Journal of Environment and Sociobiology

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Development of a Novel Consortium Using Bacteria With Multiple Plant Beneficial Traits From Over-Exploited Agricultural Soil


Affiliations
1 Department of Botany, Vivekananda College, Kolkata., India
2 Department of Microbiology, St. Xavier’s College (Autonomous), Kolkata., India
3 Department of Physics, St. Xavier’s College (Autonomous), Kolkata., India
     

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Soil is a treasure trove of myriad microbial communities that encompass a bewildering array of physiological, metabolic, and genomic diversity essential for sustenance of soil fertility. Over-exploitation of arable lands with extensive use of agrochemicals has negatively impacted soil structure and function by lowering crop productivity. Such damaged agro-ecosystem can be recovered through restoration of microbial richness to replenish various plant-beneficial services at community level. Incorporation of potential single or multiple plant-growth promoting bacteria (PGPB) in soil is a unique strategy in modern sustainable agriculture. In this study, PGPB strains were isolated from a long-term used agricultural soil of Bahadurpur, West Bengal. Only the bacterial isolates having nitrogen, phosphorus and potassium acquisition ability were further screened for multiple plant growth promoting (PGP) traits. Isolates S3, S5, and R1 showed nitrogen-fixing, phosphate and potassium solubilizing ability. They were tested for production of IAA, GA, ACC deaminase, siderophore, biofilm, lytic enzymes, and volatile biochemical compounds under in vitro condition. Three most potent isolates (S3, S5 and R1) were selected for development of a multi-strain consortium to be utilized as bio-inoculant. Based on 16s rRNA gene sequence they were identified as Bacillus zhangzhouensis strain MMAM, B. cereus strain MMAM3, and B. subtilis strain MMAM2.

Keywords

Long-Term Used Agricultural Soil, Plan-Growth Promoting Bacteria, Multi-Strain Consortium, Bio-Inoculant.
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  • Ahmad, F., Ahmad, I. and Khan, M. S. 2008. Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiol. Res., 163: 173-181. https://doi.org/10.1016/j.micres.2006.04.001.

  • Bakker, A. W. and Schippers, B. 1987. Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomonasspp.-mediated plant growth-stimulation. Soil Biol. Biochem., 19: 451-457. https:// doi.org/10.1016/0038-0717(87)90037-X.

  • Bhattacharyya, P. N. and Jha, D. K. 2012. Plant growth-promoting rhizobacteria (PGPR): emergence in agriculture. World J. Microbiol. Biotechnol., 28: 1327-1350. doi: 10.1007/s11274-011-0979-9.

  • Cappucino, J. G. and Sherman, N. 2005. Microbiology: A Laboratory Manual.7th Edition. Pearson, New York.

  • Dalvi, S. M. and Rakh, R. R. 2017. Siderophore producing Pseudomonas cf. monteilii for assured biological control of Sclerotium rolfsii causing stem rot of groundnut. Biosci. Discov., 8: 546-555.

  • Deb, P., Talukdar, S. A., Mohsina, K., Sarker, P. K. and Sayem, S. M. 2013. Production and partial characterization of extracellular amylase enzyme from Bacillus amyloliquefaciensP-001. Springer Plus., 2: 1-12. https://doi.org/10.1186/2193-1801-2-154.

  • Delgado-Baquerizo, M., Maestre, F. T., Reich, P. B., Jeffries, T. C., Gaitan, J. J., Encinar, D., Berdugo, M., Campbell, C. D. and Singh, B. K. 2016. Microbial diversity drives multifunctionality in terrestrial ecosystems. Nat. Commun., 7: 10541. DOI:10.1038/ncomms10541

  • Edi-Premono, M., Moawad, A. M. and Vleck, P. L. G. 1996. Effect of phosphate-solubilizing Pseudomonas putida on the growth of maize and its survival in the rhizosphere. Indonesian J. Crop Sci., 11: 13-23.

  • Freeman, D. J., Falkiner, F. R. and Keane, C. T. 1989. New method for detecting slime production by coagulase negative staphylococci. J. Clin. Pathol., 42: 872-874. http://dx.doi.org/10.1136/jcp.42.8.872.

  • Fukui, R., Schroth, M. N., Hendson, M. and Hancock, J. G. 1994. Interaction between strains of pseudomonads in sugar beet spermospheres and their relationship to pericarp colonization by Pythium ultimum in soil. Phytopathology., 84: 1322-1330.

  • Gouda, S., Kerry, R. G., Das, G., Paramithiotis, S., Shin, H.-S., and Patra, J. K. 2018. Revitalization of plant growth promoting rhizobacteria for sustainable development in agriculture. Microbiol. Res., 206: 131-140. DOI: 10.1016/j. micres.2017.08.016.

