Journal of Scientific and Technical Research (Sharda University, Noida)

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

In Vitro Culture-An Alternative Method for the Enhanced Production of Some Volatile Components of Ocimum Citriodorum Vis


Affiliations
1 School of Sciences, Indira Gandhi National Open University, MaidanGarhi, New Delhi, India
2 Department of Biology, BCAS, University of Delhi, New Delhi, India
     

   Subscribe/Renew Journal


Ocimum plants are highly valued for their effective antibacterial, antifungal, antioxidant and therapeutic activities. These properties are attributed to some active metabolites. In order to enhance and develop an alternative source for the production of such active components, a comparative study was carried out. Composition of volatile organic compounds emitted by in vitro shoots, calli derived from leaf and nodal explant of Ocimum citriodorum Vis. were studied using Gas Chromatography-Mass Spectroscopy (GC-MS) and compared to those emitted by mother plants. Solvent extraction method was used for obtaining essential oils from the samples. Total 18 volatile compounds from the leaf extract of mother plant, 14 volatile compounds from the leaf extract of in vitro regenerated plants, 5 volatile compounds from the calli derived from leaf and 10 volatile compounds from the calli derived from nodal explant were identified.The content of nerol (2.44%) and geranylacetate (1.53%) was greater in in vitro regenerated plantlets than in comparison to mother plant (nerol- 0.88% and geranylacetate- 0.32%). Seven new compounds (isocaryophyllene, β-farnesene, β-himachalene, globulol, geranylformate, β-citronellylacetate, citronellylisobutyrate) were identified from the in vitro regenerated plants while six and three new compounds were identified from the calli derived from nodal explant (geraniol, trans-β-bergamotene, β-longipinene, bergamotene, geranylformate and β-citronellyacetate) and leaf (β-curcumene, trans-β-bergamotene, β-citronellol) respectively.From the present study, it can be concluded that in vitro propagated plants as well as calli derived from leaf and nodal segment may be used as an alternate source for the increased production of bioactive compounds.

Keywords

Ocimum Citriodorum Vis., GC-MS, Monoterpenes, Sesquiterpenes, Phenolic Terpenes, Terpene Esters.
User
Subscription Login to verify subscription
Notifications
Font Size

  • Arikat, N. A., Jawad, F. M., Karam, N. S., and Shibli, R. A. (2004). Micropropagation and accumulation of essential oils in wild sage (Salvia fruticosaMill.), Sci. Hortic., 100, 193-202.

  • Becker, H. (1970). Untersuchungen zur Frage der Bildung fluechtiger Soff wechset produckte in callus kultunen, Biochem. Physiol. Pflanz, 161, 425- 441.

  • Bodhipadma, K., Noichinda, S., Phromthara, T., Rutatip, S., and Nathalang, K. (2012). Determination of antioxidants from lemon basil callus during induction. The Journal of Applied Science, 11(2), 1-8.

  • Brown, J. T., and Charlwood, B. V. (1986). The accumulation of essential oils by tissue cultures of Pelargonium fragrans (Willd.). Federation of European Biochemical Societies, 204(1), 117- 120.

  • Burbott, A. J., and Loomis, W. D. (1967). Effects of light and temperature on the monoterpenes of peppermint. Plant Physiol., 42, 20-28.

  • Chan, L. K., Dewi, P. R., and Boey P. L. (2005). Effect of plant growth regulators on regeneration of plantlets from bud cultures of Cymbopogon nardus L. and the detection of essential oils from the in vitro plantlets. Journal of Plant Biology, 48(1), 142- 146.

  • Charles, D. J., and Simon, J. E. (1990). Comparison of extraction methods for the rapid determination of essential oil content and composition of basil. Journal of the American Society for Horticultural Science, 115(3), 458-462,

  • Janarthanam, B., and Sumathi, E. (2012). Plantlet regeneration from nodal explants of Ocimum citriodorum Vis.. Bangladesh Journal of Scientific and Industrial Research, 47(4), 433-436.

