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Pathogenic and Molecular Variability among Brassica Isolates of Alternaria brassicae Collected from Different Agro-Climatic Regions of India
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The Alternaria blight is one of the most destructive fungal disease of Indian mustard causes severe damage to the crop. Ten isolates of A. brassicae were collected from various agro-climatic location of India viz., Uttar Pradesh (Ab1), Madhya Pradesh (Ab2), Uttarakhand (Ab3), Bihar (Ab4), Jharkhand (Ab5), West Bengal (Ab6), Haryana (Ab7), Rajasthan (Ab8), Chhattisgarh (Ab9) and Gujarat (Ab10) and characterized for pathogenic and molecular variations. All the isolates showed high level of variability. The incubation period of the isolates was recorded on B. juncea 3 to 4 days, B. carinata 6.17 to 6.83 days, B. napus 5.17 to 6.00 days, B. nigra 4.17 to 5.17 days and in B. campestris it was ranged from 3.17 to 4.00 days. The results revealed that the maximum PDI was noted on Brassica juncea followed by B. campestris var yellow sarson, B. nigra, B. napus and B. carinata. Based on PDI ten isolates could be classified into three groups in which group one consist of isolates Ab3, Ab6, Ab7 and Ab5. Isolates Ab8, Ab2 and Ab4 fall in second group; while group three include isolates Ab1, Ab9 and Ab10. The dendrogram analysis identified two major clusters with 82 per cent similarity. One cluster (group I) comprised of 3 isolates (Ab1, Ab10 and Ab2). Whereas, another cluster (group II) comprised of Ab3, Ab6, Ab7, Ab5, Ab4, Ab8 and Ab9 at 86 per cent similarity. The three isolates (Ab3, Ab6 and Ab7) of group II showed 100 per cent similarity based on molecular basis.
Keywords
Pathogenic, Molecular Variability, Alternaria brassicae, Brassica Spp.
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