Research Journal of Pharmacology and Pharmacodynamics

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Cardioprotective Activity of Ocimum canum Hydro-Alcoholic Leaf Extracts against Isoproterenol Induced Myocardial Infarction in Rats


Affiliations
1 Department of Pharmacology, Royal College of Pharmacy and Health Sciences, Brahmapur, Odisha- 760002, India
2 Department of Pharmacology, SIMS College of Pharmacy, Mangaldas Nagar, Guntur- 522001, India
3 Department of Pharmacology, MKCG Medical College, Berhampur, Odisha- 760004, India
     

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Myocardial infarction (MI) was produced in rats with 200 mg/kg of isoproterenol (ISO) administered subcutaneously (sc) twice at an interval of 24 h. Shift in antioxidant parameters, lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine phosphokinase (CPK), Troponin T, Catalase together with morphological and histopathological changes were investigated. Two hundred mg/kg ISO dose was selected for the present study as this dose offered significant alteration in biochemical parameters along with moderate necrosis in heart. Effect of pre- treatment of hydro-alcoholic extract of Ocimum canum (OC) at different doses (100, 200 and 400 mg/kg) was investigated against ISO (200 mg/kg) induced myocardial infarction in rats. Modulation of various biochemical parameters and membrane integrity was studied. OC at the dose of 200 and 400 mg/kg reduced significantly glutathione (GSH), superoxide dismutase (SOD) and LDH levels. It also inhibited the lipid peroxidation as observed by the reduced thiobarbituric acid reactive substances (TBARS) levels. In the present study OC at the dose of 400 mg/kg was found to demonstrate maximum cardio-protective effect. Above results were further confirmed by histopathological findings. Thus from the present study it is concluded that OC may be of therapeutic and prophylactic value in the treatment of MI.

Keywords

Isoproterenol, Myocardial Infarction, Ocimum canum, Antioxidant Enzymes.
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  • Cardioprotective Activity of Ocimum canum Hydro-Alcoholic Leaf Extracts against Isoproterenol Induced Myocardial Infarction in Rats

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Authors

Saiprasanna Behera
Department of Pharmacology, Royal College of Pharmacy and Health Sciences, Brahmapur, Odisha- 760002, India
S. Manohar Babu
Department of Pharmacology, SIMS College of Pharmacy, Mangaldas Nagar, Guntur- 522001, India
Y. Roja Ramani
Department of Pharmacology, MKCG Medical College, Berhampur, Odisha- 760004, India
Prasanta Kumar Choudhury
Department of Pharmacology, Royal College of Pharmacy and Health Sciences, Brahmapur, Odisha- 760002, India
Rajeshree Panigrahi
Department of Pharmacology, Royal College of Pharmacy and Health Sciences, Brahmapur, Odisha- 760002, India

Abstract


Myocardial infarction (MI) was produced in rats with 200 mg/kg of isoproterenol (ISO) administered subcutaneously (sc) twice at an interval of 24 h. Shift in antioxidant parameters, lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine phosphokinase (CPK), Troponin T, Catalase together with morphological and histopathological changes were investigated. Two hundred mg/kg ISO dose was selected for the present study as this dose offered significant alteration in biochemical parameters along with moderate necrosis in heart. Effect of pre- treatment of hydro-alcoholic extract of Ocimum canum (OC) at different doses (100, 200 and 400 mg/kg) was investigated against ISO (200 mg/kg) induced myocardial infarction in rats. Modulation of various biochemical parameters and membrane integrity was studied. OC at the dose of 200 and 400 mg/kg reduced significantly glutathione (GSH), superoxide dismutase (SOD) and LDH levels. It also inhibited the lipid peroxidation as observed by the reduced thiobarbituric acid reactive substances (TBARS) levels. In the present study OC at the dose of 400 mg/kg was found to demonstrate maximum cardio-protective effect. Above results were further confirmed by histopathological findings. Thus from the present study it is concluded that OC may be of therapeutic and prophylactic value in the treatment of MI.

Keywords


Isoproterenol, Myocardial Infarction, Ocimum canum, Antioxidant Enzymes.

References