  • Kalkan, E. (2012). Chemical profiling and extraction processing of basil (Ocimum) cultivars grown in Oklahoma, Ph.D. Thesis, Oklahoma State University.

  • Liber, Z., Stanko, K. C., Politeo, O., Strikic, F., Kolak, I., Milos, M., and Satovic, Z. (2011). Chemical characterization and genetic relationships among Ocimum basilicum L. cultivars. Chemistry and Biodiversity, 8, 978-1989.

  • Lichtenthaler, H. K., Schwender, J., Disch, A., and Rohmer, M. (1997). Biosynthesis of isoprenoids in higher plant chloroplasts proceeds via a mevalonate-independent pathway. FEBS Lett., 400, 271-274.

  • Ma, X., and Gang, D. R. (2006). Metabolic profiling of turmeric (Curcuma longa L.) plants Derived from in vitro micropropagation and conventional green-house cultivation. Journal of Agricultural and Food Chemistry, 54 (25), 9573-9583.

  • McCaskill, D., and Croteau, R. (1999). Isopentenyl diphosphate is the terminal product of the deoxyxylulose-5-phosphate pathway for terpenoid biosynthesis in plants. Tetrahedron Letter, 40, 653-656,

  • Morone-Fortunato, I., and Avato, P. (2008). Plant development and synthesis of essential oils in micropropagated and mycorrhiza inoculated plants of Origanum vulgare L. ssp. hirtum (Link) Ietswaart. Plant Cell Tissue and Organ Culture, 93, 139-149.

  • Murashige, T., and Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant, 15, 473-479.

  • Nitnaware, K. M., Naik, D. G., and Nikam, T. D. (2011). Thidiazuron-induced shoot organogenesis and production of hepatoprotective lignin phyllanthin and hypophyllanthin in Phyllanthus amarus, Plant Cell Tissue and Organ Culture, 104, 101-110.

  • Paul, A., Thapa, G., Basu, A., Mazumdar, P., Kalita, M. C., and Sahoo, L. (2010). Rapid plant regeneration, analysis of genetic fidelity and essential aromatic oil content of micropropagated plants of Patchouli, Pogostemon cablin (Blanco) Benth.- an industrially important aromatic plant. Industrial Crops and Products, 32, 366-374.

  • Prideevech, P., Chumpolsri, W., Suttiarporn, P., and Wongporanchai. (201). The chemical composition and antioxidant activities of basil from Thailand using retention indices and comprehensive two dimensional gas chromatography. Journal of Serbian Chemical Society, 75(11), 1503-1513.

  • Predieri, S., and Rapparini, F. (2007). Terpene emission in tissue culture. Plant Cell, Tissue and Organ Culture, 91(2), 87-95.

  • Sangvanphet, T., Chareonviriyaphap, T., and Naksathit, A. (Repellent function of essential oils from Ocimum citriodorum on Aedes aegypti (Diptera: Culicidae) mosquitoes, a vector of dengue, Science Exhibition 2011, BI- P- 28, Science University.

  • Shin, S. H., Kim, Y. S., and Kang, C. A. (2001). Production of volatile oil components by cell culture of Agastache rugosa O. Kuntze. Natural Product Science, 7, 120-123,

  • Simon, J. E., Morales, M. R., Phippen, W. B., Vieira, R. F., and Hao, Z. (1999). Basil: a source of aroma compounds and a popular culinary and ornamental herb, Reprinted from: Perspectives on new crops and new uses, J. Janick (ed.), ASHS Press, Alexandria, VA,

  • Sood, H., and Chauhan, R. S. (2010). Biosynthesis and accumulation of a medicinal compound, Picroside-I, in cultures of Picrorhiza kurroa Royle ex Benth. Plant Cell Tissue and Organ Culture, 100, 113-117.

