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Ghosh, Sayantan
- Isolation of Mesophyll Protoplast from Indian Mulberry (Morus alba L) CV. S1635
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1 Division of Plant Biology, Bose Institute, P-1/12, C.I.T., Scheme VII M, Kolkata-700 054, West Bengal, IN
1 Division of Plant Biology, Bose Institute, P-1/12, C.I.T., Scheme VII M, Kolkata-700 054, West Bengal, IN
Source
Journal of Environment and Sociobiology, Vol 13, No 2 (2016), Pagination: 217-222Abstract
Mulberry (Morus alba L. cv S1635) is a perennial crop plant in Indian sericulture and used for silkworm Bombyx mori L. feeding. Qualitative and quantitative improvement of this crop is highly essential in respect to better and quality silk production and hence through breeding approaches the development of new lines is important. Protoplasts are the good source of any plant to study about their genetics and cellular mechanisms for breeding. Using cell wall degradating enzymes mulberry protoplasts from mesophyll tissue were isolated. Several combinations of cellulose, macerozyme and driselase enzymes mixture were tested in different incubation conditions. A combination of Cellulase R10-1%, Macerozyme R10-1% and Driselase-0.25% in 0.6M mannitol showed comparatively better yield (3.8 × 106 / gm tissue) after 3-4 hrs shaking with 100 rpm at 28°C.Keywords
Mulberry Plant, Mesophyll Protoplast, Cell Wall Degradating Enzymes.
References
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- Effect of Biochemicals from different Cultivars of Indian Mustard (Brassica Juncea L.) on the Population Abundance of Lipaphis Erysimi (Kalt.) in Laboratory Condition
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Authors
Affiliations
1 School of Biotechnology and Biosciences, Department of biotechnology, 398, Ramkrishnapur Road, Brainware University, Barasat, West Bengal: 700 125, IN
2 Bose Institute, Division of Plant Biology, P-1/12, C. I. T. Road, Scheme VII M, Kolkata-700 054, West Bengal, IN
1 School of Biotechnology and Biosciences, Department of biotechnology, 398, Ramkrishnapur Road, Brainware University, Barasat, West Bengal: 700 125, IN
2 Bose Institute, Division of Plant Biology, P-1/12, C. I. T. Road, Scheme VII M, Kolkata-700 054, West Bengal, IN
Source
Journal of Environment and Sociobiology, Vol 17, No 2 (2020), Pagination: 31-36Abstract
Population abundance of mustard aphid Lipaphis erysimi (Kalt.) was studied upon the treatment of aqueous extract from eight cultivars of Indian mustard Brassica juncea L. from different agronomical regions of India. A wild crucifer Rorippa indica, known for its aphid tolerant nature, also was studied, side by side. Treatment of R. indica extract on the isolated population of mustard aphids showed a significant suppressing effect on aphid population. Cultivar Gujarat Mustard 3 was appeared to be best host for the aphid, and cv. Ashirwad was seen to be most tolerant to L. erysimi as on the treatment of water extract from Gujarat Mustard 3.Nymphal growth of aphid was not affected and a regular growth pattern was observed here. But aqueous extract treatment from cv. Ashirwad significantly controlled aphid population on non-host Nicotiana tabacam.Keywords
Mustard Aphids, Brassica Juncea, Lipaphis Erysimi, Rorippa Indica, Aqueous Extract.- Standardization Of Bioethanol Production From The Well-known Weed, Water Hyacinth
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Authors
Affiliations
1 Department of Biotechnology, Brainware University, Barasat, Kolkata–700125,, IN
1 Department of Biotechnology, Brainware University, Barasat, Kolkata–700125,, IN
Source
Journal of Environment and Sociobiology, Vol 19, No 1 (2022), Pagination: 55-62Abstract
Water hyacinth (Eichhornia crassipes) is a very common weed that degrades inland water bodies and responsible for drastic water pollution. This weed possesses about 80% of cellulose within it which is a polysaccharide of the monomeric β-D glucose. Glucose is a good substrate for the production of bioethanol. This process can be accelerated using some microbial strains which are well documented. The microbes that can grow on the water extract of water hyacinth are believed to utilize cellulose as the organic carbon source. These microbes were isolated from the water hyacinth extract and plated on CMC agar and PDA media to get the pure culture. The bacterial colonies were found on CMC media resembled the Actinomycetaceae family and were used as the inoculating culture of the bioethanol production. The small scale pilot fermentation process, without the help of any bioreactor, was carried out using the water hyacinth as a raw material for one month. The presence of ethanol was confirmed using Iodoform test. The process was replicated to confirm the reliability of the results. This bioethanol production process may prove to be cheaper and eco-friendly.Keywords
Water hyacinth, Pollutant, BOD, Actinomycetaceae, Fermentation, Bioethanol, Green energy, Pollution control, Iodoform test.References
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