International Journal of Plant Protection

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Effect of UVC Rays on Biomass Production by Nomuraea rileyi (Farlow) Samson when Mixed with Various Adjuvants


Affiliations
1 Agricultural Research Station, Niphad, Nasik (M.S), India
     

   Subscribe/Renew Journal


The effect of UVC rays on the viability of entomopathogenic fungus, Metarhizium anisopliae (Metschinikoff) Sorokin, in the presence of various concentrations of adjuvants comprising glycerol (1.0, 2.0, 3.0 and 5.0%), tween-80 (0.5 and 1.0%), boric acid (1.0, 2.0 and 3.0%), carboxymethyl cellulose (0.5 and 1.0%), indigo (0.5 and 1.0%), turmeric (0.5 and 1.0%), molasses (0.5 and 1.0%), honey (1.0 and 2.0%), milk (1.0 and 2.0%), sunflower oil (0.5 and 1.0%), groundnut oil (0.5, 1.0 and 2.0%), mustard oil (0.5 and 1.0%), soybean oil (0.5 and 1.0%) and ghee (0.5 and 1.0%) and formulations without adjuvants, when exposed for 10 to 50 minutes, 2, 3 and 5 hours was studied under laboratory conditions. The UVC rays proved detrimental to the fungus and the effect increased with increase in exposure period. After 5 hours exposure to UVC rays, N.r.+SFO 1.0 per cent produced highest (4.97g) biomass when it was in rest of the treatments (2.40 to 4.87g) against 1.80g in control (N.r. alone). The next promising treatments were N.r.+SFO 0.5 per cent (4.87g), N.r.+GNO 1.0 per cent (4.87g) and N.r.+GNO 2.0 per cent (4.77g). The control N.r.alone without UVC exposure produced 7.30g of fungal biomass. Among the various oils sunflower and groundnut oil, among chemical adjuvant glycerol 2.0 per cent, CMC 0.50 per cent, boric acid and among nutrient sources honey, milk act as appreciable UVC protectant.

Keywords

Nomuraea rileyi, Media, Yeast Extract, Ultraviolet Formulations, Biomass.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Alves, R.T., Bateman, R.P., Prior, C. and Leather, S.R. (1998). Effect of simulated solar radiation on conidial germination of Metarhizium anisopliae in different formulations.Crop Protection, 17 (8) : 675-679.

  • Barad, A.H., Kapadia, M.N. and Jethva, D.M. (2014a).Residual toxicity of Nomuraea rileyi (Farlow) Samson against Helicoverpa armigera (Hubner) on pigeonpea. Internat. J. Plant Protec., 7(1) : 246-247.

  • Barad, A.H., Kapadia, M.N. and Jethva, D.M. (2014b). Influence of pesticides on the entomogenous fungus Nomuraea rileyi (Farlow) Samson. Internat. J. agric. Sci., 10 (2): 600-602.

  • Braga, G.U.L., Flint, S.D., Messias,C.L., Anderson,A.J. and Roberts,D.W. (2001). Effect of UV-B on conidia and germlings of the entomopathogenic hyphomycete Metarhizium anisopliae. Mycological Research, 105 (7) : 874-882.

  • Francisco,E.A., Rangel,D.E.N., Scala-Junior,N.la., Barbosa,J.C. and Correia, A.C.B. (2008). Exposure of Metarhizium anisopliae conidia to UV-B radiation reduces its virulence. J. Anhui Agri. University, 35 (2) : 246-249.

  • Hunt,T.R., Moore,D., Higgins, P.M. and Pruir, C. (1994). Effect if sunscreens, irradiance and resting periods on the germination of Metarhizium flavoviridae conidia. Entomophaga, 39 (314): 313-322.

  • Ignoffo, C.M., Hostetter, D.L., Sikorowski,P.P., Sutter,G. and Brooks,W.M. (1977). Inactivation of representative species of entomopathogenic viruses, a bacterium, fungus and protozoan by an ultraviolet light source. Environm. Entomol., 6: 411-415.

  • Kachhadiya, N.M., Kapadia, M.N. and Jethva, D.M. (2014). Field efficacy of Nomuraea rileyi (Farlow) Samson alone and in combination with insecticides against Spodoptera litura (Fabricius) infesting groundnut. Internat. J. Plant Protec., 7(1) : 143-146.

  • Kaur,G., Padmeja,V. and Sasikala,V. (1999). Control of Insect pest on cotton through mycopesticide formulations. Indian J. Microbiol., 39 : 169-173.

  • Leland,J.E. (2005). Characteristiscs of Beauveria bassiana isolates from Lyguslineolaris populations of Mississippi. J. Agric. Urban Entomol., 9: 667-702.

