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Lalitha, K. G.
- High-Performance Thin Layer Chromatographic Method for the Simultaneous Quantitation of Melatonin and Zolpidem in Tablet formulation
Abstract Views :164 |
PDF Views:67
Authors
Affiliations
1 Department of Pharmaceutical Chemistry, JKKMMRF College of Pharmacy, B. Komarapalayam - 638 183, Tamil Nadu, IN
2 Department of Pharmaceutical Chemistry, ULTRA College of Pharmacy, Madurai - 625 020, Tamil Nadu, IN
1 Department of Pharmaceutical Chemistry, JKKMMRF College of Pharmacy, B. Komarapalayam - 638 183, Tamil Nadu, IN
2 Department of Pharmaceutical Chemistry, ULTRA College of Pharmacy, Madurai - 625 020, Tamil Nadu, IN
Source
Journal of Pharmaceutical Research, Vol 13, No 2 (2014), Pagination: 61-65Abstract
A simple, accurate and precise densitometry method for the simultaneous estimation melatonin (MEL) and zolpidem (ZOL) in pharmaceutical dosage forms has been developed and validated. Separation of drugs was carried out using toluene: n-butanol:glacial acetic acid:water (1:4:2:2v/v/v/v) as mobile phase on precoated Silica Gel 60F254 plates. The densitometry evaluation of spot was carried out at 254 nm. The Rf value for MEL and ZOL were found to be 0.87±0.01 and 0.59±0.01 respectively. The method was validated with respect to linearity, accuracy, precision and robustness as per the International Conference on Harmonization (ICH) guidelines. The drug response with respect to peak area was linear over the concentration range 150-900 ng/spot and 250-1500 ng/spot for MEL and ZOL respectively, limit of detection and limit of quantitation were found to be 14.96 ng/spot and 45.60 ng/spot for MEL and 19.53 ng/spot and 59.24 ng/spot for ZOL. The percentage recovery of MEL and ZOL were found be 99.04-99.35% and 98.11-98.77% respectively. The % RSD values for (intra-day RSD 0.26-0.56% and inter-day RSD 0.36-0.78 %for MEL and intraday RSD 0.18-0.31% and inter day 0.23-0.31% for ZOL). It was observed that the proposed HPTLC method could be used for efcient analysis and monitoring of the MEL and ZOL in combined tablet dosage forms.Keywords
Melatonin, Zolpidem, HPTLC, Validation.- Validated HPTLC Method for Simultaneous Estimation of Lafutidine and Domperidone in a Tablet Dosage Form
Abstract Views :152 |
PDF Views:78
Authors
Affiliations
1 Department of Pharmaceutical Analysis, JKKMMRF College of Pharmacy, B. Komarapalayam - 638 183, Tamil Nadu, IN
2 Department of Pharmaceutical Chemistry, Ultra College of Pharmacy, Madurai - 625 020. TamilNadu, IN
1 Department of Pharmaceutical Analysis, JKKMMRF College of Pharmacy, B. Komarapalayam - 638 183, Tamil Nadu, IN
2 Department of Pharmaceutical Chemistry, Ultra College of Pharmacy, Madurai - 625 020. TamilNadu, IN
Source
Journal of Pharmaceutical Research, Vol 13, No 2 (2014), Pagination: 66-70Abstract
A reliable simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for estimation of lafutidine (LF) and domperidone (DP) in combined tablet dosage forms. The analysis was performed on 10×20 cm aluminium-precoated plates coated with 0.2 mm layers of silica gel 60 F254 (E-Merck, Germany) with ethyl acetate:methanol:toluene:acetone:glacial acetic acid (1.0:1.5:4.0:2.0:0.2 v/v/v/v/v) as mobile phase. Camag TLC Scanner III was used for the UV densitometric scanning at 285 nm. This system was found to give a compact spot of LF and DP at retention factor (Rf) value of 0.32±0.03 and 0.65±0.02 respectively. Linear regression analysis data for the calibration curve showed good relationship with respect to peak area in the concentration 100-500ng per spot for both LF and DP with (r2 = 0.9993) and (r2 = 0.9985) for LF and DP respectively. Limit of detection (15.51 ng/spot for LF and 23.04 ng/spot for DP), limit of quantication (47.03 ng/spot for LF and 69.83 ng/spot for DP) were found. The proposed HPTLC method has potential applications in determination of lafutidine and domperidone in tablet formulations.Keywords
Lafutidine, Domperidone, HPTLC, Tablet Formulation.- Antihyperglycemic, Antihyperlipidemic and Antioxidant Activities of Ethanolic Extract of Helleborus niger Linn Roots in Streptozotocin - Nicotinamide Induced Diabetic Rats
Abstract Views :179 |
PDF Views:80
Authors
Affiliations
1 Department of Phytopharmacy and Phytomedicine, J. K. K. Munirajah Medical Research Foundation's Annai J. K. K. Sampoorani Ammal College of Pharmacy, B. Komarapalayam – 638 183, Namakkal District, Tamil Nadu, IN
2 Department of Pharmaceutical Chemistry, Ultra College of Pharmacy, Madurai – 625 020, Tamil Nadu, IN
1 Department of Phytopharmacy and Phytomedicine, J. K. K. Munirajah Medical Research Foundation's Annai J. K. K. Sampoorani Ammal College of Pharmacy, B. Komarapalayam – 638 183, Namakkal District, Tamil Nadu, IN
2 Department of Pharmaceutical Chemistry, Ultra College of Pharmacy, Madurai – 625 020, Tamil Nadu, IN