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A Pharmacognostical Study on Albizzia lebbeck Bark


Affiliations
1 Department of Pharmacognosy, APMC College of Pharmaceutical Education Research, Himatnagar, India
2 Dept. of Pharmacognosy, L. M. College of Pharmacy, Ahmedabad, India
3 Dept. of Pharmacognosy, A. R. College Pharmacy, Vallabh Vidyanagar, India
     

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Fresh stembark of Albizzia lebbeck was studied for macro and microscopical characters. Betulinic acid was isolated and identified by M. P., co-chromatography with reference standard and IR spectral characteristics. HPTLC method was developed for quantification of betulinic acid using precoated silica gel plates as a stationary phase, Toluene: Acetone: Acetic acid (100:1:0.1) as a mobile phase and anisaldehyde sulphuric acid as a spray reagent. The bark is rough as longitudinal fissures and transverse cracks are present. TS of bark shows rhytidoma as a major portion followed by stone cell bands and secondary phloem with groups of phloem fibres, with starch and prisms of calcium oxalate being found in all the tissues. Saponins, tannins, triterpenoids and flavanoids were found be the major components of the bark. HPTLC method was developed for quantification of betulinic acid by scanning the plates at 523nm. The quality parameters and HPTLC method developed would serve as useful tools in standardization of Albizzia lebbeck.

Keywords

Albizzia lebbeck, HPTLC, Quality Parameters, Betulinic Acid.
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  • A Pharmacognostical Study on Albizzia lebbeck Bark

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Authors

U. D. Shah
Department of Pharmacognosy, APMC College of Pharmaceutical Education Research, Himatnagar, India
M. B. Shah
Dept. of Pharmacognosy, L. M. College of Pharmacy, Ahmedabad, India
A. K. Saluja
Dept. of Pharmacognosy, A. R. College Pharmacy, Vallabh Vidyanagar, India

Abstract


Fresh stembark of Albizzia lebbeck was studied for macro and microscopical characters. Betulinic acid was isolated and identified by M. P., co-chromatography with reference standard and IR spectral characteristics. HPTLC method was developed for quantification of betulinic acid using precoated silica gel plates as a stationary phase, Toluene: Acetone: Acetic acid (100:1:0.1) as a mobile phase and anisaldehyde sulphuric acid as a spray reagent. The bark is rough as longitudinal fissures and transverse cracks are present. TS of bark shows rhytidoma as a major portion followed by stone cell bands and secondary phloem with groups of phloem fibres, with starch and prisms of calcium oxalate being found in all the tissues. Saponins, tannins, triterpenoids and flavanoids were found be the major components of the bark. HPTLC method was developed for quantification of betulinic acid by scanning the plates at 523nm. The quality parameters and HPTLC method developed would serve as useful tools in standardization of Albizzia lebbeck.

Keywords


Albizzia lebbeck, HPTLC, Quality Parameters, Betulinic Acid.