Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Polyphenols in Cocoa Beans: A Potential Antioxidant


Affiliations
1 Cocoa Research Centre, Kerala Agricultural University, Vellanikkara, Thrissur (Kerala), India
     

   Subscribe/Renew Journal


Cocoa (Theobroma cacao L.) is a potent source of polyphenol. There are reports that polyphenol and antioxidant activity is positively correlated. The present study investigated the total fat content, polyphenol content and antioxidant activity of cocoa beans obtained from twenty different cocoa hybrids. Folin – Ciocalteau (FC) reagent method was used to determine total polyphenol content in cocoa beans. Antioxidant activity was expressed as per cent radical scavenging activity and it was found out using DppH assay. Hybrid PIV 45.4 was found to be superior with respect to polyphenol content and antioxidant activity. Correlation studies revealed that total polyphenol content and antioxidant activity is positively correlated (r=0.613).

Keywords

Cocoa, Theobroma cacao L., Hybrids, Polyphenol Content, Radical Scavenging Activity, Correlation.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Afoakwa, E.O., Quao, J., Takrama, J., Budu, A.S. and Saalia, F.K. (2013).Chemical composition and physical quality characteristics of Ghanaian cocoa beans as affected by pulp pre-conditioning and fermentation. J. Food Sci. Technol., 50 (6) : 1097-1105.
  • Aikpokpodion, P.E. and Dongo, L.N. (2010). Effects of fermentation intensity on polyphenols and antioxidant capacity of cocoa beans. Int. J. Sustain. Crop Prod., 5(4): 6670.
  • Amma, P.S., Nair, R.V., Lalithabai, E.K., Mallika, V.K., Minimol, J.S. and Abraham, K. (2009). Cocoa in India. Kerala Agricultural University, India, pp.72.
  • Asna, A.C. (2013). Performance analysis of selected accessions of cocoa (Theobroma cacao L.). M.Sc. Thesis, Kerala Agricultural University. Thrissur.
  • Gutteridge, J.M. and Halliwell, B. (2000). Free radicals and antioxidants in the year 2000: a historical look to the future. Annals New York Acad. Sci., 899(1): 136-147.
  • Hart, M.E. (1982). Diet in disease: VI- Restoratives: Coffee, Cocoa, Chocolate, The Hospital,13 (321): 116.
  • Ibriae, A. and Eavar, S. (2014). Phenolic compounds and antioxidant activity of cocoa and chocolate products. Bull. Chemists & Technologists of Bosnia and Herzegovina, 42 : 37-40.
  • Jalil, A. and Ismail, A. (2008). Polyphenols in cocoa and cocoa products: is there a link between antioxidant properties and health?. Molecules, 13(9): 2190- 2219.
  • Kim, H. and Keeney, P.G. (1984). Epicatechin content in fermented and unfermented cocoa beans. J. Food Sci., 49: 1090-1092.
  • Lee, K.W., Kim, Y.J., Lee, H.J. and Lee, C.Y. (2003). Cocoa has more phenolic phytochemicals and a higher antioxidant capacity than teas and red wine. J. Agric Food Chem., 51(25): 7292-7295.
  • Malhotra, S.K. and Apshara, E.S. (2017). Genetic resources of cocoa (Theobroma cacao L.) and their utilisation – an appraisal. Indian J. Genet., 77(2): 199- 213.
  • Martínez, R., Torres, P., Meneses, M. A., Figueroa, J. G., Pérez-Álvarez, J.A. and Viuda-Martos, M. (2012).Chemical, technological and in vitro antioxidant properties of cocoa (Theobroma cacao L.) co-products. Food Res. Int., 49(1): 39-45.
  • Monteiro, W.R., Lopez, U.V. and Clement, D. (2009).Genetic improvement in cocoa. In: Jain, S.M. and Priyadarshan, D (eds.), Breeding plantataion tree crops: Tropical species. springer science, Business Media, pp. 589- 626.
  • Mossu, G. (1992). Cocoa. Macmillan Press Ltd. 103p.
  • Nehlig, A. (2013). The neuroprotective effects of cocoa flavanol and its influence on cognitive performance. Br. J. Clin. Pharmacol., 73(3): 716-727.
  • Othman, A., Ismail, A., Ghani, N.A. and Adenan, I. (2007). Antioxidant capacity and phenolic content of cocoa beans. Food Chem., 100 (4): 1523-1530.
  • Prayoga, R.D., Murwani, R. and Anwar, S. (2013). Polyphenol extracts from low quality cocoa beans: antioxidant, antibacterial and food colouring properties. Int. Food Res. J., 20(6): 3275-3281.
  • Rice-Evans, C., Miller, N. and Paganga, G. (1997).Antioxidant properties of phenolic compounds. Trends Plant Sci., 2(4): 152 - 159.
  • Rossini, K., Noreña, C.P. and Brandelli, A. (2011). Changes in the color of white chocolate during storage: potential roles of lipid oxidation and non- enzymatic browning reactions. J. Food Sci. & Techn., 48 (3) : 305-311.
  • Rubeena, M. (2015). Analysis of bean characters in cocoa (Theobroma cacao L.) hybrids bred for bold beans. M.Sc. Thesis, Kerala Agricultural University, Thrissur, Kerala, India.
  • Sadasivam, S. and Manickam, A. (1996).Biochemical methods (2nd Ed.). New Age International Publishers, New Delhi,256pp.
  • Saliva, J.M.R., Darmin, N., Fernandez, Y. and Mitjavila, S. (1991). Oxygen free radical scavenger capacity in aqueous models of different procyanidins from grape seeds. J. Agric Food Chem., 39: 1549-1552.
  • Schinella, G.R., Tournier, H.A., Prieto, J.M., De Buschiazzo, P.M. and Rios, J.L. (2002). Antioxidant activity of antiinflammatory plant extracts. Life Sci., 70(9): 1023-1033.
  • Steinberg, F.M., Holt, R.R., Schmitz, H.H. and Keen, C.L. (2002). Cocoa procyanidin chain length does not determine ability to protect LDL from oxidation when monomer units are controlled. J. Nutr. Biochem., 13(11): 645-652.
  • Steinberg, F.M., Bearden, M.M. and Keen, C.L. (2003). Cocoa and chocolate flavonoids: implications for cardio-vascular health. J. Am. Dietetic Assoc., 103(2): 215-223.
  • Sun, T. and Ho, C.T. (2005). Antioxidant activities of buck wheat extract. Food Chem., 90: 743-749.
  • Veeresh, S.A. (2017). Genetic stock development for Phytophthora pod rot resistance in cocoa (Theobroma cacao L.). M.Sc. Thesis, Kerala Agricultural University, Thrissur, Kerala, India.
  • Zumbé, A. (1998). Polyphenols in cocoa: are there health benefits. Nutr. Bulletin, 23(1): 94-102.

