Research Journal of Pharmacology and Pharmacodynamics

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Evaluation of Effect of Murraya koenigii on Restraint Stress Induced Perturbations


Affiliations
1 Bombay College of Pharmacy Santacruz (E) Mumbai, Maharashtra, India
2 C.U. Shah College of Pharmacy, SNDT University Santacruz (W), Mumbai 400049, Maharashtra, India
     

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Aqueous (MKAQ), Hydroalcoholic (MKHA) and Methanolic extract (MKM) of leaves of Murraya koenigii (MK) at dose levels 50mg/kg 100mg/kg and 200mg/kg p.o. were investigated to gauge antistress activity in fresh wistar rats. Anti anxiety drug Diazepam (1mg/kg p.o.) and natural antistress Ashwagandha (AS) (100mg/kg p.o.) were used as standards. The animals were subjected to restraint stress (2 hrs/day) for 14 days to evaluate the anti-stress potential in chronic stress condition. Further they were tested to observe their effects on retrograde amnesia caused by chronic stress in elevated plus maze and Step down inhibitory avoidance tests. Nootropic drug Piracetam (200mg/kg p.o.) was used as a standard for cognition studies. Stimulation of hypothalamus pituitary adrenal axis in stressful condition alters corticosterone, glucose, triglyceride and cholesterol, levels. There is also alteration in the norepinephrine 5HT and dopamine levels in the brain. Treatment with the extracts significantly ameliorated the stress-induced variations in these biochemical and bioamine levels. Treatment with MKM, MKAQ and MKHA extracts also prevented stress induced adrenomegaly . In cognition studies stress induced increase in latency period and decrease in step down latency in elevated plus maze models and Step down inhibitory avoidance test respectively was prevented. The results indicate that among three test extracts MKM was the most effective extract as compared to MKHA and MKAQ extracts of the plant thus possessing significant antistress potential against a variety of biochemical and physiological perturbations during stress.

Keywords

Chronic Restraint Stress, Murraya koenigii, Corticosteron, Bioamines, Antistress.
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  • Selye H. The evolution of the stress concept. Am. Sci. 1973, 61, 693-699

  • Mason J. W. A review of psychoendocrine research on the sympatho-adrenal medullary system. Psychosom. Med.1968, 30: 631-683

  • Selye H. The physiology and pathophysiology of exposure to stress. Montreal: Acta; 1950.

  • Elizabeth O. Johnson,T. Themis c. kamilaris,T George, P. Chrousos and Philip W. Gold, Mechanisms of Stress: A Dynamic Overviewof Hormonal and Behavioral Homeostasis Neuroscience and Biobehavioral Reviews, 1992 Vol. 16, 115-130

  • Lupien S J, Lepage M. Stress, memory, and the hippocampus: can't live with it, can't live without it. Behav Brain Res 2001;127:137-58.

  • Lupien S J, McEwen BS. The acute effects of corticosteroids on cognition: integration of animal and human model studies. Brain Res Rev 1997;24:1-27.

  • Kloet R L. Stress in the brain. Eur J Pharmacol 2000;405:187-98.

  • H olscher C. Stress impairs performance in spatial water maze learning tasks. Behav Brain Res 1999;100: 225-30

  • Lupien S J, Lepage M. Stress, memory, and the hippocampus: can't live with it, can't live without it. Behav Brain Res 2001;127: 137-58.

  • Mabry TR, Gold PE, Mc Carty R. Stress, aging and memory. Involvement of peripheral catecholamines. Ann NY Acad Sci 1995;771: 512-22.

  • Nishimura J, Endo Y, Kimura F. A long-term stress impairs maze learning performance in rats. Neurosci Lett 1999;273:125-8.

  • Thomas, Samuel, Mathew, Baby, P. Sakaria ,Medicinal plants, Kerala Agricultural Unmiversity, Aromatic and Medicinal plant research center, 1998, p87-88, 13.