  • Lingappa, S. and Patil, R.K. (2002), Nomuraea rileyi – A PotentialMycoinsecticide.University of Agricultural Sciences, Dharwad, 30 p.

  • Lomer,C.J., Bateman,R.P., Johnson,D.L., Langewaid,J. and Thomas, M. (2001). Biological control of locusts and grasshoppers. Annual Rev. Entomol., 46: 667-702.

  • Moore, D., Bridge, P.D., Higgins, P.M., Bateman, R.P. and Prior, C. (1993). Ultra-violet radiation damage toMetarhizium flavoviridae conidia and the protection given by vegetable and mineral oils and chemical sunscreens. Ann. Appl. Biol., 122: 605-616.

  • Rangel,D.E.N. and Roberts, D.W. (2007). Inducing UV-B tolerance of Metarhizium anisopliae var. anisopliae conidia results in a trade-off between conidial production and conidial stress tolerance. J. Anhui Agric. Univ., 34 (2): 195-202.

  • Roberts, D.W. and Campbell, A.S. (1977). Stability of entomopathogenic fungi. In : "Environmental Stability of Microbial Insecticides, Sympopsium 1974" (C.M.Ignoffo and D.L.Hostetter, eds). Misc. Publ. Ent. Soc. Am., 10 : 19-76.

  • Sharmila, T. and Manjula, K. (2015). Field evaluation of oil formulations of Nomuraea rileyi (Farlow) Samson against Spodoptera litura and Helicoverpa armigera in groundnut. Internat. J. Plant Protec., 8(1) : 142-147.

  • Sharmila, T., Manjula, K. and Krishna, T. Murali (2015). Evaluation of oil formulations of Nomuraea rileyi (Farlow) Samson against Spodoptera litura under laboratory conditions. Internat. J. Plant Sci., 10 (1): 29-32.

  • Vimla Devi, P.S., Prasad, Y.G. and Chowdary, A. (2002). Effect of drying and formulation of conidia on virulence of entomofungal pathogen, Nomuraea rileyi (F.) Samson. J. Biological Control, 16 : 43-48.

  • Zimmermann,G. and Butin,H. (1973). Unter suchungen uber die Hitze- and Trockenresistent holzbewohnender Pilze. Flora, 162 : 393-419.


Abstract Views: 388

PDF Views: 0




  • Effect of UVC Rays on Biomass Production by Nomuraea rileyi (Farlow) Samson when Mixed with Various Adjuvants

Abstract Views: 388  |  PDF Views: 0

Authors

S. D. Patil
Agricultural Research Station, Niphad, Nasik (M.S), India
A. P. Padhye
Agricultural Research Station, Niphad, Nasik (M.S), India

Abstract


The effect of UVC rays on the viability of entomopathogenic fungus, Metarhizium anisopliae (Metschinikoff) Sorokin, in the presence of various concentrations of adjuvants comprising glycerol (1.0, 2.0, 3.0 and 5.0%), tween-80 (0.5 and 1.0%), boric acid (1.0, 2.0 and 3.0%), carboxymethyl cellulose (0.5 and 1.0%), indigo (0.5 and 1.0%), turmeric (0.5 and 1.0%), molasses (0.5 and 1.0%), honey (1.0 and 2.0%), milk (1.0 and 2.0%), sunflower oil (0.5 and 1.0%), groundnut oil (0.5, 1.0 and 2.0%), mustard oil (0.5 and 1.0%), soybean oil (0.5 and 1.0%) and ghee (0.5 and 1.0%) and formulations without adjuvants, when exposed for 10 to 50 minutes, 2, 3 and 5 hours was studied under laboratory conditions. The UVC rays proved detrimental to the fungus and the effect increased with increase in exposure period. After 5 hours exposure to UVC rays, N.r.+SFO 1.0 per cent produced highest (4.97g) biomass when it was in rest of the treatments (2.40 to 4.87g) against 1.80g in control (N.r. alone). The next promising treatments were N.r.+SFO 0.5 per cent (4.87g), N.r.+GNO 1.0 per cent (4.87g) and N.r.+GNO 2.0 per cent (4.77g). The control N.r.alone without UVC exposure produced 7.30g of fungal biomass. Among the various oils sunflower and groundnut oil, among chemical adjuvant glycerol 2.0 per cent, CMC 0.50 per cent, boric acid and among nutrient sources honey, milk act as appreciable UVC protectant.

Keywords


Nomuraea rileyi, Media, Yeast Extract, Ultraviolet Formulations, Biomass.

References