Comparative Standardization of a Polyherbal Ayurvedic Formulation:Talisadi Churna

India is a land mark for the traditional system of medicine from the past few centuries. Most of the traditional system of medicine are effective but only one major drawback is lack of standardization. So, there is a need to develop standardization technique to mingle this system of medicine in the main stream of health sciences. Centre council for research in Ayurveda and siddha has given the preliminary guideline for standardizing these conventional formulations. The present paper reports standardization of Talisadi churna an Ayurvedic formulation, from three different companies. Three marketed samples were subjected to organoleptic study, physical characterization and chromatographic study. It was observed that all the formulations are similar with their organoleptic properties and to some extend to their physical characters but they do not match quantitatively in active constituent (piperine) content which may be due to variation in raw material like geographical variation, collection time, storage etc. This study ready reference for selection of an appropriate formulation in the clinical practice and hence effective rational therapy, the overall theme of health science. The physical data obtained from various samples shows different values as they are claimed to be of same plant material in same quantities. The qualitative estimation of volatile oil shows the presence in all the formulations. The HPLC results of quantitative estimation of piperin shows considerable variation in piperine content into the formulations in which the maximum concentration was obtained in the formulation A.