Nano Science & Nano Technology: An Indian Journal

Open Access Open Access  Restricted Access Subscription Access

The Release Property of Amoxicillin Nanoparticle and their Antibacterial Activity


Affiliations
1 Nanomedicine-Laboratory of Immunology and Biomedical Research, School of Medicine, Deakin University, 75 Pigdons Rd Geelong, Australia
2 Biotechnology Department, College of Science, Al Nahrain University, Baghdad, Iraq
3 College of Pharmacy, Al Mustansyria University, Baghdad, Iraq
 

Early efforts have been made in an attempt to reduce the adverse side effect of multiple drug resistance organisms, and new classes of antimicrobial nanoparticles (NPs) and nanosized carriers for antibiotics delivery were developed. PURPOSE: This study was focused on the assessment of the physiochemical characterization, In vitro drug release, biofilm formation and antimicrobial properties of amoxicillin encapsulated within the Poly (ε-caprolactone) (PCL) nanoparticles. Methods: amoxicillin (AMX) nanoparticles were prepared using the emulsion solvent evaporation method with different concentrations of polycaprolactone (PCL) and Poly Vinyl Alcohol (PVA). These nanoparticles were subsequently characterized and evaluated for their antibacterial activity and biofilm inhibition activity using mean and standard error for Data analysis. Results: It was found that increased PCL concentration resulted in an increase in entrapment efficiency (EE%) to 83.3%. Meanwhile, an increase in the PVA concentration led to a decrease in the EE% and an increase in nanoparticle size. Enhancements in the percentage of practical yield to 80.2% as the polymer concentration rose. Fourier transform infrared spectra data for the MNPs, CS-coated AMX, and AMX-PCL-NPs nanoparticles were compared, which confirmed the PCL coating on the AMX and the AMX-PCL-NPs loaded nanoparticles. In addition, the antimicrobial activity of the nanoparticles e was determined using agar diffusion and growth inhibition assays against both gram-positive Staphylococcus aureus, gram-negative Pseudomonas aeruginosa and Proteus mirabilis bacteria. Furthermore, 10 μg/ml was the minimum inhibitory concentration of the AMX-PCL-NPs nanoparticle which inhibited biofilm formation in Staphylococcus aureus bacteria. Conclusion: Thus, this study presents a novel ß-lactam antibacterial-nanocarrier system that can reduce and inhibit bacterial growth showing it to be a promising tool for numerous medical applications.

Keywords

Poly (ε-caprolactone), Poly Vinyl Alcohol, Amoxicillin Nanoparticle, Antimicrobial Activity, Biofilm.
User
Notifications
Font Size

  • Liu H, Leonas KK, Zhao Y. Antimicrobial properties and release profile of ampicillin from electrospun poly (ε-caprolactone) nanofiber yarns. J Eng Fiber Fabr. 2010;5(4):0-19.

  • Patel VR, Amiji MM. Preparation and characterization of freeze-dried chitosan-poly (ethylene oxide) hydrogels for site-specific antibiotic delivery in the stomach. Pharm Res. 1996;13(4):588-93.

  • Chen C. Bioadhesive polymers for stomach targeted drug delivery. Proceedings of the 24th International Symposium on Controlled Release of Bioactive Materials. Stockholm. 1997.

  • Park H, Park k, Shalaby WS. Biodegradable hydrogels fBor drug delivery. 2011.

  • Sahoo SK, Labhasetwar V. Enhanced antiproliferative activity of transferrin-conjugated paclitaxel-loaded nanoparticles is mediated via sustained intracellular drug retention. Mol Pharm. 2005;2(5):373-83.

  • Bernardi A. Selective cytotoxicity of indomethacin and indomethacin ethyl ester-loaded nanocapsules against glioma cell lines: an in vitro study. Eur J Pharmacol. 2008;586(1-3):24-34.

  • Hillaireau H, Couvreur P. Nanocarriers’ entry into the cell: Relevance to drug delivery. Cell Mol Life Sci. 2009;66(17):2873-96.

  • Güncüm E, Işıklan N, Anlaş C, et al. Development and characterization of polymeric-based nanoparticles for sustained release of the amoxicillin-an antimicrobial drug. Artif Cells Nanomed Biotechnol. 2018;46:964-73.

  • Mora-Huertas CE, Fessi H, Elaissari A, Polymer-based nanocapsules for drug delivery. Int J Pharm. 2010;385(1-2):113-42.

  • Niwa T, Takeuchi H, Hino T, et al. In vitro drug release behavior of D, L‐lactide/glycolide copolymer (PLGA) nanospheres with nafarelin acetate prepared by a novel spontaneous emulsification solvent diffusion method. J Pharm Sci. 1994;83(5):727-32.

  • Ramteke S, Maheshwari RB, Jain NK. Clarithromycin based oral sustained release nanoparticulate drug delivery system. Indian J Pharm Sci. 2006;68(4):479-84.

  • El-Shabouri MH. Positively charged nanoparticles for improving the oral bioavailability of cyclosporin-A. Int J Pharm. 2002;249(1-2):101-08.

  • Yeo Y, Park k. Control of encapsulation efficiency and initial burst in polymeric microparticle systems. Arch Pharm Res. 2004;27(1):1-12.

  • Atlas RM, Brown AE, Parks LC. Laboratory manual of experimental microbiology. 1995.

  • Jumaa T, Chasib M, Hamid MK, et al., Effect of the electric field on the antibacterial activity of Au nanoparticles on some Gram-positive and Gram-negative bacteria. Nanosci Nanotech Res. 2014;2(1):1-7.

  • Al-Moahmmedawi MC. Nanoformulation and antibiotic releasing property of cefotaxime nanoparticles.African J Biotech. 2016;15(14):539-48.

