Journal of Environment and Sociobiology

Sankar Kumar Ghosh ¹, Prabal Ranjan Ghosh ², Tarikul Huda Mazumder ¹, Miranda Kshetrimayum ¹

Affiliations
1 Department of Biotechnology, Assam University, Silchar-788011, Assam, IN
2 Department of Vetarinary Physiology, WBUAFS, Kolkata-700 037, W.B., IN

Abstract

Northeast India is a reservoir of rich biodiversity, especially Greater one-horned rhinoceros (Rhinoceros unicornis) which is facing extinction. DNA barcoding being an exciting tool for accurate documention of biodiversity with a gene sequence, in the present article DNA barcode of R. unicornis is determined for the first time by amplifying mitochondrial COI gene region using cross species primer designing and other bioinformatics analysis (Accession No. JN41700). Phylogeny analysis of obtained sequence with other related species distinctly maintains the assigned taxonomic position.

Keywords

DNA Barcoding, Mitochondrial DNA, COI-5', Rhinoceros Unicornis

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Miranda Kshetrimayum ¹, Sankar Kumar Ghosh ², Monika Ahanthem ¹

Affiliations
1 DNA barcode and Genomics Laboratory, Department of Biotechnology, Assam University, Silchar-788011, Assam, IN
2 Department of Biotechnology, Assam University, Silchar-788011, Assam, IN

Abstract

Identification of forensic articles like carcasses or marketed products is difficult. Although recent molecular advancement provides use of fragments of cytochrome c oxidase I (COI) as species level marker in all animals, yet primers are diverse among groups of animals. So, primer selection for any unstructured specimen is difficult. We explored possibility of cross species primer to amplify the DNA barcode fragment of COI for a native horse species (Equus caballus). Different barcode primers of common taxa are empirically tested in silico. Based on the critical criteria of DNA barcoding resultantly forward primer for Bird DNA barcoding and reverse primer for Fish DNA gave an in silico PCR product of 699 bp, also verified in vitro using genomic DNA extracted from hair samples. The sequence similarity and homology search in BOLD nucleotide database confirm specific identification. This study forecasts application of cross species primer to identify suspected biological sample in diverse arena.

Keywords

Forensic Science, Cytochrome C Oxidase 1, Cross Species Primer, In Silico, Bold, Unstructured Specimen.

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