Research Journal of Engineering and Technology

M. Seenuvasan ¹, J. Sebastin ¹, K. Ananthi ¹, S.C. Gayathri ¹, A. Krithika Siva ¹, M. Anil Kumar ¹, K. Sathish Kumar ²

Affiliations
1 Department of Biotechnology, Madha Engineering College, Chennai, IN
2 Department of Chemical Engineering, SSN College of Engineering, Chennai, IN

Abstract

A sequential anode-cathode double-chamber microbial fuel cell (MFC) was constructed for the production of electricity using a novel microorganism, Clostridium sporogenes NCIM 5125 which is profoundly known to be a part of MFC studies. The growth of the microorganism was supported by energy derived from the electron transfer process itself and results in stable, long term power production. The electricity generation was optimized by analyzing the growth of the microbe at different parameters using mediated and non-mediated MFC. The results suggested that it is feasible to generate more electricity amounting to 5.6 mA at 160th h with the adopted C. sporogenes using mediated MFC.

Keywords

Microbial Fuel Cell, Proton Exchange Membrane, Clostridium sporogenes.

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S. Abinandan ¹, P. Pravin Kumar ², M. Anil Kumar ², M. Seenuvasan ²

Affiliations
1 School of Mechanical and Building Sciences, VIT University, Vellore, IN
2 Department of Biotechnology, Madha Engineering College, Kundrathur, Chennai, IN

Abstract

In this study, the microalgae Chlorella pyrenoidosa was subjected for growth with respect to supply different heterotrophic carbon sources such as glucose, sucrose and fructose, also autotrophic condition with CO₂ as the carbon source are considered in various concentrations such as 0%, 10% and 20%. The maximum biomass concentration of 7.3 g/L was obtained with glucose as the carbon source and the lipid productivity was said to be higher with 20% of CO₂ concentration with lipid content of 22.13%. This study concludes that the lipid content in the algal cells was increased at 20% of CO₂ as the carbon source. This indicates that the lipid content can be increased at the autotrophic growth condition mediated by the supply of CO₂ to the medium as the carbon source compared with that of the other heterotrophic level of carbon supplementary. The optimization of the algal growth has been performed at different growth conditions along with that of the different stress parameters together in order to understand and monitor the relation between the cell components of algae such as lipid content, protein content and chlorophyll content and their effect with that of the applied stress conditions.

Keywords

Biomass, Chlorella pyrenoidosa, Lipids, Yield.

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J. Niranjan ¹, Fazal Basha ¹, G. Suganya ¹, M. Anil Kumar ¹, M. Seenuvasan ¹, S. Selvanaveen ¹

Affiliations
1 Department of Biotechnology, Madha Engineering College, Chennai, IN

Abstract

The industrially used biopolymer levan as an emulsifying agent was produced in large amounts by optimizing the physical parameters like pH, temperature and incubation time, with the central composite design under the response surface methodology where the maximum yield of levan was found to be 268 μg/ml at a pH of 8.0, a temperature of 20°C and the incubation period of 24^th h. The pH- and thermal stabilities of levan-forming activity were studied and arrived at the maximum activity at pH 7.0 and at 20°C respectively, thereby inferring that the levan is optimally produced by Bacillus subtilis in an alkaline environment. Scanning electron microscope was used to examine the surface morphology levan-producing Bacillus subtilis that showed the levan polymer as white dust-like particles.

Keywords

Bacillus subtilis, Levan Polymer, RSM, Scanning Electron Microscope.

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D. Suresh ¹, P. Sridhar ¹, D. Joyce Hellen Sathya ², M. Anil Kumar ², M. Seenuvasan ², R. Dhandapani ³

Affiliations
1 Department of Biotechnology, Sengunthar Arts and Science College, Tiruchengode, IN
2 Department of Biotechnology, Madha Engineering College, Kunrathur, Chennai, IN
3 Department of Microbiology, Periyar University, Salem, IN

Abstract

A bacterium designated as Acinetobacter sp., isolated from domestic sewage soil identified by taxonomic studies by 16S rDNA sequential analysis was found to decolorize azo dye with the decolorization efficiency varying from 80-96%. This strain decolorized 96.2% a deep red sulphonated azo dye Reactive RedC2G29 (200 mg/L) with 1.76 g/L dry cells of specific decolorization rate in static condition at optimum pH 5.0 and temperature 30°C. The degradation efficiency of this strain was within 48 h. Phytotoxicity studies revealed less toxic nature of decolorized products as compared to original dye. HPLC, GC-MS and LC-MS analysis revealed the production of metabolites as final product by degradation of the dye.

Keywords

Reactive Red C2G29, Degradation, Acinetobacter sp.

