International Journal of Plant Protection

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In vitro Evaluation of Bio Agents Against Rice Sheath Blight Pathogen Rhizoctonia solani Kuhn


Affiliations
1 Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi (U.P.), India
2 Department of Plant Pathology, G. B. Pant University of Agriculture and Technology, Pantnagar (Uttarakhand), India
     

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It is now an established fact that strains of Trichoderma and Pseudomonas are most capable among all the potent biological control agents against phytopathogenic microorganisms recorded till now. They are effective against pathogenic microbes by the virtue of their capacity to produce some antagonistic chemicals (antibiosis), competition for food and nutrition, direct killing (parasitisation) etc. One such study was conducted to know the interaction between Trichoderma spp. against Rhizoctonia solani causing sheath blight disease in rice. Nine isolates of Trichoderma were tested for its parasitisation ability under dual culture shown and it was found that 77.03 – 100 per cent inhibition of Rhizoctonia after 72 hours of inoculation. Also, the effect of inhibiting volatiles was seen by inverted plate technique. It was found that none of the nine isolate of Trichoderma significantly reduced the growth of R. solani by producing volatiles and inhibition percent was found only 3.88 – 5.60 per cent. Three isolates of Pseudomonas florescens inhibited the growth of the test fungus by only 30.36-34.81per cent, which is very less when compared to fungal biocontrol agent Trichoderma.

Keywords

Biological Control, Pseudomonas Florescens, Rhizoctonia Solani, Sheath Blight of Rice, Trichoderma Harzianum.
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  • In vitro Evaluation of Bio Agents Against Rice Sheath Blight Pathogen Rhizoctonia solani Kuhn

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Authors

Shrishti Lingwal
Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi (U.P.), India
Vishwanath
Department of Plant Pathology, G. B. Pant University of Agriculture and Technology, Pantnagar (Uttarakhand), India

Abstract


It is now an established fact that strains of Trichoderma and Pseudomonas are most capable among all the potent biological control agents against phytopathogenic microorganisms recorded till now. They are effective against pathogenic microbes by the virtue of their capacity to produce some antagonistic chemicals (antibiosis), competition for food and nutrition, direct killing (parasitisation) etc. One such study was conducted to know the interaction between Trichoderma spp. against Rhizoctonia solani causing sheath blight disease in rice. Nine isolates of Trichoderma were tested for its parasitisation ability under dual culture shown and it was found that 77.03 – 100 per cent inhibition of Rhizoctonia after 72 hours of inoculation. Also, the effect of inhibiting volatiles was seen by inverted plate technique. It was found that none of the nine isolate of Trichoderma significantly reduced the growth of R. solani by producing volatiles and inhibition percent was found only 3.88 – 5.60 per cent. Three isolates of Pseudomonas florescens inhibited the growth of the test fungus by only 30.36-34.81per cent, which is very less when compared to fungal biocontrol agent Trichoderma.

Keywords


Biological Control, Pseudomonas Florescens, Rhizoctonia Solani, Sheath Blight of Rice, Trichoderma Harzianum.

References