International Journal of Plant Protection

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In vitro Evaluation of Fungicides, Bio-Control Agents and Plant Extracts against Early Blight of Tomato Caused by Alternaria solani (Ellis and Martin) Jones and Grout


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1 Department of Plant Protection, Aligarh Muslim University, Aligarh (U.P.), India
     

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The present experiment was conducted to test the efficacy of fungicides, bio-control agents and plant extracts in vitro against Alternaria solani causing early blight of tomato. Seven fungicides viz., four systemic (Propiconazole, Azoxystrobin, Thiophanate methyl and Carbendazim) and three non-systemic (Mancozeb, Captan and Zineb) at four concentrations i.e. 50, 100, 150 and 200 ppm and seven plant extracts viz., Datura strumarium (Jimson weed), Allium sativum (Garlic), Azadirachta indica (Neem), Zingiber officinale (Ginger), Ocimum sanctum (Tulsi), Calotropis gigantea (Aak) and Eucalyptus chamadulonsis (Eucalyptus) also at four concentrations i.e. 5, 10, 15 and 20 per cent were evaluated through poison food technique. Seven bio-control agents viz., Trichoderma harzianum, T. viride, T. koningii, T. hamatum, T. atroviride, Aspergillus niger and A. flavus were also evaluated in this study through dual culture technique. Among the systemic fungicides, Propiconazole was proved to be highly effective and recorded cent per cent inhibition at their all concentrations while among the non-systemic, Mancozeb was proved to be effective at their all concentrations but recorded 100 per cent inhibition only at their higher concentration i.e. 400 ppm. Among different plant extracts used, Azadirachta indica (Neem) was significantly inhibit the mycelial growth of pathogen at all concentrations followed by Datura strumarium (Jimson weed) and Calotropis gigantea (Aak). Of all bio-control agents, highest inhibition of radial growth of test fungus was recorded in Trichoderma harzianum (80.37%) followed by T. viride (71.48%) and T. koningii (77.41 %). However, T. hamatum (27.41%) was least effective in this study.

Keywords

Fungicides, Bio-Control Agents, Plant Extracts against, Early Blight of Tomato, Alternaria solani.
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  • In vitro Evaluation of Fungicides, Bio-Control Agents and Plant Extracts against Early Blight of Tomato Caused by Alternaria solani (Ellis and Martin) Jones and Grout

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Authors

Vaibhav Pratap Singh
Department of Plant Protection, Aligarh Muslim University, Aligarh (U.P.), India
R. U. Khan
Department of Plant Protection, Aligarh Muslim University, Aligarh (U.P.), India
Devesh Pathak
Department of Plant Protection, Aligarh Muslim University, Aligarh (U.P.), India

Abstract


The present experiment was conducted to test the efficacy of fungicides, bio-control agents and plant extracts in vitro against Alternaria solani causing early blight of tomato. Seven fungicides viz., four systemic (Propiconazole, Azoxystrobin, Thiophanate methyl and Carbendazim) and three non-systemic (Mancozeb, Captan and Zineb) at four concentrations i.e. 50, 100, 150 and 200 ppm and seven plant extracts viz., Datura strumarium (Jimson weed), Allium sativum (Garlic), Azadirachta indica (Neem), Zingiber officinale (Ginger), Ocimum sanctum (Tulsi), Calotropis gigantea (Aak) and Eucalyptus chamadulonsis (Eucalyptus) also at four concentrations i.e. 5, 10, 15 and 20 per cent were evaluated through poison food technique. Seven bio-control agents viz., Trichoderma harzianum, T. viride, T. koningii, T. hamatum, T. atroviride, Aspergillus niger and A. flavus were also evaluated in this study through dual culture technique. Among the systemic fungicides, Propiconazole was proved to be highly effective and recorded cent per cent inhibition at their all concentrations while among the non-systemic, Mancozeb was proved to be effective at their all concentrations but recorded 100 per cent inhibition only at their higher concentration i.e. 400 ppm. Among different plant extracts used, Azadirachta indica (Neem) was significantly inhibit the mycelial growth of pathogen at all concentrations followed by Datura strumarium (Jimson weed) and Calotropis gigantea (Aak). Of all bio-control agents, highest inhibition of radial growth of test fungus was recorded in Trichoderma harzianum (80.37%) followed by T. viride (71.48%) and T. koningii (77.41 %). However, T. hamatum (27.41%) was least effective in this study.

Keywords


Fungicides, Bio-Control Agents, Plant Extracts against, Early Blight of Tomato, Alternaria solani.

References