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In vitro Antioxidant Activity of Bark and Leaf of Thespesia populnea


Affiliations
1 Himachal Institute of Pharmacy Education and Research Naudan, Bela, Hamirpur, Himachal Pradesh-177033, India
2 Himachal Institute of Pharmacy Education and Research, Naudan, Bela, Hamirpur, Himachal Pradesh-177033, India
     

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The free radical scavenging activity of Thespesia populnea bark (TPBE) and leaves (TPLE) has been studied using the methanolic extracts. In vitro antioxidant potential of leaves and stem bark were evaluated in three different models viz superoxide radical scavenging activity, lipid peroxide radical scavenging activity and hydroxyl radical scavenging activity. The two methanolic extract were prepared with different concentration from a maximum of 400μg to minimum of 50μg. All extract have different level of antioxidant property. In comparison, the antioxidant scavenging activity in hydroxyl radical was higher, In super oxide radical was moderate and lesser in lipid peroxide radical. These observations confirm that methanolic extract of Thespesia populnea bark and leaf have different polyphenolic constituent and its importance in antioxidant activity.

Keywords

Antioxidant, Thespesia populnea Leaf, Thespesia populnea Bark.
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  • In vitro Antioxidant Activity of Bark and Leaf of Thespesia populnea

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Authors

R. Parthasarathy
Himachal Institute of Pharmacy Education and Research Naudan, Bela, Hamirpur, Himachal Pradesh-177033, India
Amrender Singh
Himachal Institute of Pharmacy Education and Research Naudan, Bela, Hamirpur, Himachal Pradesh-177033, India
Debjit Bhowmik
Himachal Institute of Pharmacy Education and Research, Naudan, Bela, Hamirpur, Himachal Pradesh-177033, India

Abstract


The free radical scavenging activity of Thespesia populnea bark (TPBE) and leaves (TPLE) has been studied using the methanolic extracts. In vitro antioxidant potential of leaves and stem bark were evaluated in three different models viz superoxide radical scavenging activity, lipid peroxide radical scavenging activity and hydroxyl radical scavenging activity. The two methanolic extract were prepared with different concentration from a maximum of 400μg to minimum of 50μg. All extract have different level of antioxidant property. In comparison, the antioxidant scavenging activity in hydroxyl radical was higher, In super oxide radical was moderate and lesser in lipid peroxide radical. These observations confirm that methanolic extract of Thespesia populnea bark and leaf have different polyphenolic constituent and its importance in antioxidant activity.

Keywords


Antioxidant, Thespesia populnea Leaf, Thespesia populnea Bark.