L.). Molecules & Cells 20:416–422.

  • Kim DS, Kim DH, Yoo JY, Kim BD (2006) Cleaved amplified polymorphic sequence and amplified fragment length polymorphism markers linked to the fertility restorer gene in chili pepper (Capsicum annuum L.). Molecules & Cells 21:135–140.

  • Kim DH, Kang JG, Kim BD (2007) Isolation and characterization of the cytoplasmic male sterility-associated orf456 gene of chili pepper (Capsicum annuum L.). Plant Molecular Biology 63:519–532.

  • Kumar R, Kumar S, Dwivedi N, Kumar S, Rai A, Singh M, et al., (2009) Validation of SCAR markers, diversity analysis of male sterile (S-) cytoplasm and isolation of an alloplasmic Scytoplasm in Capsicum. Scientia Horticulturae. 120:167–172

  • Miller I and Bruns E (2016) The effect of disease on the evolution of females and the genetic basis of sex in populations with cytoplasmic male sterility. Proceedings. Biological Sciences. 283 : 20153035

  • Peterson P A (1958) Cytoplasmically inherited male sterility in Capsicum americana. Naturalist 92:111–119.

  • Pruitt K D and M R Hanson (1989) Cytochrome oxidase subunit II sequences in Petunia mitochondria: Two intron-containing genes and an intron-less pseudogene associated with cytoplasmic male sterility. Current Genetics. 16:281-291.

  • Pruitt K D and M R Hanson. (1991) Splicing of the Petunia cytochrome oxidase subunit II intron.Current Genetics. 19:191 197

  • Reddy MK., Sadashiva, A T, Deshpande, A A, (2002) Cytoplasmic male sterility in chilli (Capsicum annuum L.). Indian Journal of Genetics. 62:363–364

  • Schnable P S and Wise R P (1998) The molecular basis of cytoplasmic male sterility and fertility restoration. Trends in Plant Science 3:175180.

  • Zhang B X, Huang S W, Yang G M, Guo J Z (2000) Two RAPD markers linked to a major fertility restorer gene in pepper. Euphytica 113:155161.


Abstract Views: 428

PDF Views: 178




  • Validation of Molecular Markers Genetically Linked to S-Cytoplasm and Restoration-of-fertility (Rf) Loci in Hot Pepper (Capsicum annuum L.)

Abstract Views: 428  |  PDF Views: 178

Authors

K. K. Jessy Mol
ICAR- Indian Institute of Horticultural Research, Bengaluru-560 089, India
D. C. Lakshmana Reddy
ICAR- Indian Institute of Horticultural Research, Bengaluru-560 089, India
Y. B. Manoj
ICAR- Indian Institute of Horticultural Research, Bengaluru-560 089, India
K. Madhavi Reddy
ICAR- Indian Institute of Horticultural Research, Bengaluru-560 089, India

Abstract


Existence of CGMS system in hot pepper is due to the rearrangements in the mitochondrial genome and is largely used in economized and pure F1 hybrid seed production around the world. The orf456, a new ORF present at flanking region of the coxII gene at the 3’ end, was distinguished male sterile cytoplasm in hot peppers along with atp6-2gene. In the current study, eighteen pepper genotypes (nine each of A and corresponding B lines) of varied origin were used to validate with two male sterile cytoplasm (S-cytoplasm) specific sequence characterised amplified region (SCAR) markers viz., atp6-2(875 bp) and orf456(456 bp) and one restoration-of-fertility (Rf) locus specific marker, CRF(550 bp). The results clearly showed that the presence of CMS-S-cytoplasm and absence of restoration-of-fertility (Rf) gene in the pepper genotypes studied and is comparable with the phenotypic data. In view of the outcomes it has been reasoned that the accessible S and Rf markers available in the public domain are reproducible and can be promptly utilized for marker assisted selection (MAS) in hot pepper crop improvement program.

Keywords


CGMS, Hot Pepper, Marker Assisted Selection, Mitochondria, ORF.

References