Research Journal of Pharmacology and Pharmacodynamics

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Evaluation of Gastroprotective Influence of Vitamin C, Melatonin, Rabeprazole and their Combination in Ethanol Induced Ulcer Model


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1 HKES College of Pharmacy, Sedam Road, Gulbarga–585 105 Karnataka, India
     

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Aim: To evaluate the gastroprotective effect of the antioxidants (vitamin C and melatonin), rabeprazole and their combination in ethanol induced peptic ulcers.
Methodology: The study was divided into 2 dosage groups-single and multiple dose study groups. Each of the dosage group consists of wistar albino rats of either sex (180-220g) divided into 4 groups of 6 animals each.
In single dose study, The animals were fasted for 48h. At the end of 48h, the animals were administered with the drugs. 1h after drug administration, absolute ethanol (1ml/200g p.o.) was administered to all groups. 2h after ethanol administration, the animals were euthantised and stomachs were excised out. In multiple dose study, the animals were administered with drugs for 7 consecutive days. On 7th day following drug administration, the animals were fasted for 48h. At the end of 48h, the aimals were administered with absolute ethanol (1ml/200g p.o.) 2h after ethanol administration, the animals were euthantised, stomachs were excised out. The stomachs excised out from both the study groups were evaluated for ulcer index and percentage protection. However, for multiple dose study group histopathological study was also performed to evaluate cytoprotection.
Result: The results were interpreted statistically using one way ANOVA. The combination group (rabeprazole + vitamin C and rabeprazole + Melatonin) showed significant antiulcer activity in both single and multiple dose study groups, on comparison with rabeprazole, vitamin C and melatonin individually.
Conclusion: The effect of combination groups was found to be synergistic in nature and more effective in treating peptic ulcer disease.

Keywords

Antiulcer activity, Rabeprazole, Vitamin C, Melatonin, Antioxidants, Ethanol induced ulcers
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  • Goodman and Gilman. The pharmacological basis of therapeutics. 10th Edition. New York, McGraw Hill: 2001: 1007.

  • http://www.wikipedia.org/wiki/pepticulcers.htm

  • Ronald Fernandes. Ph.D. Dissertation work submitted to the Mangalore University. Gastroprotective and antisecretory effect of selenomethionine in albino rats 2008: 1.

  • http://www.umm.edu/digest/ulcers.htm.

  • Parmar NS, Jagruti K Desai. A review of the current methodology for the evaluation of gastric & duodenal anti-ulcer agents. Indian J Pharmacol 1993; 25: 120-35.

  • Morris GP and Wallece JL: The role of ethanol inf acid production of gastric mucosal erosions in rats. Virchows Arch 46:1981; 239-51.

  • Qui BS, Cho CH and Ogle CW: Chronic nicotine treatment intensifies gastric ulceration by cold restraint stress in rats. Agents Actions 33: 1991; 367-70.

  • Smura H and Marumo F: Effect of acetaminophen on stress induced gastric mucosal lesion in rats. Res Comm. Mol Path Pharmacol 86(3):1994; 297-310.

  • Sharma HL and Sharma KK. Principles of Pharmacology. 1st Edition, Paras publications, 2007: 368.

  • Bandyopadhyay D, Chattopadhyay A. Reactive oxygen speciesinduced gastric ulceration, protection by melatonin. Curr. Med chem. 2006; 13(10): 1187-1202.

  • Manjeet singh, Neelam jaiswal. Oxygen derived free radicals and circulatory shock. Ind J Pharmacol. 1992; 24: 8-11

  • Venkatesham A, Sharath babu P, Vidyasagar J, Krishna DR. Effect of reactive oxygen species on cholinergic receptor function. Ind J Pharmacol. 2005; 37(6): 366-70.

  • Langseth, L. 1993. Oxidants, antioxidants and disease prevention. International Life Sciences Institute, 1-26.

