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Shankar, Uma
- Anti-Microbial Activity of Spices Black Cardamom, Mustard Seed and Liquorice against V. cholera, V. parahaemolyticus and V. vulnificus
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Authors
Affiliations
1 Department of Microbiology, Singhania University, Jhunjhunu (Rajasthan), IN
2 Division of Biotechnology, Cyto Gene Research and Development, Lucknow (U.P.), IN
1 Department of Microbiology, Singhania University, Jhunjhunu (Rajasthan), IN
2 Division of Biotechnology, Cyto Gene Research and Development, Lucknow (U.P.), IN
Source
International Journal of Plant Sciences, Vol 11, No 2 (2016), Pagination: 218-223Abstract
There are several water-borne diseases which are highly pathogenic for humans. Among all, Vibrio genus plays a major role. Vibrio cholerae, a gram negative anaerobe, is one of the most ancient and major water borne pathogen which causes fatal disease, cholera. This study chiefly focuses on the natural or plant derived products which could be used against Vibrio. In this work, 10 pond water samples were collected and investigated for presence or absence of pathogenic Vibrio species. A rapid method, multiplex PCR,of identification has been applied for determining the species. Out of 10, 8 samples showed presence of Vibrio species in them when cultured on selective media. The identified isolates of V. cholera, V. parahaemolyticus and V. vulnificus were then tested against three common spices, black cardamom(Amomum subulatum), mustard seed (Brassica nigra) and liquorice (Glycyrrhiza glabra) , extracts for their antibacterial property using agar well diffusion method. The antimicrobial assay result of spices showed a maximum activity by aqueous extract liquorice (Glycyrrhiza glabra) of 23 ± 0.52 (mm) against V.vulnificus followed by mustard seed (Brassica nigra) of 22.1 ± 0.60 (mm) against V.vulnificus.Keywords
Black Cardamom, Mustard Seed, Liquorice, mPCR, TOX Gene, Vibrio Species, Antibacterial Activity.
References
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- Isolation and Identification of Vibrio Species by Multiplex PCR and Investigation of its Prevention by Using Indian Spices
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Authors
Affiliations
1 Department of Microbiology, Sai Nath University, Ranchi (Jharkhand), IN
2 Division of Biotechnology, Cyto Gene Research and Development, Lucknow (U.P.), IN
3 Indian Council of Agricultural Research, Central Soil Salinity Research Institute Regional Research Station, Lucknow (U.P.), IN
1 Department of Microbiology, Sai Nath University, Ranchi (Jharkhand), IN
2 Division of Biotechnology, Cyto Gene Research and Development, Lucknow (U.P.), IN
3 Indian Council of Agricultural Research, Central Soil Salinity Research Institute Regional Research Station, Lucknow (U.P.), IN
Source
International Journal of Plant Sciences, Vol 11, No 2 (2016), Pagination: 307-314Abstract
The present study focuses the incidence of Vibrio spp. in water bodies which are used by people in surroundings for their daily needs. Vibrio constitute of both pathogenic and non-pathogenic species, thus, for differentiating between them a rapid method named multiplex PCR assay has been for the study. The method used was to first determine the physical and chemical constituents of all the 15 water samples (pond water) collected from different regions of Uttar Pradesh. Isolation of bacteria was done on selective media (TCBS) which showed both green and yellow colonies. These colonies were cultured separately on non selective media. All the pure cultures obtained were thus, used to extract their DNA using Phenol chloroform method. DNA was then visualized on agarose gel and quantified using double beam spectrophotometer. Samples with higher yield of DNA were used for amplification using species specific primers by multiplex PCR. The results were documented and thus, study derived the information that most of the waterbodies showed presence of pathogenic Vibrio species while few of them showed presence of non-pathogenic Vibrio spp.Keywords
mPCR, Vibrio, Chemical Parameters, Water, Amplification, Species Specific.References
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