Journal of Environment and Sociobiology

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

DNA Barcoding Reveals Genetic Footprints of Multiple Putative Orthoptera Species (Insecta) in Assam, Northeast India


Affiliations
1 Bodoland University, Rangalikhata, Deborgaon, Assam-783370, India
2 Assam University, Silchar, Silchar, Assam-788011, India
3 InBOL Healthcare Educational Centre, Kolkata-700026, India
     

   Subscribe/Renew Journal


The orthopterans (grasshoppers, crickets, etc.) comprise one of the large orders of the class Insecta. As phytophagous some species of this order become important agricultural pests. Due to change in behaviour and appearance many of these insects seldom caused confusion in taxonomic identification of species. These confusions can largely be resolved by molecular characterization and DNA markers of the species. In this background a good number of orthopteran specimens from different localities and habitats of Assam state were selected for DNA barcode sequencing. A total of 1661 barcodes representing 369 species, including samples obtained from Northeast India, were evaluated for species delineation using several genealogical techniques like, Kimura 2-Parameter, Neighbor Joining, and Maximum Likelihood. Herein, we found 70% of the orthopteran species as straightforward instances, whereas 20 species exhibited cryptic diversity with intraspecies distances greater than the computed species delimitation threshold, i.e., 4.36%+ 0.9%. The remaining 90 species displayed interspecific genetic distance that is significantly lower than the estimated threshold, revealing ambiguous diversity. We also explored four putative species. This study has given new inputs to the taxonomic understanding of the Orthoptera by exploration of cryptic species. It points out also to the importance of DNA barcoding and molecular characterization of species, apart from the morphometric characteristics, in developing integrative taxonomy of insect species.

Keywords

Orthoptera, Northeast India, DNA Barcoding, Molecular Taxonomy, Phylogeny, Putative Species.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Altschul, S. F., Gish, W., Miller, W., Myers, E. W. and Lipman, D. J. 1990. Basic local alignment search tool. J. Mol. Biol., 215(3): 403-410.

  • Barrientos-Lozano, L., Rocha-Sánchez, A. Y., Fernández-Azuara, G. J., Sánchez-Reyes, U. J. and Almaguer-Sierra, P. 2020. Cryptic new species of Nesoecia Scudder, 1893 (Orthoptera: Tettigoniidae; Pseudophyllinae) from northeastern, Mexico. Zootaxa, 4859(4): 451-486.

  • Bhattacharjee, M. J., Khomdram, B. and Ghosh, S. 2011. DNA barcoding of ornamental fishes of North-East India. Assan University, Silcher India, 105 pp.

  • Bhattacharjee, M. J., Laskar, B. A., Dhar, B. and Ghosh, S. K. 2012. Identification and re-evaluation of freshwater catfishes through DNA barcoding. PloS one, 7: DOI: 10.1371. journal pone. 0049950.

  • Chen, L.-P., Chang, Y.-G., Li, J., Wang, J.-M., Liu, J.-L., et al. 2018. Application of DNA barcoding in the classification of grasshoppers (Orthoptera: Acridoidea) – a case study of grasshoppers from Hebei Province, China. Zootaxa, 4497(1): 99-110.

  • Cigliano, M., Braun, H., Eades, D. C. and Otte, D. 2020. Orthoptera species file. Version 5.0/5.0 [11. X. 2019].

  • Dhar, B. and Ghosh, S. K. 2015. Genetic assessment of ornamental fish species from North East India. Gene, 555: 382-392.

  • Dhar, B. and Ghosh, S. K. 2017. Mini-DNA barcode in identification of the ornamental fish: a case study from Northeast India. Gene, 627: 248-254.

  • Flook, P. and Rowell, C. H. 1997. The phylogeny of the Caelifera (Insecta, Orthoptera) as deduced from mtrRNA gene sequences. Mol. Phylogenet Evol., 8(1): 89-103.

  • Folmer, O., Black, M., Hoeh, W., Lutz, R. and Vrijenhoek, R. 1994. DNA primers for amplification of mitochondrial cytochrome C oxidase subunit I from diverae metazoan invertebrates. Mol. Mar. Biol. Biotechnol., 3: 294-299.

  • Guo, H.-F., Guan, B., Shi, F.-M. and Zhou, Z.-J. 2016. DNA Barcoding of genus Hexacentrus in China reveals cryptic diversity within Hexacentrus japonicus (Orthoptera, Tettigoniidae). ZooKeys, 596: 53-63.

  • Gwynne, D. T., 1995. Phylogeny of the Ensifera (Orthoptera): a hypothesis supporting multiple origins of acoustical signalling, complex spermatophores and maternal care in crickets, katydids, and weta. J. Orthoptera Res., 4: 203-218.

