Journal of Scientific and Technical Research (Sharda University, Noida)

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Isolation, Identification and Antimicrobial Susceptibility of Shiga Toxin-Producing Escherichia Coli Serotypes in Chickens in Maiduguri, Nigeria


Affiliations
1 Department of Food Science and Technology, Ramat Polytechnic Maiduguri, Borno State, Nigeria
2 Department of Veterinary Microbiology, University of Maiduguri, Borno State, Nigeria
3 Department of Veterinary Medicine, University of Maiduguri, Borno State, Nigeria
4 Department of Microbiology, University of Maiduguri, Borno State, Nigeria
     

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This study was carried out in Maiduguri to isolate, characterize and determine the antimicrobial susceptibility of Shiga Toxin-producing Escherichia coli (STEC) in broilers and village chickens faeces. Conventional microbiology culture, phenotypical characterization, and biochemical test were used. Three hundred (300) (150 each of broiler and village chickens) faecal samples were randomly collected from chickens brought to the Maiduguri Monday Market Chicken slaughterhouse. A total of 83 (27.7%) E. coli isolates were obtained comprising 36(24%) from broiler chickens and 47(31.3%) from village chickens. The E.coli positive isolates were then serotyped based on their somatic ‘O’ antigen using latex agglutination test for O157 STEC and dry spot polyvalent Sero-check for Non-O157 STEC. Total of 19 (12.7%) and 26 (17.3%) were O157 STEC; while 8(5.3%) and 9(6%) were non-O157 STEC for both broilers and village chickens respectively. The remaining E. coli isolates 9(6%) from broiler chickens, and 12(8%) from village chickens were untypable using the conventional sero kits. The result of the antimicrobial sensitivity test using Clinical and Laboratory Standard Institute Guide revealed that the STEC isolates were susceptible to the antibiotics. With high susceptibility to Ciprofloxacin (100%), Ceftriaxone (98%) and Gentamicin (100%) and low susceptibility to Trimethoprim (79%), Chloramphenicol (66.6%) and resistant to cefodizime (73.1%) and Ampicillin (75%).

Keywords

Broiler Chickens, Village Chickens, Shiga Toxin-Producing E.Coli, Stx1, Stx2, O157, Non O157, Antimicrobial Susceptibility.
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  • D. W. K. Acheson, L. Lincicome, M. Jacewicz, and A. Kevisch, “Shiga toxin introduction with intestinal epithelial cells Escherichia coli O157: H7 and other Shiga toxin-producing E. coli strains,” Washington D.C. ASM Press, vol. 6, pp. 302-306, 1998.

  • A. M. Sakuma, “Prevalence and characterization of Shiga toxin-producing Escherichia coli sero groups from camels, cattle and humans in Maiduguri,” Unpublished PhD thesis, University of Maiduguri, Nigeria, pp. 103-112, 2014.

  • M. T. Askari, M. ZahaeiSalehi, K. H. Rabbani, G. Tadjbakhsh, N. Brujeni, and M. G. Nandalian, “Virulence genes profile and intimin subtypes of STEC isolated from healthy and diarrhoeic calves,” Veterinary Record, vol. 167, no. pp. 858-861, 2010. Doi: 10.1136/vr.c4009.

  • J. E. Blanco, M. Blanco, A. Mora, W. H. Jansen, V. Garcia, M. L. Vazquez, and J. Blanco, “Serotypes of Escherichia coli isolated from septicaemia chickens in Galicia (North-eastern Spain),” Veterinary Microbiology, vol. 61, no. 3, pp. 229-235, 1998.

  • R. J. F. Brando, E. Milivesbsky, L. Bentacor, N. Deza, A. Baschiuer, M. V. Ramos, G. C. Fernendez, R. Meiss, M. Rivas, M. S. Palermo, “Rural Damage and death in weaver mice after oral infection with Shiga toxin2-producing Escherichia coli strain,” Linvcal and Experimental Immunology, vol. 153, no. 2, pp. 297-306, 2008.

  • O. A. Daini, and A. Adesemowo, “Antimicrobial susceptibility patterns and R-plasmids of clinical strains of Escherichia coli,” Australian Journal of Basic and Applied Sciences, vol. 2, no. 3, pp. 397-400, 2008.

  • C. Farina, A. Goglio, G. Conedera, F. Minelli, and A. Caprioli, “Antimicrobial susceptibility of Escherichia coli O157 and other enterohaemorrhagic Escherichia coli isolated in Italy,” European Journal of Clinical Microbiology and Infectious Diseases, vol. 15, no. 4, pp. 351-353, 1996.

  • J. C. Galland, D. R. Hyatt, S. S. Crupper, and D. W. Acheson, “Prevalence, antibiotic susceptibility, and diversity of Escherichia coli O157: H7 isolates from a longitudinal study of beef cattle feedlots,” Applied and Environmental Microbiology, vol. 67, no. 4, pp. 1619-1627, 2001.

