Evaluation of Antimicrobial Activity of Tephrosia procumbens Buch–Ham

In the present study, the entire plant of Tephrosia procumbens Buch-Ham commonly known as Vempalli, Indigo sauvage (Fam-Fabaceae/ Leguminosae) was investigated for the phytochemical screening and antimicrobial property against certain microorganisms using disc diffusion method. The entire plant of Tephrosia procumbens were collected, shade dried at room temperature, pulverized and extracted with 95% ethanol in soxhlet extractor to get total ethanolic extract which is further fractionated with the solvents of different polarities. Total aqueous extract was also obtained by macerating the shade dried material with 3% of chloroform water I.P. All the extracts and fractions were subjected for Preliminary Phytochemical screening, which has shown the presence of Carbohydrates, Steroids and Flavonoids and evaluation of antimicrobial property by using disc diffusion method on various pathogenic forms of microorganisms including gram positive, gram negative and fungi. It was found that total ethanolic and total aqueous extract of entire plant of Tephrosia procumbens significantly inhibit the growth of microorganisms as compared to standard drugs Gentamycin and streptomycin (Hi-Media Lab. Mumbai).