Research Journal of Pharmacognosy and Phytochemistry

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HPTLC Finger Print Analysis of Steroid, Flavonoid and Antiradical activity in Sargassum wightii from Gulf of Mannar


Affiliations
1 P.G. and Research Department of Plant Biology and Biotechnology, Botany Res, Laboratory, V. O. Chidambaram College, Tuticorin, 628 008, Tamil Nadu, India
2 P.G. and Research Department of Plant Biology and Biotechnology, Botany Res., Laboratory, V. O. Chidambaram College, Tuticorin, 628 008, Tamil Nadu, India
     

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The aim of this research was to estimate the steroid, flavonoid and antiradical activity of brown seaweed Sargassum wightii. The HPTLC analysis reveals the presence of polyphenolic signals at different ranges. The steroid and flavonoid content of ethanolic extract was observed in 0.35 Rf and presence of flavonoid in the given standard but not in the sample respectively. Scavenging activity was observed from 05 to 20mg/ml, 24 ± 1.3; 41.3 ± 2.4; 63.5 ± 2.3 and 73.1 ± 1.7, which showed significance of the active principles in S. wightii.


Keywords

Sargassum wightii, Steroid, Flavonoid, Antiradical Activity, HPTLC.
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  • HPTLC Finger Print Analysis of Steroid, Flavonoid and Antiradical activity in Sargassum wightii from Gulf of Mannar

Abstract Views: 231  |  PDF Views: 0

Authors

V. Madhan Chakkaravarthy
P.G. and Research Department of Plant Biology and Biotechnology, Botany Res, Laboratory, V. O. Chidambaram College, Tuticorin, 628 008, Tamil Nadu, India
Venkataraman Kumar
P.G. and Research Department of Plant Biology and Biotechnology, Botany Res., Laboratory, V. O. Chidambaram College, Tuticorin, 628 008, Tamil Nadu, India

Abstract


The aim of this research was to estimate the steroid, flavonoid and antiradical activity of brown seaweed Sargassum wightii. The HPTLC analysis reveals the presence of polyphenolic signals at different ranges. The steroid and flavonoid content of ethanolic extract was observed in 0.35 Rf and presence of flavonoid in the given standard but not in the sample respectively. Scavenging activity was observed from 05 to 20mg/ml, 24 ± 1.3; 41.3 ± 2.4; 63.5 ± 2.3 and 73.1 ± 1.7, which showed significance of the active principles in S. wightii.


Keywords


Sargassum wightii, Steroid, Flavonoid, Antiradical Activity, HPTLC.