  • Sudria, C., Pinol, M. T., Palazon, J., Cusido, R. M., Vila, R., Morales, C., Bonfill, M., and Canigueral, S. (1999). Influence of plant growth regulators on the growth and essential oil content of cultured Lavandula dentataplantlets. Plant Cell Tissue and Organ Culture, 58, 177-184.

  • Tripathi, A., Abbas, N. S., and Nigam, A. (2014). Micropropagation of an endangered medicinal herb O. citriodorum Vis. Journal of Plant Development Sciences, 6(3), 365-374.

  • Tripathi, A. (2011). Anticarcinogenic potential of some medicinal plants. The Botanica, 59(61), 169-175.

  • Tusro, M., Inoue, M., and Kameoka, H. (2001). Variation in essential oil components in regenerated lavender (Lavendula vera DC) plants. Scientia Horticulturae, 88, 309-317.

  • Webb, J. K., Banthorpe, D. V., and Watson, D. G. (1984). Monoterpene synthesis in shoots regenerated from callus cultures. Phytochemistry, 23, 903-904.

  • Zielinska, S., Piatczak, E., Kalemba, D., and Matkowaski, A. (2011). Influence of plant growth regulators on volatiles produced by in vitro grown shoots of Agastache rugosa (Fischer and C.A. Meyer) O. Kuntze, Plant Cell, Tissue and Organ Culture, 107, 161- 167.

  • Zielinska, A., and Nowak, I. (2014). Fatty acids in vegetable oils and their importance in cosmetic industry. Chemik, 68(2), 103-110.


Abstract Views: 425

PDF Views: 3




  • In Vitro Culture-An Alternative Method for the Enhanced Production of Some Volatile Components of Ocimum Citriodorum Vis

Abstract Views: 425  |  PDF Views: 3

Authors

Anamika Tripathi
School of Sciences, Indira Gandhi National Open University, MaidanGarhi, New Delhi, India
N. S. Abbas
Department of Biology, BCAS, University of Delhi, New Delhi, India
Amrita Nigam
School of Sciences, Indira Gandhi National Open University, MaidanGarhi, New Delhi, India

Abstract


Ocimum plants are highly valued for their effective antibacterial, antifungal, antioxidant and therapeutic activities. These properties are attributed to some active metabolites. In order to enhance and develop an alternative source for the production of such active components, a comparative study was carried out. Composition of volatile organic compounds emitted by in vitro shoots, calli derived from leaf and nodal explant of Ocimum citriodorum Vis. were studied using Gas Chromatography-Mass Spectroscopy (GC-MS) and compared to those emitted by mother plants. Solvent extraction method was used for obtaining essential oils from the samples. Total 18 volatile compounds from the leaf extract of mother plant, 14 volatile compounds from the leaf extract of in vitro regenerated plants, 5 volatile compounds from the calli derived from leaf and 10 volatile compounds from the calli derived from nodal explant were identified.The content of nerol (2.44%) and geranylacetate (1.53%) was greater in in vitro regenerated plantlets than in comparison to mother plant (nerol- 0.88% and geranylacetate- 0.32%). Seven new compounds (isocaryophyllene, β-farnesene, β-himachalene, globulol, geranylformate, β-citronellylacetate, citronellylisobutyrate) were identified from the in vitro regenerated plants while six and three new compounds were identified from the calli derived from nodal explant (geraniol, trans-β-bergamotene, β-longipinene, bergamotene, geranylformate and β-citronellyacetate) and leaf (β-curcumene, trans-β-bergamotene, β-citronellol) respectively.From the present study, it can be concluded that in vitro propagated plants as well as calli derived from leaf and nodal segment may be used as an alternate source for the increased production of bioactive compounds.

Keywords


Ocimum Citriodorum Vis., GC-MS, Monoterpenes, Sesquiterpenes, Phenolic Terpenes, Terpene Esters.

References