International Journal of Plant Protection

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In vitro Evaluation of Bio Agents Against Rice Sheath Blight Pathogen Rhizoctonia solani Kuhn


Affiliations
1 Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi (U.P.), India
2 Department of Plant Pathology, G. B. Pant University of Agriculture and Technology, Pantnagar (Uttarakhand), India
     

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It is now an established fact that strains of Trichoderma and Pseudomonas are most capable among all the potent biological control agents against phytopathogenic microorganisms recorded till now. They are effective against pathogenic microbes by the virtue of their capacity to produce some antagonistic chemicals (antibiosis), competition for food and nutrition, direct killing (parasitisation) etc. One such study was conducted to know the interaction between Trichoderma spp. against Rhizoctonia solani causing sheath blight disease in rice. Nine isolates of Trichoderma were tested for its parasitisation ability under dual culture shown and it was found that 77.03 – 100 per cent inhibition of Rhizoctonia after 72 hours of inoculation. Also, the effect of inhibiting volatiles was seen by inverted plate technique. It was found that none of the nine isolate of Trichoderma significantly reduced the growth of R. solani by producing volatiles and inhibition percent was found only 3.88 – 5.60 per cent. Three isolates of Pseudomonas florescens inhibited the growth of the test fungus by only 30.36-34.81per cent, which is very less when compared to fungal biocontrol agent Trichoderma.

Keywords

Biological Control, Pseudomonas Florescens, Rhizoctonia Solani, Sheath Blight of Rice, Trichoderma Harzianum.
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  • Akoto, O., Andoh, H., Darko, G., Eshun, K. and Osei-Fosu, P. (2013). Health risk assessment of pesticides residue in maize and cowpea from Ejura, Ghana.Chemosphere., 92 (1): 67-73.

  • Bonmann, J.M., Kush, G. S. and Nelson, R.J. (1992).Breeding for resistance to pests. Annu Rev Phytopathol., 30: 507–523.

  • Cu, R.M., Mew, T.W., Cassman, K.G. and Teng, P.S. (1996). Effect of sheath blight on yield in tropical, intensive rice production system. Plant Disease., 80 (10):1103-1108.

  • Dennis, C. and Webster, J. (1971). Antagonistic properties of species groups of Trichoderma. Transactions of British Mycological Society, 57: 25-39.

  • Gaigole, A.H., Wagh, G.N. and Khadse, A.C. (2011). Antifungal activity of Trichoderma species against soil borne pathogen. Asiatic J. Biotechnol. Resour., 2 (04) : 461-465.

  • Jardim, A.N. and Caldas, E.D. (2012). Brazilian monitoring programs for pesticide residues in food–Results from 2001 to 2010. Food Control, 25 (2) : 607-616.

  • Jia, Y., Correa-Victoria, F., McClung, A., Zhu, L., Liu, G., Wamishe, Y. and Correll, J. C. (2007). Rapid determination of rice cultivar responses to the sheath blight pathogen Rhizoctonia solani using a micro-chamber screening method. Plant Disease, 91(5) : 485-489.

  • Khan, M.R. and Khan, S.M. ( 2001). Biomanagement of Fusarium wilt of tomato by the soil application for certain phosphorus solubilizing micro-organism. Int. J. Pest Manag., 47: 227–231.

  • Marchetti, M.A. and Bollich, C.N. (1991). Quantification of the relationship between sheath blight severity and yield loss in rice. Plant Dis., 75:773-775.

  • Maruthasalam, S., Kalpana, K., Kumar, K.K., Loganathan, M., Poovannan, K., Raja, J. A. J. and Balasubramanian, P. (2007). Pyramiding transgenic resistance in elite indica rice cultivars against the sheath blight and bacterial blight. Plant Cell Reports., 26(6): 791-804.

  • Morton, D.J. and Stroube, W.H. (1955). Anatagonistic and stimulating effects of soil micro-organisms upon Sclerotium. Phytopathology, 45 : 417–420.

  • Nandakumar, R., Babu, S., Viswanathan, R., Raguchander, T. and Samiyappan, R . (2001). Induction of systemic resistance in rice against sheath blight disease by Pseudomonas fluorescens. Soil Biology & Biochemistry, 33: 603-612.

  • Qin, Z. and Zhang, M. (2005). Detection of rice sheath blight for in-season disease management using multispectral remote sensing. Appl. Earth Observation & Geoinformation, 7: 115– 128.

  • Rabindran, R. and Vidhyasekaran, P. (1996). Development of a formulation of Pseudomonas iluorescens PfALR2 for management of rice sheath blight. Crop Protection, 15: 715– 721.

  • Rao, M.S. (2007). Production of bioagents colonised vegetable seedlings for sustainable nematode managenent. International Conference on Indigenous Vegetable and Legume prospectus for Fighting Poverty, Hunger and Malnutrition. ISHA Acta Horticulturae, 752: 545–548.

  • Rathore, H.S. and Nollet, L.M. ( 2012). (ed): Pesticides: Evaluation of environmental pollution. Boca Raton: CRC Press. pp 147- 259.

  • Rush, M.C. and Lee, F.N. (1992). Sheath blight. In: Compendium of rice diseases. R. K. Webster and P. S. Gunnell, eds. The American Phytopathological Society. St. Paul, MN.pp. 22-23.

  • Savary, S.,Willocquet, L., Elazegui, F.A., Castilla, N.P. and Teng, P.S. (2000). Rice pest constraints in tropical asia: quantification of yield losses due to rice pests in a range of production situations. Plant Dis., 84:357–369.

  • Singh, A., Malhotra, S.K. and Singh, A. (1994). Effect of temperature and pH on growth and sclerotia formation of Rhizoctonia solani causing web blight of winged bean. Agricultural-Science-Digest-Karnal, 14(3-4): 141-142.

  • Vibha (2011). Evaluation of fungal and bacterial bioagents against Rhizoctonia solani on Solanum melongena (L.). Archives of Phytopathology & Plant Protection, 44 (8) : 743 - 750.


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  • In vitro Evaluation of Bio Agents Against Rice Sheath Blight Pathogen Rhizoctonia solani Kuhn

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Authors

Shrishti Lingwal
Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi (U.P.), India
Vishwanath
Department of Plant Pathology, G. B. Pant University of Agriculture and Technology, Pantnagar (Uttarakhand), India

Abstract


It is now an established fact that strains of Trichoderma and Pseudomonas are most capable among all the potent biological control agents against phytopathogenic microorganisms recorded till now. They are effective against pathogenic microbes by the virtue of their capacity to produce some antagonistic chemicals (antibiosis), competition for food and nutrition, direct killing (parasitisation) etc. One such study was conducted to know the interaction between Trichoderma spp. against Rhizoctonia solani causing sheath blight disease in rice. Nine isolates of Trichoderma were tested for its parasitisation ability under dual culture shown and it was found that 77.03 – 100 per cent inhibition of Rhizoctonia after 72 hours of inoculation. Also, the effect of inhibiting volatiles was seen by inverted plate technique. It was found that none of the nine isolate of Trichoderma significantly reduced the growth of R. solani by producing volatiles and inhibition percent was found only 3.88 – 5.60 per cent. Three isolates of Pseudomonas florescens inhibited the growth of the test fungus by only 30.36-34.81per cent, which is very less when compared to fungal biocontrol agent Trichoderma.

Keywords


Biological Control, Pseudomonas Florescens, Rhizoctonia Solani, Sheath Blight of Rice, Trichoderma Harzianum.

References