International Journal of Plant Sciences

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Effect of Hormonal Treatments on Haploid Formation and in Vitro Haploid Regeneration in Wheat × Maize System


Affiliations
1 Department of Genetics and Plant Breeding, College of Agriculture, G.B. Pant University of Agriculture and Technology, Pantnagar, U.S.Nagar (Uttarakhand), India
2 Central University of Crop Improvement, Umiam (Meghalaya), India
     

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Haploids are useful for basic studies on inter-genomic relationship, molecular studies and in practical breeding. In the present investigation post pollination treatment with different growth regulators and their combinations were analyzed. Twenty nine wheat F1’s and three maize parents were utilized to obtained haploid embryos. Three different hormonal treatments were designed to observe their effect on different parameters in wheat-maize haploid system. Out of three treatments, combination of GA3 and 2,4-D showed highly significant effect on caryopsis formation frequency (64.40), embryo formation frequency (18.84), embryo germination frequency (55.82) and haploid regeneration frequency (36.51). The growth pattern of haploid embryos on nutrient medium was quite different. The differential response of haploid embryos on same nutrient medium might be because of residual effect of different growth regulators.

Keywords

Wheat × Maize, Haploid Embryo, Hormonal Treatment, Residual Effect.
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  • Effect of Hormonal Treatments on Haploid Formation and in Vitro Haploid Regeneration in Wheat × Maize System

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Authors

Usha Pant
Department of Genetics and Plant Breeding, College of Agriculture, G.B. Pant University of Agriculture and Technology, Pantnagar, U.S.Nagar (Uttarakhand), India
V. K. Khanna
Central University of Crop Improvement, Umiam (Meghalaya), India

Abstract


Haploids are useful for basic studies on inter-genomic relationship, molecular studies and in practical breeding. In the present investigation post pollination treatment with different growth regulators and their combinations were analyzed. Twenty nine wheat F1’s and three maize parents were utilized to obtained haploid embryos. Three different hormonal treatments were designed to observe their effect on different parameters in wheat-maize haploid system. Out of three treatments, combination of GA3 and 2,4-D showed highly significant effect on caryopsis formation frequency (64.40), embryo formation frequency (18.84), embryo germination frequency (55.82) and haploid regeneration frequency (36.51). The growth pattern of haploid embryos on nutrient medium was quite different. The differential response of haploid embryos on same nutrient medium might be because of residual effect of different growth regulators.

Keywords


Wheat × Maize, Haploid Embryo, Hormonal Treatment, Residual Effect.

References