  • A. M. Gisilambe, “An aspect of pathological and bio metrology of semi-and tropical environment,” A case study of Maiduguri, Borno State. M.Sc. Dissertation, University of Ibadan, Nigeria, pp. 14-30, 1990.

  • L. H. Gould, “Update on the epidemiology of Shiga toxin-producing E. coli in the United States,” In Annual Capital Area Food Protection Association Meeting, Washington, DC. 2009.

  • P. M. Griffin, and G. B. Thomas, “Escherichia coli O157: H7,” In Emerging infections, American Society of Microbiology, vol. 1, pp. 137-146, 1998.

  • C. L. Gyles, and J. M. Fairbrother, Escherichia coli. In pathogenesis of bacterial infection in animals, (3rded.). pp. 193-223, 2004.

  • C. L. Gyles, “Shiga toxin-producing Escherichia coli: An over view,” Journal of Animal Science, vol. 85, pp. 13, pp. 45-62, 2006.

  • R. S. Hendriksen, Laboratory protocols level 2 training course susceptibility testing of salmonella using disk diffusion. WHO-Global Sam surveillance, (3rded.), 2003.

  • M. A. Islam, A. S. Mondol, E. D. Boer, R. R. Beumer, M. H. Zwietering, K. A. Talukder, and A. E. Heuvelink, “Prevalence and genetic characterization of shiga toxin-producing Escherichia coli isolates from slaughtered animals in Bangladesh,” Applied and Environmental Microbiology, vol. 74, no. 17, pp. 5414-5421, 2008.

  • M. A. Karmali, M. Mascarenhas, S. Shen, K. Ziebell, S. Johnson, R. R. Smith, J. I. Renton, C. Clark, K. Rahn, and J. B. Kaper,” Association of genomic O island 122 of Escherichia coli EDL 933 with verocytotoxin-producing Escherichia coli seropathotypes that are linked to epidemic and/or serious disease.” Journal of Clinical Microbiology, vol. 41, no. 11, 4930-4940, 2003.

  • M. A. Karmali, V. Gannon, and J. M. Sargeant, “Verocytotoxin-producing Escherichia coli,” Veterinary Microbiology, vol. 140, no. 3-4, pp. 360-370, 2010.

  • H. Kobayashi, P. Tarja, and P. Sinikka, “Prevalence and characteristics of Intiman-and Shiga toxin-producing Escherichia coli from gulls, pigeons and broilers in Finland,” Journal of Veterinary Medical Science, vol. 64, no. 11, pp. 1071-1073, 2002.

  • S. I. Makino, H. Kobori, H. Asakura, M. Watarai, T. Shirahata, T. Ikeda, K. Takeshi, and T. Tsukamoto, “Detection and characterization of Shiga toxin-producing Escherichia coli from seagulls.” Epidemiology & Infection, vol. 125, no. 1, pp. 55-61, 2000.

  • A. E. Moses, Epidemiological studies on some enteropathogenic E .coli serotypes in Borno and Adamawa States, Nigeria. PhD Thesis, Department of Veterinary Microbiology and Parasitology, University of Maiduguri, Nigeria, pp. 87-93, 2005.

  • Moses, A. E., J. A. Ameh, and G. O. Egwu. “Prevalence of Escherichia coli serogroups O157, O118, O111 and O26, in cattle faeces, dairy products and water samples in Borno and Adamawa States of Nigeria,” In Proceedings of the 42nd Annual Congress of Nigeria Veterinary Medical Association, pp. 14-18. 2005.

  • B. James, and A. D. O’Brien, “Escherichia coli 0157: H7 and other Shiga toxin-producing E. coli strains,” ASM Press, 1998.

  • I. N. Okeke, O. Ojo, A. Lamikanra, and J. B. Kaper, “Etiology of acute diarrhoea in adults in south-western Nigeria,” Journal of Clinical Microbiology, vol. 41, no. 10, pp. 4525-4530, 2003.

  • I. D. Olorunshola, S. I. Smith, and A. O. Coker, “Prevalence of EHEC O157: H7 in patients with diarrhoea in Lagos, Nigeria,” APMIS: Journal of Pathology, Microbiology and Immunology, vol. 108, no. 11, pp. 761-763, 2000.

  • A. Paton, and J. Paton, Detection and characterization of Shiga toxin toxigenic Escherichia coli by using multiplex PCR assay for stx2 eave A enterohaemorrhagic E. colittly Art b O111 and RFB O157,” Journal of Clinical Microbiology, vol. 36, no. 2, pp. 598-602, 1998.

  • S. Persson, K. E. P. Olsen, S. Ethelberg, and F. Scheutz, “Sub-typing method for Escherichia coli Shiga toxin (verocytotoxin) 2 variants and correlations to clinical manifestations,” Journal of Clinical Microbiology, vol. 45, no. 6, pp. 2020-2024, 2007.

