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Mallick, Pijush
- Isolation of Mesophyll Protoplast from Indian Mulberry (Morus alba L) CV. S1635
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Authors
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1 Division of Plant Biology, Bose Institute, P-1/12, C.I.T., Scheme VII M, Kolkata-700 054, West Bengal, IN
1 Division of Plant Biology, Bose Institute, P-1/12, C.I.T., Scheme VII M, Kolkata-700 054, West Bengal, IN
Source
Journal of Environment and Sociobiology, Vol 13, No 2 (2016), Pagination: 217-222Abstract
Mulberry (Morus alba L. cv S1635) is a perennial crop plant in Indian sericulture and used for silkworm Bombyx mori L. feeding. Qualitative and quantitative improvement of this crop is highly essential in respect to better and quality silk production and hence through breeding approaches the development of new lines is important. Protoplasts are the good source of any plant to study about their genetics and cellular mechanisms for breeding. Using cell wall degradating enzymes mulberry protoplasts from mesophyll tissue were isolated. Several combinations of cellulose, macerozyme and driselase enzymes mixture were tested in different incubation conditions. A combination of Cellulase R10-1%, Macerozyme R10-1% and Driselase-0.25% in 0.6M mannitol showed comparatively better yield (3.8 × 106 / gm tissue) after 3-4 hrs shaking with 100 rpm at 28°C.Keywords
Mulberry Plant, Mesophyll Protoplast, Cell Wall Degradating Enzymes.
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- Analysis of Relative DNA Content of Termitomyces Protoplast using Propidium Iodide
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Authors
Affiliations
1 Department of Biotechnology, School of Biotechnology & Biosciences, Brainware University, 398, Ramkrishnapur Road, Barasat, North 24 Parganas, Kolkata-700125, IN
2 Division of Plant Biology, Bose Institute, P-1/12, CIT Rd, Scheme VIIM, Kankurgachi, Kolkata-700054, IN
1 Department of Biotechnology, School of Biotechnology & Biosciences, Brainware University, 398, Ramkrishnapur Road, Barasat, North 24 Parganas, Kolkata-700125, IN
2 Division of Plant Biology, Bose Institute, P-1/12, CIT Rd, Scheme VIIM, Kankurgachi, Kolkata-700054, IN
Source
Journal of Environment and Sociobiology, Vol 17, No 2 (2020), Pagination: 15-18Abstract
Towards establishment of biodiversity character via analysis of relative DNA content of wild edible mushroom, Termitomyces species is used here for the first time. Dye propidium iodide (PI), an intercalating fluorescent molecule with molecular mass of 668.4 Da is used for staining of Termitomyces protoplasts to analyse the cellular DNA content using flow cytometer. PI binds the cellular nucleic acids at 535 nm excitation and 617 nm emission range. The protoplasts were isolated from liquid MYG grown vegetative tissues of edible mushroom strain Termitomyces heimii. The isolated protoplasts were purified with 0.6M manitol (MW 3350) followed by washing in PBS buffer. Protoplast pellet was suspended in PI (l mg/ml) hypotonic solution and final volume made up with sterile water. Incubation was done at 37°C for 30 min followed by analyzing using flow cytometer. Results showed that staining depends upon the protoplast purity, size and viability. The vegetative tissue showed a heterogeneous protoplast population in terms of sizes which is ultimately reflected on the relative amount of DNA content. The heterogeneous protoplast population in the suspension through cytometry score results the unequal amount of DNA content in every cell meaning unequal size, age and viability. It is established that PI can be used for staining of mushroom protoplast in biodiversity and genetic research.Keywords
Edible mushroom, Protoplast, Propidium iodide, Flow cytometry, DNA content.- Analysis of Genetic Diversity of Protoplast Fused Mushrooms Pleurotus Florida X Lentinus Squorrosulus (PFLS) Somatic Hybrids through Rapd Markers
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Authors
Affiliations
1 Department of Biotechnology, School of Biotechnology & Biosciences, Brainware University, 398, Ramkrishnapur Road, Barasat, North 24 Parganas, Kolkata-700125, IN
2 Division of Plant Biology, Bose Institute, P-1/12, CIT Rd, Scheme VIIM, Kankurgachi, Kolkata-700054, IN
1 Department of Biotechnology, School of Biotechnology & Biosciences, Brainware University, 398, Ramkrishnapur Road, Barasat, North 24 Parganas, Kolkata-700125, IN
2 Division of Plant Biology, Bose Institute, P-1/12, CIT Rd, Scheme VIIM, Kankurgachi, Kolkata-700054, IN
Source
Journal of Environment and Sociobiology, Vol 17, No 2 (2020), Pagination: 37-43Abstract
A total of twelve somatic hybrid strains, Pleurotus florida x Lentinus squorrosulus (pfls), were characterized through PCR based RAPD analysis. Sixteen random decemer primers yielded a total of 136 fragments with 97.79 % polymorphism with an amplicon range of 200 bp - 3000 bp in size. RAPD-08 showed optimum amplification and generated 12 scorable bands (250 bp -1800 bp) where RAPD-07 amplified least and generated 5 scorable bands (400 bp - 1300 bp). PIC ranged from 0.424 (SRS-15) to 0.499 (SRS-16). Based on Jaccard’s proximity matrix the highest genetic distance was found in between parent L. squarrosulus and hybrid pfls1q which means they are genetically most distantly related. Among the hybrid pfls1k and pfls1p showed maximum genetic distance while the most closely related hybrids were pfls1i and pfls1l. The parent L. squarrosulus was most genetically distant individual than the other parent P. florida.Keywords
Hybrid Mushroom, Genetic Diversity, Polymorphism, RAPD.- Women Participation in Sericulture Activities of South 24 Parganas District, West Bengal: an Interim Report of an Ongoing Study
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Authors
Affiliations
1 Dinabandhu Andrews College, 54, Raja SC Mullick Road, Baishnabghata, Kolkata-700084, IN
2 Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, West Bengal-741252, IN
3 Department of Biotechnology, Brainware University, Barasat, North 24 Pgs, Kolkata-700125, IN
4 Raiganj University, University Road, College Para, Raiganj, West Bengal 733134, IN
1 Dinabandhu Andrews College, 54, Raja SC Mullick Road, Baishnabghata, Kolkata-700084, IN
2 Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, West Bengal-741252, IN
3 Department of Biotechnology, Brainware University, Barasat, North 24 Pgs, Kolkata-700125, IN
4 Raiganj University, University Road, College Para, Raiganj, West Bengal 733134, IN