Journal of Environment and Sociobiology

Prabal Ranjan Ghosh ¹, Monika Ahanthem ², Pradosh Mahadani ², Amalesh Choudhury ³, Sankar Kumar Ghosh ²

Affiliations
1 West Bengal University of Animal and Fishery Sciences, Kolkata-700037, West Bengal, IN
2 Department of Biotechnology, Assam University, Silchar-788011, IN
3 S. D. Marine Institute, Sunderban, West Bengal, IN

Abstract

Chicken domestication plays an important role for major supply of animal protein throughout the world. In India, wide diversities of domestic chicken are descended from wild red jungle fowl (RJF) (Gallus gallus), the mother of all fowls of the world. In the present study, DNA barcode sequence and cytochrome c oxidase subunit I (COI) region of wild RJF from northeastern India using PCR are determined, sequenced and subsequently deposited in GenBank (Accession No. JN793568). The study provides for the first time the DNA barcodes of red jungle fowl from northeastern India and subsequent bioinformatics yield that the RJF is grouped within correct taxonomic assignment of other Gallus species proving strong resolving power of DNA barcode in chicken.

Keywords

Cytochrome C Oxidase Subunit I, DNA Barcodes, Red Jungle Fowl, Gallus gallus, Northeastern India.

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Miranda Kshetrimayum ¹, Sankar Kumar Ghosh ², Monika Ahanthem ¹

Affiliations
1 DNA barcode and Genomics Laboratory, Department of Biotechnology, Assam University, Silchar-788011, Assam, IN
2 Department of Biotechnology, Assam University, Silchar-788011, Assam, IN

Abstract

Identification of forensic articles like carcasses or marketed products is difficult. Although recent molecular advancement provides use of fragments of cytochrome c oxidase I (COI) as species level marker in all animals, yet primers are diverse among groups of animals. So, primer selection for any unstructured specimen is difficult. We explored possibility of cross species primer to amplify the DNA barcode fragment of COI for a native horse species (Equus caballus). Different barcode primers of common taxa are empirically tested in silico. Based on the critical criteria of DNA barcoding resultantly forward primer for Bird DNA barcoding and reverse primer for Fish DNA gave an in silico PCR product of 699 bp, also verified in vitro using genomic DNA extracted from hair samples. The sequence similarity and homology search in BOLD nucleotide database confirm specific identification. This study forecasts application of cross species primer to identify suspected biological sample in diverse arena.

Keywords

Forensic Science, Cytochrome C Oxidase 1, Cross Species Primer, In Silico, Bold, Unstructured Specimen.

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