  • Radostits, O. M., and C. C. Gay. “Traumatic reticuloperitonitis In: Radostits, OM, Gay, CC, Hinchcliff, KW, Constable, PD Eds.” Veterinary Medicine: A Textbook of the Diseases of Cattle, Horses, Sheep, Pigs and Goats, (10thed.). Elsevier Health Sciences, Philadelphia, PA, USA, pp. 337-352, 2007.

  • E. Rahimi, H. Momtaz, and N. Nozarpour, “Prevalence of Listeria spp., Campylobacter spp., and Escherichia coli O157: H7 isolated from camel carcasses during processing,” Bulgarian Journal of Veterinary Medicine, vol. 13, no. 3, pp. 179-185, 2010.

  • M. Raji, J. Adekeye, J. Kwaga, J. Bale, and M. Henton, “Serovars and biochemical characterization of Escherichia coli isolated from colibacillosis cases and dead-in-shell embryos in poultry in Zaria-Nigeria,” Veterinarski Arhiv, vol. 77, no. 6, pp. 495-505, 2007.

  • C. M. Schroeder, D. G. White, B. Ge, Y. Zhang, P. F. McDermott, S. Ayers, S. Zhao, and J. Meng, “Isolation of antimicrobial-resistant Escherichia coli from retail meats purchased in Greater Washington, DC, USA,” International Journal of Food Microbiology, vol. 85, no. 1-2, pp. 197-202, 2003.

  • Synge, B. A., and G. F. Hopkins. “Studies of verotoxigenic Escherichia coli O157 in cattle in Scotland and association with human cases,” Recent advances in verocytotoxin-producing Escherichia coli infections. Amsterdam: Elsevier Science, pp. 65-8, 1994.

  • L. B. Tekdek, J. O. Adekeye, E. O. Gyang, and J. U. Umoh, “Prevalence of Verotoxin-producing Escherichia Coli from raw milk and characterisation of E. Coli from Calves’ Faeces in Nigeria,” Bulletin of Animal Health and Production in Africa, vol. 43, no. 4, pp. 237-242, 1995.

  • L. H. Wieler, and R. Bauerfeind, “STEC as a Veterinary Problem,” In E. coli, pp. 75-89. Humana Press, 2003.

  • Y. A. Geidam, A. G. Ambali, and P. A. Onyeyili, “Detection and antibiotic sensitivity pattern of avian pathogenic Escherichia coli strains among rural chickens in the arid region of north-eastern Nigeria,” Veterinary World, vol. 5, no. 6, p. 325, 2012.


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  • Isolation, Identification and Antimicrobial Susceptibility of Shiga Toxin-Producing Escherichia Coli Serotypes in Chickens in Maiduguri, Nigeria

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Authors

Falmata A. Abadam
Department of Food Science and Technology, Ramat Polytechnic Maiduguri, Borno State, Nigeria
Dahiru El. Yuguda
Department of Veterinary Microbiology, University of Maiduguri, Borno State, Nigeria
Yagub A. Geidam
Department of Veterinary Medicine, University of Maiduguri, Borno State, Nigeria
Isa A. Gulani
Department of Veterinary Medicine, University of Maiduguri, Borno State, Nigeria
Lawan Adamu
Department of Veterinary Medicine, University of Maiduguri, Borno State, Nigeria
Mustafa Alhaji Isa
Department of Microbiology, University of Maiduguri, Borno State, Nigeria

Abstract


This study was carried out in Maiduguri to isolate, characterize and determine the antimicrobial susceptibility of Shiga Toxin-producing Escherichia coli (STEC) in broilers and village chickens faeces. Conventional microbiology culture, phenotypical characterization, and biochemical test were used. Three hundred (300) (150 each of broiler and village chickens) faecal samples were randomly collected from chickens brought to the Maiduguri Monday Market Chicken slaughterhouse. A total of 83 (27.7%) E. coli isolates were obtained comprising 36(24%) from broiler chickens and 47(31.3%) from village chickens. The E.coli positive isolates were then serotyped based on their somatic ‘O’ antigen using latex agglutination test for O157 STEC and dry spot polyvalent Sero-check for Non-O157 STEC. Total of 19 (12.7%) and 26 (17.3%) were O157 STEC; while 8(5.3%) and 9(6%) were non-O157 STEC for both broilers and village chickens respectively. The remaining E. coli isolates 9(6%) from broiler chickens, and 12(8%) from village chickens were untypable using the conventional sero kits. The result of the antimicrobial sensitivity test using Clinical and Laboratory Standard Institute Guide revealed that the STEC isolates were susceptible to the antibiotics. With high susceptibility to Ciprofloxacin (100%), Ceftriaxone (98%) and Gentamicin (100%) and low susceptibility to Trimethoprim (79%), Chloramphenicol (66.6%) and resistant to cefodizime (73.1%) and Ampicillin (75%).

Keywords


Broiler Chickens, Village Chickens, Shiga Toxin-Producing E.Coli, Stx1, Stx2, O157, Non O157, Antimicrobial Susceptibility.

References