Abstract Views: 469

PDF Views: 0




  • Polyphenols in Cocoa Beans: A Potential Antioxidant

Abstract Views: 469  |  PDF Views: 0

Authors

K. S. Shilpa
Cocoa Research Centre, Kerala Agricultural University, Vellanikkara, Thrissur (Kerala), India
J. S. Minimol
Cocoa Research Centre, Kerala Agricultural University, Vellanikkara, Thrissur (Kerala), India
B. Suma
Cocoa Research Centre, Kerala Agricultural University, Vellanikkara, Thrissur (Kerala), India
Gayathri Mohan
Cocoa Research Centre, Kerala Agricultural University, Vellanikkara, Thrissur (Kerala), India

Abstract


Cocoa (Theobroma cacao L.) is a potent source of polyphenol. There are reports that polyphenol and antioxidant activity is positively correlated. The present study investigated the total fat content, polyphenol content and antioxidant activity of cocoa beans obtained from twenty different cocoa hybrids. Folin – Ciocalteau (FC) reagent method was used to determine total polyphenol content in cocoa beans. Antioxidant activity was expressed as per cent radical scavenging activity and it was found out using DppH assay. Hybrid PIV 45.4 was found to be superior with respect to polyphenol content and antioxidant activity. Correlation studies revealed that total polyphenol content and antioxidant activity is positively correlated (r=0.613).

Keywords


Cocoa, Theobroma cacao L., Hybrids, Polyphenol Content, Radical Scavenging Activity, Correlation.

References