Journal of Scientific and Technical Research (Sharda University, Noida)

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HPTLC-Densitometric Method for Estimation of in Bulk, Pharmaceutical Formulations


Affiliations
1 Central Revenues Control Laboratory, Pusa Campus, Delhi, India
2 Amity Institute of Biotechnology, Amity University, Gurgoan, Haryana, India
3 Department of Applied Chemistry and Polymer Technology, Delhi Technological University, Delhi, India
     

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A simple, sensitive and precise HPTLC (high performance thin layer chromatography) method has been developed for quantitative estimation of ketamine in bulk and pharmaceutical formulations. The separation of ketamine was achieved over HPTLC glass plate of silica GF- 254 by using cyclohexane:toluene:diethylamine (5:3:2 v/v) solvent system. Densitometric evaluation was carried out in the UV region at wavelength of 254. The method was found to be accurate (98.92-100.26%) and precise (CV = 0.43-0.67 for intra-day and CV = 0.58-1.45 for inter-day) within the corresponding linear range. The limit of detection (LOD) and limit of quantification (LOQ) of sample have been found to be 100 ng/spot and 303 ng/spot, respectively.

Keywords

Date Rape Drug, Densitometric Method, Drug of Abuse, General Anesthetic, Hallucinogen, HPTLC, Ketamine.
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  • HPTLC-Densitometric Method for Estimation of in Bulk, Pharmaceutical Formulations

Abstract Views: 294  |  PDF Views: 3

Authors

Aradhana Sharma
Central Revenues Control Laboratory, Pusa Campus, Delhi, India
Kumar Gaurav
Amity Institute of Biotechnology, Amity University, Gurgoan, Haryana, India
Ram Singh
Department of Applied Chemistry and Polymer Technology, Delhi Technological University, Delhi, India
Richa Srivastava
Department of Applied Chemistry and Polymer Technology, Delhi Technological University, Delhi, India

Abstract


A simple, sensitive and precise HPTLC (high performance thin layer chromatography) method has been developed for quantitative estimation of ketamine in bulk and pharmaceutical formulations. The separation of ketamine was achieved over HPTLC glass plate of silica GF- 254 by using cyclohexane:toluene:diethylamine (5:3:2 v/v) solvent system. Densitometric evaluation was carried out in the UV region at wavelength of 254. The method was found to be accurate (98.92-100.26%) and precise (CV = 0.43-0.67 for intra-day and CV = 0.58-1.45 for inter-day) within the corresponding linear range. The limit of detection (LOD) and limit of quantification (LOQ) of sample have been found to be 100 ng/spot and 303 ng/spot, respectively.

Keywords


Date Rape Drug, Densitometric Method, Drug of Abuse, General Anesthetic, Hallucinogen, HPTLC, Ketamine.