Open Access Open Access  Restricted Access Subscription Access

Antimicrobial Potential of Green Synthesized Silver Nanoparticles Using Sida acuta Leaf Extract


Affiliations
1 School of Nano Sciences, Central University of Gujarat, Gandhinagar, Gujarat, India
2 School of Environment and Sustainable Development, Central University of Gujarat, Gandhinagar, Gujarat, India
 

Plants are the natural factories for nanoparticle production as many of their products are being used for metallic nanoparticles production. Silver nanoparticles are being used in a number of consumer products, remediation processes, and medicines due to their antimicrobial and anti-inflammatory and catalytic activities. Present work focuses on a simple, one-step, environmental-friendly biosynthesis of silver nanoparticles using silver nitrate as precursor and leaf extract of herb species (Sida acuta); a common wireweed of Malvaceae family, which acts as reducing as well as capping agent. Synthesized nanoparticles were characterized for their morphological description using different techniques like UV-Vis spectroscopy, dynamic light scattering (DLS), transmission electron microscope (TEM) and Fourier transform infra-red (FT-IR) spectroscopy. The antimicrobial activity of these nanoparticles was studied against Pseudomonas aeruginosa and Candida albicans. The results showed good inhibitory effect against Pseudomonas aeruginosa and Candida albicans and found to exhibit good antibacterial activity especially at lower concentrations of 4 μg/ml and 8 μg/ml.

Keywords

Silver Nanoparticles, Leaf Extract, Antifungal Effect, Antibacterial Activity.
User
Notifications
Font Size

  • Akhtar MS, Panwar J, Yun YS. Biogenic synthesis of metallic nanoparticles by plant extracts. ACS Sust Chem Eng. 2013;1:591-602.
  • Hebbalalu D, Lalley J, Nadagouda MN, et al. Greener techniques for the synthesis of silver nanoparticles using plant extracts, enzymes, bacteria, biodegradable polymers, and Microwaves. ACS Sust Chem Eng. 2013;1:703-12
  • Forough M, Farhadi K. Biological and green synthesis of silver nanoparticles. Turkish J Eng Env Sci. 2010;34:281-7.
  • Iravani S, Zolfaghari B. Green synthesis of silver nanoparticles using Pinus eldarica bark extract. Hindawi Publishing Corporation. BioMed Res Inter. 2013:5.
  • Renugadevi K, Aswini RV. Microwave irradiation assisted synthesis of silver nanoparticles using Azadirachta indica leaf extract as a reducing agent and in vitro evaluation of its antibacterial and anticancer activity. Int J Nanomater Biostruct. 2012;2(2):5-10.
  • Bankar A, Joshi B, Kumar AR, et al. Banana peel extract mediated novel route for the synthesis of silver nanoparticles. Colloids Surf A. Physicochem Eng Asp. 2010;368:58-63.
  • Ghosh S, Patil S, Ahire M, et al. Synthesis of silver nanoparticles using Dioscorea bulbifera tuber extract and evaluation of its synergistic potential in combination with antimicrobial agents. Int J Nanomed. 2012;7:483-96.
  • Bar H, Bhui DK, Gobinda SP. Green synthesis of silver nanoparticles using seed extract of Jatropha curcas. Physicochem
  • Jain D, Daima HK, Kachhwaha S, et al. Synthesis of plant-mediated silver nanoparticles using papaya fruit extract and evaluation of their antimicrobial activities. Dig J Nanomat Bios. 2009;4:557-63.
  • Eghdami A, Sadeghi F. Determination of total phenolic and flavonoids contents in methanolic and aqueous extract of Achillea millefolium. Org Chem J. 2010:2:81-4.
  • Cowan MM. Plant products as antimicrobial agents”. Clin Microbiol Rev. 1999;12(4):564-82.
  • Geethalakshmi R, Sarada DVL. Synthesis of plant-mediated silver nanoparticles using Trianthema decandra extract and evaluation of their antimicrobial activities. Int J Eng Sci Tech. 2010; 2(5):970-5.
  • Sivakumar P, Lavanya R, Priya MV, et al. Synthesis of MgO/Ag nanocomposite with enhanced photocatalytic activity against textile dye. J Chem Pharma Sci. 2014;S(4):119-20.
  • Chang LT, Yen CC. Studies on the preparation and properties of conductive polymers. VIII. Use of heat treatment to prepare metalized films from silver chelate of PVA and PAN. J Appl Polym Sci. 1995;55:371-374.
  • Ganeshkumar M, Sathishkumar M, Ponrasu T, et al. Spontaneous ultra-fast synthesis of gold nanoparticles using Punica granatum for cancer targeted drug delivery. Colloid Surface B. 2013;106:208-16.
  • Chandran SP, Chaudhary M, Pasricha R, et al. Synthesis of gold nanotriangles and silver nanoparticles using Aloe vera plant extract. Biotechnol Prog. 2006;22(2):577-83.
  • Lalitha A, Subbaiya R, Ponmurugan P. Green synthesis of silver nanoparticles from leaf extract Azhadirachta indica and to study its anti-bacterial and antioxidant property. Int J Curr Microbiol App Sci. 2013;2(3):228-35.
  • Kim KJ, Sung WS, Suh BK, et al. Antifungal activity and mode of action of silver nano-particles on Candida albicans. Biometals. 2009;22(2):235-42.
  • Rogers JV, Parkinson CV, Choi YW, et al. A preliminary assessment of silver nanoparticle inhibition of monkeypox virus plaque formation. Nanoscale Res Lett. 2008;3(4):129-33.
  • Gurunathan S,Lee KJ, Kalishwaralal K, et al. Antiangiogenic properties of silver nanoparticles. Biomaterials. 2009;30(31):6341-50.
  • Nadworny PL, Wang J, Tredget EE, et al. Anti-inflammatory activity of nanocrystalline silver in a porcine contact dermatitis model. Nanomedicine 2008;4(3):241-251.
  • Greßler S, Gazso A. Nanotechnology in cosmetics. Nano Dossiers. 2010:008.
  • Satyavani K, Gurudeeban S, Ramanathan T, e al. Biomedical potential of silver nanoparticles synthesized from calli cells of Citrullus colocynthis (L.) Schrad. J Nanobiotechnol. 2011;9:43.
  • Prabhu S, Poulose EK. Silver nanoparticles: Mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects. International Nano Letters.2012;2:32.
  • Guzman MG, Dille J, Godet S. Synthesis of silver nanoparticles by chemical reduction method and their antibacterial activity. Int J Chem Biolog Eng. 2009;2:3.
  • Phatak RS, Hendre AS. Sunlight induced green synthesis of silver nanoparticles using sundried leaves extract of Kalanchoe pinnata and evaluation of its photocatalytic potential. Der Pharmacia Lett. 2015;7(5):313-24.
  • Zaarour M, Mohamad ER, Biao D, et al. Photochemical preparation of silver nanoparticles supported on Zeolite crystals. Langmuir. 2014;30(21):625-6.
  • Darroudi M, Zak AK, Muhamad MR, et al. Green synthesis of colloidal silver nanoparticles by sonochemical method. Mater Lett. 2012;66(1):117-20.
  • Kumar RM, Rao BL, Asha S, et al. Gamma radiation assisted biosynthesis of silver nanoparticles and their characterization. Adv Mater Lett. 2015;6(12):1088-93.
  • Wang B, Zhuang X, Deng W, et al. Microwave-assisted synthesis of silver nanoparticles in alkalic carboxymethyl Chitosan solution. Eng. 2010;2:387-90.
  • Trivedi HB, Vediya SD. Removal of fluoride from drinking water with the help of Sida acuta Burm f. Int J pharm life sci. 2013;4(8).
  • Mulvaney P. Surface plasmon spectroscopy of nanosized metal particles. Langmuir. 1996;12(3):788-800.
  • Berne BJ, Pecora R. Dynamic Light Scattering: With Applications to Chemistry, Biology, and Physics, Dover, New York, NY.USA. 2000.
  • Jorgensen JH, Turnidge JD. Manual of clinical Microbiology. 11th ed. United States: American Society for Microbiology; 2015.71, Susceptibility test methods: Dilution and disk diffusion methods; 1152-73.
  • Ingroff E, Pfaller MA. Manual of clinical microbiology. 10th ed. United States: American Society for Microbiology; 2007. Susceptibility test methods: Yeasts and filamentous fungi; 1972-86.
  • Li Q, Mahendra S, Lyon DY, et al. Antimicrobial nanomaterials for water disinfection and microbial control: Potential applications and implications. Water Research 2008;42:4591-2.
  • Nasrollahi A, Pourshamsian Kh, Mansourkiaee P. Antifungal activity of silver nanoparticles on some of fungi. Int J Nano Dimension. 2011;1(3):233-9.
  • Khushboo S, Manju P, Sangeeta K, et al. Antibacterial Activity of Synthesized Silver Nanoparticles from Tinospora cordifolia against Multi Drug Resistant Strains of Pseudomonas aeruginosa Isolated from Burn Patients. J Nanomed Nanotechnol. 2014;5(2):2157-7439
  • Velusamy P, Venkat KG, Jeyanthi V, et al. Bio-Inspired Green Nanoparticles: Synthesis, Mechanism, and Antibacterial Application. Toxicol Res. 2016;32(2):95-102.
  • Ramamurthy CH, Padma M, Samadanam ID, et al. The extra cellular synthesis of gold and silver nanoparticles and their free radical scavenging and antibacterial properties. Colloids Surf B Biointerfaces. 2013;102:808-15.