  • Hajibabaei, M., Janzen, D. H., Burns, J. M., Hallwachs, W. and Hebert, P. D. N. 2006. DNA barcodes distinguish species of tropical Lepidoptera. Proc. Natl. Acad. Sci. U. S. A., 103(4): 968-971.

  • Hebert, P. D. N., Cywinska, A., Ball, S. L. and DeWaard, J. R. 2003. Biological identifications through DNA barcodes. Proc. R. Soc. B: Biol. Sci., 270: 313-321.

  • Homchan, S. and Gupta, Y. M. 2022. The complete mitochondrial genome of giant cricket, Tarbinskiellus portentosus (Orthoptera: Gryllidae) and its curation. Mitochondrial DNA B Resour., 7(8): 1427-1431.

  • Huang, J., Zhang, A., Mao, S. and Huang, Y. 2013. DNA barcoding and species boundary delimitation of selected species of Chinese Acridoidea (Orthoptera: Caelifera). PloS One, 8(12): e82400. DOI: 10.1371.journal.pone.0082400.

  • Jago, N. D. 1998. The world-wide magnitude of Orthoptera as pests. J. Orthoptera Res., 7: 117-124.

  • Joern, A., Danner, B. J., Logan, J. D. and Wolesensky, W. 2006. Natural history of mass-action in predator-prey models: a case study from wolf spiders and grasshoppers. Am. Midl. Nat., 156(1): 52-64.

  • Kang, A. R., Kim, M. J., Park, I. A., Kim, K. Y. and Kim, I. 2016. Extent and divergence of heteroplasmy of the DNA barcoding region in Anapodisma miramae (Orthoptera: Acrididae). Mitochondrial DNA A: DNA Mapp. Seq., 27(5): 3405-3414.

  • Kaya, S., Ciplak, B., Chobanov, D. and Heller, K.-G. 2012. Poecilimon bosphoricus group (Orthoptera, Phaneropterinae): iteration of morpho-taxonomy by song characteristics. Zootaxa, 3225(1): 1-71.

  • Kociński, M., Grzywacz, B., Hristov, G. and Chobanov, D. 2021. A taxonomic outline of the Poecilimon affinis complex (Orthoptera) using the geometric morphometric approach. Peer J., 9: e12668. DOI: 10.771/peerj.12668.

  • Kumar, S., Stecher, G., Li, M., Knyaz, C. and Tamura, K. 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol. Biol. Evol., 35(6): 1547-1549.

  • Kundu, S., Kumar, H., Tyagi, K., Chandra, K. and Kumar, V. 2020. DNA barcoding of selected short-horned grasshoppers (Orthoptera: Acrididae) from Indian Himalayan region. Mitochondrial DNA B: Resour., 5(3): 3600-3605.

  • Le Gall, M., Overson, R. and Cease, A. 2019. A global review on locusts (Orthoptera: Acrididae) and their interactions with livestock grazing practices. Front. Ecol. Evol., 7: 263. DOI: 10.3389/fevo.2019.00263.

  • Lehmann, A., Devriese, H., Tumbrinck, J., Skejo, J., Lehmann, G. U. C. et al., 2017. The importance of validated alpha taxonomy for phylogenetic and DNA barcoding studies: a comment on species identification of pygmy grasshoppers (Orthoptera, Tetrigidae). ZooKeys, 679: 139-144.

  • Liu, Y.-J. and He, Z.-Q. 2021. A new species of the genus Parapentacentrus Shiraki, 1930 from Yunnan, China (Orthoptera: Gryllidae: Itarinae). Zootaxa, 5032(1): 143-146.

  • Mazumder, A., Dhar, B. and Ghosh, S. K. 2022. DNA-barcoding reveals cryptic diversity and re-evaluation of Ocypode (family: Ocypodidae) from the Sundarbans-UNESCO World Heritage Centre. Animal Gene, 25: 200127. https://doi.org/10.1016/j.angen.2022.200127.

  • Meier, R., Zhang, G. and Ali, F. 2008. The use of mean instead of smallest interspecific distances exaggerates the size of the “barcoding gap” and leads to misidentification. Syst. Biol., 57(5): 809-813.

  • Pickford, R. and Padgham, D. E. 1973. Spermatophore formation and sperm transfer in the desert locust, Schistocerca gregaria (Orthoptera: Acrididae). Can. Entomol., 105: 613-618.

  • Resh, V. H. and Cardé, R. T. 2009. Encyclopedia of Insects. Academic Press. Cambridge, MA.[Google Scholar].

  • Samways, M. J. and Sergeev, M. G. 1997. Orthoptera and landscape change. In: S. K. Gangwere, M. C. Muralirangan and M. Muralirangan (eds.), The Bionomics of Grasshoppers, Katydids, and their Kin. CABI, Wallingford, UK, pp. 147-162.