  • Dr Dhrubo Jyoti Sen. Herbal Antioxidants: A Great Hope for future. Pharma Times. 2008; 40(12): 22-36.

  • http://www.merckmanuals.com

  • 48. Reiter RJ, Tan DX, Sainz RM, Mayo JC, Lopez Burillo S. Melatonin reducing the toxicity and increasing the efficacy of drugs. Journal of Pharmacy and Pharmacology. 2002; 54(10): 1299-1321.

  • www.janssen-ortho.com

  • Goodman and Gilman. The pharmacological basis of therapeutics. 10th Edition. New York, McGraw Hill: 2001: 1007.

  • Avila JR, Lastra CA, Martin MJ. Motilava V, Lauque I, Delgado D, Esteban J and Herrevcas J; role of endogenous sulphahydryl and neutrophil infiltration in pathogenesis of gastric mucosal injury induced by piroxicam in rats. Inflam Res. 45:1996; 83-8.

  • Ronald Fernandes. Ph.D. Dissertation work submitted to the Mangalore University. Gastroprotective and antisecretory effect of selenomethionine in albino rats 2008: 45.

  • Kulkarni SK. Hand book of experimental pharmacology 3rd edition, Vallabh Prakashan, New Delhi. 2005:148-50.

  • Fathihah B, Mahmood AA, Sidik K and Salmah I. The antiulcer and cytoprotective effect of ageratum conyzoides–honey combination in rats, Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, JUMMEC 2003-2005; 8: 28-32.

  • Deshpande SS, Shah GB, Parmar NS. Antiulcer activity of Tephrosia purpurea in rat. Ind.J of pharmacology. 2003; Vol 35: 168-72.


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  • Evaluation of Gastroprotective Influence of Vitamin C, Melatonin, Rabeprazole and their Combination in Ethanol Induced Ulcer Model

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Authors

Nitin Mahurkar
HKES College of Pharmacy, Sedam Road, Gulbarga–585 105 Karnataka, India
S. M. Sayeed Ul hasan
HKES College of Pharmacy, Sedam Road, Gulbarga–585 105 Karnataka, India
A. Arati Malpani
HKES College of Pharmacy, Sedam Road, Gulbarga–585 105 Karnataka, India
Chetan Manjunath
HKES College of Pharmacy, Sedam Road, Gulbarga–585 105 Karnataka, India

Abstract


Aim: To evaluate the gastroprotective effect of the antioxidants (vitamin C and melatonin), rabeprazole and their combination in ethanol induced peptic ulcers.
Methodology: The study was divided into 2 dosage groups-single and multiple dose study groups. Each of the dosage group consists of wistar albino rats of either sex (180-220g) divided into 4 groups of 6 animals each.
In single dose study, The animals were fasted for 48h. At the end of 48h, the animals were administered with the drugs. 1h after drug administration, absolute ethanol (1ml/200g p.o.) was administered to all groups. 2h after ethanol administration, the animals were euthantised and stomachs were excised out. In multiple dose study, the animals were administered with drugs for 7 consecutive days. On 7th day following drug administration, the animals were fasted for 48h. At the end of 48h, the aimals were administered with absolute ethanol (1ml/200g p.o.) 2h after ethanol administration, the animals were euthantised, stomachs were excised out. The stomachs excised out from both the study groups were evaluated for ulcer index and percentage protection. However, for multiple dose study group histopathological study was also performed to evaluate cytoprotection.
Result: The results were interpreted statistically using one way ANOVA. The combination group (rabeprazole + vitamin C and rabeprazole + Melatonin) showed significant antiulcer activity in both single and multiple dose study groups, on comparison with rabeprazole, vitamin C and melatonin individually.
Conclusion: The effect of combination groups was found to be synergistic in nature and more effective in treating peptic ulcer disease.

Keywords


Antiulcer activity, Rabeprazole, Vitamin C, Melatonin, Antioxidants, Ethanol induced ulcers

References