  • Gupta, P., Samant, K. and Sahu, A. 2012. Isolation of cellulose-degrading bacteria and determination of their cellulolytic potential. Int. J. Microbiol., 2012: 578925. https://doi.org/10.1155/2012/578925.

  • Hassani, M.A., Durán, P. and Hacquard, S. 2018 Microbial interactions within the plant holobiont. Microbiome.,6: 58. DOI:10.1186/s40168-018-0445-0

  • Holbrook, A. A., Edge, W. J. W. and Bailey, F. 1961. Spectrophotometric method for determination of gibberellic acid. Adv. Chem., 28: 159-167. https://doi. org/10.1021/ba-1961-0028.ch018.

  • Huang, R., Mcgrath, S., Hirsch, P., Clark, I., Storkey, J. and Wu, L. 2019. Plant– microbe networks in soil are weakened by century-long use of inorganic fertilizers.Microb. Biotechnol., 12: 1464-1475. DOI: 10.1111/1751-7915.13487.

  • Kimura, M., 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol., 16:111-120. https://doi.org/10.1007/BF01731581.

  • Kumar, P., Dubey, R. C. and Maheshwari, D. K. 2012. Bacillusstrains isolated from rhizosphere showed plant growth promoting and antagonistic activity against phytopathogens. Microbiol. Res., 167: 493-499. https://doi.org/10.1016/j. micres.2012.05.002.

  • Kumar, S., Stecher, G. and Tamura K. 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol. Biol. Evol., 33: 1870-1874. https:// doi.org/10.1093/molbev/msw054.

  • Maron, P. A., Sarr, A., Kaisermann, A., Lévêque , J., Mathieu, O., Guigue, J., Karimi, B., Bernard, L., Dequiedt, S., Terrat, S., Chabbi, A. and Ranjard, L. 2018. High microbial diversity promotes soil ecosystem functioning. Appl. Environ. Microbiol., 84(9): e02738-17. DOI: 10.1128/AEM.02738-17.

  • Maurya, B. R., Meena, V. S. and Meena, O. P. 2014. Influence of inceptisol and alfisol’s potassium solubilizing bacteria (KSB) isolates on release of K from waste mica. Vegetos., 27: 181-187. https://doi.org/10.5958/j.2229-4473.27.1.028.

  • Pande, A., Kaushik, S., Pandey, P. and Negi, A., 2020. Isolation, characterization, and identification of phosphate-solubilizing Burkholderia cepaciafrom the sweet corn cv. Golden Bantam rhizosphere soil and effect on growth-promoting activities. Int. J. Veg. Sci., 26: 591-607. https://doi.org/10.1080/19315260.201 9.1692121.

  • Pérez-Miranda, S., Cabirol, N., George-Téllez, R., Zamudio-Rivera,. L. S. and Fernández, F. J. O-CAS, a fast and universal method for siderophore detection. J. Microbiol. Methods., 70(1): 127-131. DOI: 10.1016/j.mimet.2007.03.023.

  • Pikovskaya, R. I. 1948. Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Microbiology., 17: 362-370.

  • Rahman, M. S., Islam, M. R., Mondol, O. K., Rahman, M. S., Sabrin, F. and Zohora, U. S., 2018. Screening of protease producing bacteria from tannery wastes of leather processing industries at Hazaribag, Bangladesh, Jahangirnagar. Univ. J. Biol. Sci., 7: 23-34. https://doi.org/10.3329/jujbs.v7i1.37970.

  • Rajawat, M. V. S., Singh, S., Tyagi, S. P. and Saxena, A. K. 2016. A modified plate assay for rapid screening of potassium-solubilizing bacteria. Pedosphere., 26: 768-773. https://doi.org/10.1016/S1002-0160(15)60080-7.

  • Rijavec, T. and Lapanje, A. 2016. Hydrogen cyanide in the rhizosphere: not suppressing plant pathogens, but rather regulating availability of phosphate. Front. Microbiol., 7: 1785. https://doi.org/10.3389/fmicb.2016.01785.

  • Sarker, A. and Al-Rashid, J. 2013. Analytical protocol for determination of Indole-3- acetic-acid (IAA) production by plant growth promoting bacteria (PGPB). Technical Report of Quantification of IAA by Microbes, pp. 1-2. https://www. researchgate.net/publication/263818523

  • Sharma, S., Sharma, A. and Kaur, M. and 2018. Extraction and evaluation of gibberellic acid from Pseudomonassp.: Plant growth promoting rhizobacteria. J. Pharmacogn. Phytochem., 7: 2790-2795.

