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LCMS Spectral Analysis of Betacyanin Pigments in Amaranthus L.


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1 Depatment of Botany, St.Teresa’s College, Ernakulam, Kerala 682011, India
     

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Plant pigments has got wider application in textile and pharmaceutical industry. Betacyanins are classes of purple plant pigments that are useful in plant identification and health monitoring. Pure samples of betacyanin were obtained by extracting and isolating the pigments found in Amaranthus L. plants. The resulting samples were then used to identify the absorption spectra of the pigment. The sample were analysed for measuring the absorbance at the range of 476nm and 538nm respectively. Betanin, Neobetanin and Amaranthine were found in the red pigments isolated from the plants which are of pharmaceutically important and got medicinal application. These pigments play important role as antioxidant and a natural dye for different formulations. The findings from the present spectral analysis showed 382 m/z ratio found to be derivative of betanidin component. LCMS analysis of the red pigment were carried out showing positive peak with 389 m/z ratio for betanidin compound among Amaranthus caudatus L. and Amaranthus dubius, Mart along with other peak values. The antioxidant capacity that is to scavenge the free radicals of these two Amaranthus L. species can be taken into account as it is a good dietary supplement in our daily life.

Keywords

Betacyanin, Betanin, Neobetalin , Amaranthine, LCMS.
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  • LCMS Spectral Analysis of Betacyanin Pigments in Amaranthus L.

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Authors

Pinkie Cherian
Depatment of Botany, St.Teresa’s College, Ernakulam, Kerala 682011, India
D. Sheela
Depatment of Botany, St.Teresa’s College, Ernakulam, Kerala 682011, India

Abstract


Plant pigments has got wider application in textile and pharmaceutical industry. Betacyanins are classes of purple plant pigments that are useful in plant identification and health monitoring. Pure samples of betacyanin were obtained by extracting and isolating the pigments found in Amaranthus L. plants. The resulting samples were then used to identify the absorption spectra of the pigment. The sample were analysed for measuring the absorbance at the range of 476nm and 538nm respectively. Betanin, Neobetanin and Amaranthine were found in the red pigments isolated from the plants which are of pharmaceutically important and got medicinal application. These pigments play important role as antioxidant and a natural dye for different formulations. The findings from the present spectral analysis showed 382 m/z ratio found to be derivative of betanidin component. LCMS analysis of the red pigment were carried out showing positive peak with 389 m/z ratio for betanidin compound among Amaranthus caudatus L. and Amaranthus dubius, Mart along with other peak values. The antioxidant capacity that is to scavenge the free radicals of these two Amaranthus L. species can be taken into account as it is a good dietary supplement in our daily life.

Keywords


Betacyanin, Betanin, Neobetalin , Amaranthine, LCMS.

References