Biosynthesis, Characterization, Nematicidal Efficacy of Silver Nanoparticles Synthesized using Solanum nigrum Fruit against Root Knot Nematode Meloidogyne incognita
The present study to synthesis Sliver nanoparticles by using fruit extract of European black nightshade (Solanum nigrum) and to test their characterized along with their potentiality to control the Meloidogyne incognita at different concentration. The synthesized AgNPs were characterized by UV-Vis Spectroscopy, Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction analysis (XRD). The synthesized AgNPs result of UV-Spectroscopy and the strong broad peak located at 443 nm. In SEM analysis, the synthesized silver nanoparticles was clearly seen that in a spherical shape. The size of AgNPs range is 30 nm. In FTIR analysis, IR bands of synthesised AgNPs observed functional groups are methyl, methylene and methoxy groups, secondary amines and vinyl groups. In XRD analysis, the average size of AgNPs particles is 29 nm and are crystalline in nature. In nematicidal activity of AgNPs on M. incognita at different concentrations the percent mortality caused by 2.5 µg/ml (100%) of AgNPs was higher than the percent mortality caused by 0.5 µg/ml (48%) of AgNPs.
Keywords
- Sasser JN. Root-knot nematodes: A global menace to crop production. Plant Dis. 1980;64(1):36-41.
- Oka Y, Ben‐Daniel B, Cohen Y. Nematicidal activity of the leaf powder and extracts of Myrtus communis against the root‐knot nematode Meloidogyne javanica. Plant Pathol. 2012 ;61(6):1012-20.
- Collange B, Navarrete M, Peyre G, et al. Root-knot nematode (Meloidogyne) management in vegetable crop production: The challenge of an agronomic system analysis. Crop Protect. 2011;30(10):1251-62.
- Bailey DJ, Kleczkowski A, Gilligan CA. Epidemiological dynamics and the efficiency of biological control of soil‐borne disease during consecutive epidemics in a controlled environment. New Phytol. 2004;161(2):569-75.
- Karpouzas DG, Karanasios E, Menkissoglu-Spiroudi U. Enhanced microbial degradation of cadusafos in soils from potato monoculture: Demonstration and characterization. Chemosphere. 2004;56(6):549-59.
- Qiu L, Liu F, Zhao L, et al. Evidence of a unique electron donor-acceptor property for platinum nanoparticles as studied by XPS. Langmuir. 2006;22(10):4480-2.
- Pyrowolakis A, Westphal A, Sikora RA, et al. Identification of root-knot nematode suppressive soils. Appl Soil Ecol. 2002;19(1):51-6. For this reason, AgNPs is a broad www.tsijournals.com | July-202
- Desselberger U. Emerging and re-emerging infectious diseases. J Infect. 2000;40(1):3-15.
- Park Y, Hong YN, Weyers A, et al. Polysaccharides and phytochemicals: A natural reservoir for the green synthesis of gold and silver nanoparticles. IET Nanobiotechnol. 2011;5(3):69-78.
- Sun Q, Cai X, Li J, et al. Green synthesis of silver nanoparticles using tea leaf extract and evaluation of their stability and antibacterial activity. Colloid Surfaces A: Physicochem Eng Aspects. 2014;444:226-31.
- Sun S, Murray CB, Weller D, et al. Monodisperse FePt nanoparticles and ferromagnetic FePt nanocrystal superlattices. Science. 2000;287(5460):1989-92.
- Ahamed M, Posgai R, Gorey TJ, et al. Silver nanoparticles induced heat shock protein 70, oxidative stress and apoptosis in Drosophila melanogaster. Toxicol Appl Pharmacol. 2010;242(3):263-9.
- Kulkarni N, Muddapur U. Biosynthesis of metal nanoparticles: A review. J Nanotechnol. 2014;2014.
- Kalishwaralal K, BarathManiKanth S, Pandian SR, et al. Silver nanoparticles impede the biofilm formation by Pseudomonas aeruginosa and Staphylococcus epidermidis. Colloid Surf B: Biointerfaces. 2010;79(2):340-4.
- Miraj S. Solanum nigrum: A review study with anti-cancer and antitumor perspective. Der Pharm Chem. 2016;8(17):62- 8.
- Chimentao RJ, Kirm I, Medina F, et al. Different morphologies of silver nanoparticles as catalysts for the selective oxidation of styrene in the gas phase. Chem Communicat. 2004(7):846-7.
- Choi Y, Ho NH, Tung CH. Sensing phosphatase activity by using gold nanoparticles. Angew Chem Int Ed Engl. 2007;119(5):721-3.
- He B, Tan JJ, Liew KY, et al. Synthesis of size controlled Ag nanoparticles. J Mole Cataly A: Chem. 2004;221(1-2):121- 6.
- Hutter E, Fendler JH. Exploitation of localized surface plasmon resonance. Adv Mater. 2004;16(19):1685-706.
- Zhang W, Qiao X, Chen J, et al. Preparation of silver nanoparticles in water-in-oil AOT reverse micelles. J Colloid Interface Sci. 2006;302(1):370-3.
- Bailey DJ, Kleczkowski A, Gilligan CA. Epidemiological dynamics and the efficiency of biological control of soil‐borne disease during consecutive epidemics in a controlled environment. New Phytol. 2004;161(2):569-75.
- Kelly KL, Coronado E, Zhao LL, et al. The optical properties of metal nanoparticles: The influence of size, shape, and dielectric environment. J Phys Chem B. 2003;107(3):668-77.
- Morones JR, Elechiguerra JL, Camacho A, et al. The bactericidal effect of silver nanoparticles. Nanotechnology. 2005;16(10):2346.
- Scharf A, Piechulek A, von Mikecz A. Effect of nanoparticles on the biochemical and behavioral aging phenotype of the nematode Caenorhabditis elegans. ACS Nano. 2013;7(12):10695-703.
- Meyer JN, Lord CA, Yang XY, et al. Intracellular uptake and associated toxicity of silver nanoparticles in Caenorhabditis elegans. Aquatic Toxicol. 2010;100(2):140-50.
- Ellegaard-Jensen L, Jensen KA, Johansen A. Nano-silver induces dose-response effects on the nematode Caenorhabditis elegans. Ecotoxicol Environ Safety. 2012;80:216-23.
- Cromwell WA, Yang J, Starr JL, et al. Nematicidal effects of silver nanoparticles on root-knot nematode in bermudagrass. J Nematol. 2014;46(3):261.
- Lim D, Roh JY, Eom HJ, et al. Oxidative stress‐related PMK‐1 P38 MAPK activation as a mechanism for the toxicity of silver nanoparticles to reproduction in the nematode Caenorhabditis elegans. Environ Toxicol Chem. 2012;31(3):585-92.
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