Abstract Views: 321

PDF Views: 4




  • Antimicrobial Potential of Green Synthesized Silver Nanoparticles Using Sida acuta Leaf Extract

Abstract Views: 321  |  PDF Views: 4

Authors

C. Nisha
School of Nano Sciences, Central University of Gujarat, Gandhinagar, Gujarat, India
P. Bhawana
School of Environment and Sustainable Development, Central University of Gujarat, Gandhinagar, Gujarat, India
M. H. Fulekar
School of Environment and Sustainable Development, Central University of Gujarat, Gandhinagar, Gujarat, India

Abstract


Plants are the natural factories for nanoparticle production as many of their products are being used for metallic nanoparticles production. Silver nanoparticles are being used in a number of consumer products, remediation processes, and medicines due to their antimicrobial and anti-inflammatory and catalytic activities. Present work focuses on a simple, one-step, environmental-friendly biosynthesis of silver nanoparticles using silver nitrate as precursor and leaf extract of herb species (Sida acuta); a common wireweed of Malvaceae family, which acts as reducing as well as capping agent. Synthesized nanoparticles were characterized for their morphological description using different techniques like UV-Vis spectroscopy, dynamic light scattering (DLS), transmission electron microscope (TEM) and Fourier transform infra-red (FT-IR) spectroscopy. The antimicrobial activity of these nanoparticles was studied against Pseudomonas aeruginosa and Candida albicans. The results showed good inhibitory effect against Pseudomonas aeruginosa and Candida albicans and found to exhibit good antibacterial activity especially at lower concentrations of 4 μg/ml and 8 μg/ml.

Keywords


Silver Nanoparticles, Leaf Extract, Antifungal Effect, Antibacterial Activity.

References