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T. Uma ¹, V. N. Saranya ¹, M. Anil Kumar ¹, S. Selvanaveen ¹, R. Viveka ¹, M. Seenuvasan ¹

Affiliations
1 Department of Biotechnology, Madha Engineering College, Chennai, IN

Abstract

The extra cellular lipase produced by a novel strain of Bacillus sp. isolated from soil was used as an alternative for commercially available detergents for removing stains like oil, grease and blood stains in fabric clothes, as lipase shows the effect of hydrolysis of fat. The production was carried out by submerged fermentation using olive oil as substrate. The production media was optimized using the Response Surface Methodology (RSM) to get maximum yield with at most lipase activity. Thus Lipase produced had highest activity at pH 8, 30°C under incubation period of 24 h. The dialyzed sample of the partially purified enzyme was used to determine the molecular weight of lipase using sodium do-decyl sulphate poly acryl amide gel electrophoresis, which was 95 kDa. The stability of the enzyme was studied inferring it with maximum activity at pH 8.0 and temperature of 30°C. The detergent action of the enzyme was analyzed using the ultra filtered enzyme samples. The stain removal capacity of the lipase was better than the commercial detergents used.

Keywords

Bacillus sp., Lipase, Activity, pH, Temperature, RSM.

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M. Anil Kumar ¹, S. Keerthana ¹, Adyasa Pani ¹, D. Suresh ¹, M. Seenuvasan ¹

Affiliations
1 Department of Biotechnology, Madha Engineering College, Chennai, IN

Abstract

The combined effects of initial sucrose and initial Reactive Red C2G 29 dye concentrations on the specific growth rate and dye bioaccumulation efficiency of Penicillium chrysogenum MTCC 6477 was determined at pH 5.0. The highest growth rate of 0.115 h^-1 was obtained at an initial sucrose concentration of 15 g/L in absence of dye. For each constant sucrose concentration chosen between 5 and 15 g/L with an increase in initial dye concentration up to 500 mg/L under pH 5.0 resulted in a decrease in percentage uptake of dye and more over all dye concentration tested inhibited the growth of Penicillium chrysogenum. On the other hand at each constant dye concentration studied, both the specific growth rate and uptake yield enhanced with raising sucrose concentration up to15 g/L with pH 5.0, the maximum uptake yield of 96.67 % was observed in 15g/L sucrose and 50 mg/L dye containing growth medium at pH 5.0. To check the stability of this bioaccumulation process a continuous bioaccumulation system was developed and it shows monotonic damping oscillation where the dye uptake concentration approaches equilibrium.

Keywords

Penicillium chrysogenum, Mycelium, Bioaccumulation, Stability, Reactive Red.

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M. B. Nishita ¹, Remya Rachel Chacko ¹, M. Anil Kumar ¹, D. Suresh ¹, S. Selvanaveen ¹, M. Seenuvasan ¹

Affiliations
1 Department of Biotechnology, Madha Engineering College, Chennai 600 069, IN

Abstract

A novel fibrinolytic enzyme from two different blood fibrin Bos taurus and Ovis aries as an added ingredient by Rhizomucor sp. which was purified by ammonium sulphate precipitation, dialysis and ion exchange chromatography. The purification protocol resulted in 1.373 and 1.737 -fold purification of the enzyme. The apparent molecular weight of the enzyme was found to be 30.0kDa, determined by sodium dodecylsulphate poly acrylamide gel electrophoresis. Optimal temperature and pH of the nattokinase were to be at 37°C and 8.0 respectively. Upon the addition of zinc chloride the enzyme activity was increased while EDTA and PMSF inhibited the activity of the enzyme, indicating the presence of metalloproteases. The produced enzyme showcases the blood clot-busting activity.

Keywords

Nattokinase, Bos taurus, Ovis aries, Rhizomucor sp.

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Deena Durairaj ¹, M. Anil Kumar ¹, M. Seenuvasan ¹

Affiliations
1 Department of Biotechnology, Madha Engineering College, Chennai- 600 069, IN

Abstract

A sequential optimization based on statistiscal design was employed to optimize the media constituents for the improvement of citric acid production from cane molasses through surface fermentation using Aspergillus niger MTCC 282. The selected medium constituents were optimized by the central composite design under the response surface methodology where the maximum yield of citric acid and minimal residual sugar concentration was found to be 28.75 g/L and 0.9 g/L at pH at a temperature of 30ºC having a substrate concentration of 120 g/L under 9 days of incubation. The produced citric acid was then separated using cation-exchange chromatography.

Keywords

Citric Acid, Residual Sugar, Aspergillus niger, RSM.

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