  • Tian, D., Shen, C.-Z., Chen, L., Chen, G.-Y., Zhang, T. et al. 2019. An integrative taxonomy of Vescelia pieli pieli species complex based on morphology, genes and songs from China (Orthoptera: Grylloidea: Phalangopsidae: Phaloriinae). Zootaxa, 4695: 67-75.

  • Tyagi, K., Kumar, V., Kundu, S., Pakrashi, A., Prasad, P. et al. 2019. Identification of Indian spiders through DNA barcoding: cryptic species and species complex. Sci. Rep., 9: 14033. https://doi.org/10.1038/s41598-019-50510-8.

  • Uluar, O., Yahyaoğlu, Ö. and Ciplak, B. 2021. Review of Uvarovistia (Orthoptera: Tettigoniidae; Tettigoniinae): genetic data indicate a new cryptic species, Uvarovistia munzurensis sp. n. Zootaxa, 4952(1): 152-168.

  • Wang, H., Jiang, B., Gu, J., Wei, T., Lin, L. et al. 2021. Molecular phylogeny and species delimitation of the genus Tonkinacris (Orthoptera, Acrididae, Melanoplinae) from China. Plos one 16(4): e0249431. DOI: 10.1371/journal.pone.0249431.

  • Ward, R. D., Zemlak, T. S., Innes, B. H., Last, P. R. and Hebert, P. D. N. 2005. DNA barcoding Australia’s fish species. Philos. Trans. R. Soc. Lond., B: Biol. Sci., 360: 1847-1857.

  • Zhao, L., Lin, L.-L. and Zheng, Z.-M. 2016. DNA barcoding reveals polymorphism in the pygmy grasshopper Tetrix bolivari (Orthoptera, Tetrigidae). ZooKeys, 582: 111-120.

  • Zhao, Y., Wang, H., Huang, H. and Zhou, Z. 2022. A DNA barcode library for katydids, cave crickets, and leaf-rolling crickets (Tettigoniidae, Rhaphidophoridae and Gryllacrididae) from Zhejiang Province, China. ZooKeys, 1123: 147-171.

  • Zhou, Z., Guo, H., Han, L., Chai, J., Che, X. et al. 2019. Singleton molecular species delimitation based on COI-5P barcode sequences revealed high cryptic/undescribed diversity for Chinese katydids (Orthoptera: Tettigoniidae). BMC Evol. Biol., 19: 79. DOI: https:// doi.org/10.1186/s12862-019-1404-5

  • Zhou, Z., Ye, H., Huang, Y. and Shi, F. 2010. The phylogeny of Orthoptera inferred from mtDNA and description of Elimaea cheni (Tettigoniidae: Phaneropterinae) mitogenome. J. Genet. Genomics, 37(5): 315-324.


Abstract Views: 280

PDF Views: 2




  • DNA Barcoding Reveals Genetic Footprints of Multiple Putative Orthoptera Species (Insecta) in Assam, Northeast India

Abstract Views: 280  |  PDF Views: 2

Authors

Meghali M. Narjari
Bodoland University, Rangalikhata, Deborgaon, Assam-783370, India
Ananya Mazumder
Assam University, Silchar, Silchar, Assam-788011, India
Raima Das
InBOL Healthcare Educational Centre, Kolkata-700026, India
Silistina Narzari
Bodoland University, Rangalikhata, Deborgaon, Assam-783370, India
Sankar K. Ghosh
Assam University, Silchar, Silchar, Assam-788011, India

Abstract


The orthopterans (grasshoppers, crickets, etc.) comprise one of the large orders of the class Insecta. As phytophagous some species of this order become important agricultural pests. Due to change in behaviour and appearance many of these insects seldom caused confusion in taxonomic identification of species. These confusions can largely be resolved by molecular characterization and DNA markers of the species. In this background a good number of orthopteran specimens from different localities and habitats of Assam state were selected for DNA barcode sequencing. A total of 1661 barcodes representing 369 species, including samples obtained from Northeast India, were evaluated for species delineation using several genealogical techniques like, Kimura 2-Parameter, Neighbor Joining, and Maximum Likelihood. Herein, we found 70% of the orthopteran species as straightforward instances, whereas 20 species exhibited cryptic diversity with intraspecies distances greater than the computed species delimitation threshold, i.e., 4.36%+ 0.9%. The remaining 90 species displayed interspecific genetic distance that is significantly lower than the estimated threshold, revealing ambiguous diversity. We also explored four putative species. This study has given new inputs to the taxonomic understanding of the Orthoptera by exploration of cryptic species. It points out also to the importance of DNA barcoding and molecular characterization of species, apart from the morphometric characteristics, in developing integrative taxonomy of insect species.

Keywords


Orthoptera, Northeast India, DNA Barcoding, Molecular Taxonomy, Phylogeny, Putative Species.

References