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Lone, Imtiyaz Ahmad
- Effect of Different Growth Regulator Combinations on Growth Rate of Explants in Walnut In Vitro Studies Using MS Medium
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Authors
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1 Regional Research Station (SKUAST-K), Wadura (J&K), IN
1 Regional Research Station (SKUAST-K), Wadura (J&K), IN
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International Journal of Agricultural Sciences, Vol 14, No 1 (2018), Pagination: 27-33Abstract
The present investigation on effect of different growth regulator combinations on growth rate of walnut (J. regia L.) studies using MS medium was carried out in order to document the available genetic variability in walnut germplasm and to select elite walnut genotypes possessing superior attributes and quality traits. During the survey, data were recorded on one hundred fifty two (152) walnut trees growing in different areas of Kashmir valley. The study also involved establishment of response of elite walnut selections to different plant growth regulators in shoot morphogenesis. Woody species have been found to be far more difficult to clone in vitro than herbaceous plants. Poor response of the explants from mature woody species to in vitro manipulation is usually associated with the problem of browning and explant necrosis. The present studies were conducted on forced explants from three walnut selections (SKUAST 002, SKUAST 008, SKUAST 010). Murashiage and Skoog’s basal medium supplemented with 0.3 mg/l-1 benzylamino purine and 0.1 mg/l-1 indole-3-butyric acid. The growth regulator combinations revealed low to medium effect on the growth rate of explants. The maximum growth rate of 2.49 was found in BAP 0.3 mgl-1+IBA 0.1 mgl-1 followed by 2.19 in BAP 1.2 mgl-1+IBA 0.1 mgl-1 as compared to 1.46 and 1.27 in the BAP 0.9 mgl-1+IBA 0.1 mgl-1 and BAP 0.6 mgl-1+IBA 0.1 mgl-1, respectively. This difference was significant in both T1 (BAP 0.3 mgl-1+IBA 0.1 mgl-1) and T4 (BAP 1.2 mgl-1+IBA 0.1 mgl-1) as compared to both T2 (BAP 0.6 mgl-1+IBA 0.1 mgl-1) and T3 (BAP 0.9 mgl-1+IBA 0.1 mgl-1). Rest of the comparison among the growth regulator combinations were non-significant.Keywords
Walnut, Variability, Shoot Morphogenetic Response, Growth Rate of Walnut.
References
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- Estimation of Variability and Genetic Parameters for Nut Yield in the Seedling Raised Natural Walnut (Juglans regia L.) Population in the Kashmir Valley
Abstract Views :211 |
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Authors
Affiliations
1 Regional Research Station (SKUAST-K), Wadura (J&K), IN
1 Regional Research Station (SKUAST-K), Wadura (J&K), IN
Source
International Journal of Agricultural Sciences, Vol 14, No 1 (2018), Pagination: 97-101Abstract
The present investigation on estimation of variability and genetic parameters for nut yield (kg tree-1) in the seedling raised natural walnut (Juglans regia L.) population in the Kashmir valley’ was carried out in order to document the available genetic variability in walnut germplasm and to select elite walnut genotypes possessing superior attributes and quality traits. During the survey, data were recorded on one hundred fifty two (152) walnut trees growing in different areas of Kashmir valley. Remarkable variability was observed in seedling walnut trees for different morphological, nut and kernel characters. Similarly, variations were also reported for other characters viz., tree vigour, growth habit, branching habit, leaflet shape, shoot colour, nut shape, shell texture, shell colour, shell seal, shell strength, shell integrity, kernel shrivel and kernel colour. In order to reduce the variation in the nut yield the entire population of the seedling raised walnut trees in the present study were put into different age groups viz., 15-30, 31-50, 51-70 and above 70 years age on the basis of information from the owner and visual estimate (tree girth and canopy). Mean dried nut yield of 20.92±1.95; 31.35±2.35; 42.02±2.67 and 63.24±3.10 kg tree-1 was recorded for 15-30, 31-50, 51-70 and above 70 year age groups, respectively. The magnitude of variability recorded was 10.2- 55.5; 19.5-68.0, 30.3-165.5 and 37.5-185.0 kg tree-1, respectively.Keywords
Walnut, Variability, Nut Yield.References
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