  • Wiegand I, Hilpert K, Hancock RE. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat Protoc. 2008;3(2):163-75.

  • Sahoo SK, Panyam J, Prabha S, et al. Residual polyvinyl alcohol associated with poly (D, L-lactide-co-glycolide) nanoparticles affects their physical properties and cellular uptake. J Control Release. 2002;82(1):105-14.

  • Ranjha NM, Khan IU, Naseem S. Encapsulation and characterization of flurbiprofen loaded poly (є-caprolactone)-poly (vinylpyrrolidone) blend microspheres by a solvent evaporation method. J Sol-Gel Sci Tech. 2009;50(3):281-89.

  • Abdelmalek I, Svahn I, Mesli S, et al. Ormulation, evaluation and microbiological activity of ampicillin and amoxicillin microspheres. J Mater Environ Sci. 2014;5(6):1799-1807.

  • von Burkersroda F, Schedl L, Göpferich A. Why degradable polymers undergo surface erosion or bulk erosion. Biomaterials. 2002;23(21):4221-31.

  • Characklis WG. Bioengineering report: Fouling biofilm development: A process analysis. Biotechnol Bioeng. 1981;23(9):1923-60.

  • Bowen WR, Doneva TA. Atomic force microscopy studies of membranes: Effect of surface roughness on double-layer interactions and particle adhesion. J Colloid Interface Sci. 2000;229(2):544-49.

  • Hans ML, Lowman AM. Biodegradable nanoparticles for drug delivery and targeting. Curr Opin Solid St M. 2002;6(4):319-27.

  • Joshy MA, Elayaraja K, Suganthi RV, et al. in vitro sustained release of amoxicillin from lanthanum hydroxyapatite nano rods. Curr Appl Phys. 2011;11(4):1100-6.

  • Nguyen TV. Preparation of chitosan nanoparticles by TPP ionic gelation combined with spray drying, and the antibacterial activity of chitosan nanoparticles and a chitosan nanoparticle-amoxicillin complex. Res Chem Intermed. 2017;43(6):3527-37.

  • Ayala-Núñez NV, Villegas HH, Turrent L, et al. Silver nanoparticles toxicity and bactericidal effect against methicillin-resistant Staphylococcus aureus: Nanoscale does matter. Nano Biotechnol. 2009;5(1-4):2-9.

  • Srivastava N, Mukhopadhyay M. Green synthesis and structural characterization of selenium nanoparticles and assessment of their antimicrobial property. Bioproc Biosyst Eng. 2015;38(9):1723-30.

  • Rahim K, Mohamed AM. Bactericidal and antibiotic synergistic effect of nanosilver against methicillin-resistant Staphylococcus aureus. Jundishapur J Microbiol. 2015;8(11).


Abstract Views: 167

PDF Views: 0




  • The Release Property of Amoxicillin Nanoparticle and their Antibacterial Activity

Abstract Views: 167  |  PDF Views: 0

Authors

Maysaa Ch. AL-Mohammedawi
Nanomedicine-Laboratory of Immunology and Biomedical Research, School of Medicine, Deakin University, 75 Pigdons Rd Geelong, Australia
Rana A. Mohsien
Biotechnology Department, College of Science, Al Nahrain University, Baghdad, Iraq
Mokhtar J. Kadhim
Biotechnology Department, College of Science, Al Nahrain University, Baghdad, Iraq
Enas S. Bahjat
Biotechnology Department, College of Science, Al Nahrain University, Baghdad, Iraq
Duna Q. Al Azawi
Biotechnology Department, College of Science, Al Nahrain University, Baghdad, Iraq
Samra A. Qaraghuli
College of Pharmacy, Al Mustansyria University, Baghdad, Iraq

Abstract


Early efforts have been made in an attempt to reduce the adverse side effect of multiple drug resistance organisms, and new classes of antimicrobial nanoparticles (NPs) and nanosized carriers for antibiotics delivery were developed. PURPOSE: This study was focused on the assessment of the physiochemical characterization, In vitro drug release, biofilm formation and antimicrobial properties of amoxicillin encapsulated within the Poly (ε-caprolactone) (PCL) nanoparticles. Methods: amoxicillin (AMX) nanoparticles were prepared using the emulsion solvent evaporation method with different concentrations of polycaprolactone (PCL) and Poly Vinyl Alcohol (PVA). These nanoparticles were subsequently characterized and evaluated for their antibacterial activity and biofilm inhibition activity using mean and standard error for Data analysis. Results: It was found that increased PCL concentration resulted in an increase in entrapment efficiency (EE%) to 83.3%. Meanwhile, an increase in the PVA concentration led to a decrease in the EE% and an increase in nanoparticle size. Enhancements in the percentage of practical yield to 80.2% as the polymer concentration rose. Fourier transform infrared spectra data for the MNPs, CS-coated AMX, and AMX-PCL-NPs nanoparticles were compared, which confirmed the PCL coating on the AMX and the AMX-PCL-NPs loaded nanoparticles. In addition, the antimicrobial activity of the nanoparticles e was determined using agar diffusion and growth inhibition assays against both gram-positive Staphylococcus aureus, gram-negative Pseudomonas aeruginosa and Proteus mirabilis bacteria. Furthermore, 10 μg/ml was the minimum inhibitory concentration of the AMX-PCL-NPs nanoparticle which inhibited biofilm formation in Staphylococcus aureus bacteria. Conclusion: Thus, this study presents a novel ß-lactam antibacterial-nanocarrier system that can reduce and inhibit bacterial growth showing it to be a promising tool for numerous medical applications.

Keywords


Poly (ε-caprolactone), Poly Vinyl Alcohol, Amoxicillin Nanoparticle, Antimicrobial Activity, Biofilm.

References