  • Shilev, S., Azaizeh, H., Vassilev, N., Georgiev, D. and Babrikova, I. 2019. Interactions in soil-microbe-plant system: adaptation to stressed agriculture. In: D. Singh, V. Gupta and R. Prabha. (eds), Microbial Interventions in Agriculture and Environment. Springer, Singapore, pp 131-171. https://doi.org/10.1007/978-981-13-8391-5_6

  • Sinsabaugh, R. L., Lauber, C. L., Weintraub, M. N., Ahmed, B., Allison, S. D., Crenshaw, C., Contosta, A. R., Cusack, D., Frey, S., Gallo, M. E. and Gartner, T. B. 2008. Stoichiometry of soil enzyme activity at global scale. Ecol. Lett., 11: 1252-1264. https://doi.org/10.1111/j.1461-0248.2008.01245.x .

  • Sulistiyani, T. R. and Meliah, S. 2017. Isolation and characterization of nitrogen fixing endophytic bacteria associated with Sweet Sorghum (Sorghum bicolor). In: Proceedings The 1st SATREPS Conference, 1(1): 110-117.

  • Swamy, M. K., Akhtar, M. S. and Sinniah, U. R. 2016. Response of PGPR and AM fungi toward growth and secondary metabolite production in medicinal and aromatic plants. In: K. Hakeem and M. Akhtar (eds.), Plant, Soil and Microbes. Springer, Cham, pp. 145-168. https://doi.org/10.1007/978-3-319-29573-2_7.

  • TNAU Agritech Portal. 2013. Analytical techniques for soil testing: soil sampling, processing and storage. Soil sampling.https://agritech.tnau.ac.in/agriculture/ agri_soil_sampling.html.

  • Zhang, N., Yang, D., Wang, D., Miao, Y., Shao, J., Zhou, X., Xu, Z., Li, Q., Feng, H., Li, S. and Shen, Q. 2015. Whole transcriptomic analysis of the plant-beneficial rhizobacterium Bacillus amyloliquefaciensSQR9 during enhanced biofilm formation regulated by maize root exudates. BMC Genom., 16: 1-20. https://doi.org/10.1186/s12864-015-1825-5.


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  • Development of a Novel Consortium Using Bacteria With Multiple Plant Beneficial Traits From Over-Exploited Agricultural Soil

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Authors

Meenakshi Mukhopadhyay
Department of Botany, Vivekananda College, Kolkata., India
Arup Kumar Mitra
Department of Microbiology, St. Xavier’s College (Autonomous), Kolkata., India
Debapriya Maitra
Department of Microbiology, St. Xavier’s College (Autonomous), Kolkata., India
Bedaprana Roy
Department of Microbiology, St. Xavier’s College (Autonomous), Kolkata., India
Archisman Chakraborty
Department of Physics, St. Xavier’s College (Autonomous), Kolkata., India
Sudeshna Shyam Choudhury
Department of Microbiology, St. Xavier’s College (Autonomous), Kolkata., India
Indranath Chaudhuri
Department of Physics, St. Xavier’s College (Autonomous), Kolkata., India

Abstract


Soil is a treasure trove of myriad microbial communities that encompass a bewildering array of physiological, metabolic, and genomic diversity essential for sustenance of soil fertility. Over-exploitation of arable lands with extensive use of agrochemicals has negatively impacted soil structure and function by lowering crop productivity. Such damaged agro-ecosystem can be recovered through restoration of microbial richness to replenish various plant-beneficial services at community level. Incorporation of potential single or multiple plant-growth promoting bacteria (PGPB) in soil is a unique strategy in modern sustainable agriculture. In this study, PGPB strains were isolated from a long-term used agricultural soil of Bahadurpur, West Bengal. Only the bacterial isolates having nitrogen, phosphorus and potassium acquisition ability were further screened for multiple plant growth promoting (PGP) traits. Isolates S3, S5, and R1 showed nitrogen-fixing, phosphate and potassium solubilizing ability. They were tested for production of IAA, GA, ACC deaminase, siderophore, biofilm, lytic enzymes, and volatile biochemical compounds under in vitro condition. Three most potent isolates (S3, S5 and R1) were selected for development of a multi-strain consortium to be utilized as bio-inoculant. Based on 16s rRNA gene sequence they were identified as Bacillus zhangzhouensis strain MMAM, B. cereus strain MMAM3, and B. subtilis strain MMAM2.

Keywords


Long-Term Used Agricultural Soil, Plan-Growth Promoting Bacteria, Multi-Strain Consortium, Bio-Inoculant.

References