  • Kirtikar, Basu, Indian Medicinal plants 1975,Vol-I, 472-474

  • E. Edwin Jarald, E.Sheeja, S.Parial, H.Arya, and A. Bajpai, Morphoanatomy of stems of Murraya Koenigii, Journal of biological science 2008, p.1-5

  • Math MV and Balasubramaniam P. Curry leaves(Murraya Koenigii spreng) and halitosis. BMJ SouthAsia edition 2003;19(3):211

  • M.M, Ali A M, Elshakawy, S.I., F. Manup, Antimicrobial and cytotoxic properties of some malayasion traditional vegetables Int J pharmacology 1997, 35 p 1-5

  • KureelS.P., Kapil R.S. and Popli S.P New alkaloids from Murrya Koenigii. spreg, Experientia. 1969 b, 25, 790

  • Narsinhan N.S., Paradkar M.V., Kelkar S.L, Alkaloids of Murraya Koenigii Structures of mahanine, koenine, koenigine and koenidine. Indian J chem1970B, vol . 8, 473

  • Joshi, B.S Kamat, V.N. and .Gawad, D.H On the structures of Girinimbine, Mahanimbine, Isomahanimbine ,Koenimbidine and Murrayacine 1970 ,Tetrahedron26, 1475-1483.

  • Kureel S.P.,Kapil R.S. and Popli, S.P. Terpenoid alkaloids from Murraya Koenigii SprengIV. Structure and synthesis of mahanimbinine Experentia,1970b ,26,1055

  • Kureel S.P., Kapil R.S. and Popli, S.P Two Novel alkaloids from Murraya Koenigii spreng Mahanimbicine and bicyclomahanimbicine Chem Ind 1970c No29,958.

  • Narsimhan N.S. , Paradkar, M.V., Chitguppi , V.P. and Kelkar S.L. Alkaloids of Murraya Koenigii Structures of Mahanimbine,Koenimbine,mahanine,koenine,koenigine,koenidine,isomahanimbine Indian J Chem. 1975 13: 993

  • Anwar F, Kapil R.S., Popli S.P., Terpenoid alkaloids fron Murraya Konigii spreg.VII synthesis of DL-O-methylmahanine and related carbazoles. Experientia 1972 ,28,769

  • Gupta G.L and NigamS.S., 1970, chemical examination of the leaves of Murraya koenigii planta med. 19, 83

  • Gupta G L and Nigam, S. S, Chemical examination of the leaves of Murraya koenigii coumarine glycoside ,1970,Planta Med 19,83

  • Chowdhary J.J, Bhuiyan N. I., Yusuf chemical composition of the leaf essential oils of Murraya Koenigii(L.) spreng and Murraya paniculata(L.)Babgladesh J Pharmacol 2008,3, 59-63

  • Neuroscience and Biobehavioral Reviews, Restraint Stress in Biomedical Research: A Review I 1986, Vol. 10, 339-370

  • Ciarlone A. E. Further modification of a Fluorometric Method for Analyzing Brain Amines Microchemical Journal 1978, 23:9-12

  • Sharma A.Kulkarni S.K.Evaluation of learning and memory mechanism employing plus maze in rats and mice" Prog Neuropsychopharmacol Biol Psychiatry,16 1191,125.

  • Jaiswal AK, Bhattacharya SK. Effect of prenatal under nutrition and phenobarbitone administration on discrimination learning and passive avoidance behaviour in rats. Indian J Exp Biol 1996;34:118- 23.

  • Gutierre-Figueroa GP, Dalmaz CandIzquierdo,Effects of entorhinal cortex lesions on memory in different tasks.Brazillian Journal of medical and biological research,1993,30:769-774.

  • Vernikos, J., Dallman, M.F., Bonner, C., Katzen, A., Shinsako, J., 1982. Pituitary adrenal function in rats chronically exposed to cold. Endocrinology, 1982 110, 413-420, 2.Vernikos et al.,

  • Rai D., Bhatia, G., Sen T., Palit G., 2003a. Comparative study of perturbations of peripheral markers in different stressors in rats. Canadian Journal of Physiology and Pharmacology, 2003a 81, 1139-1146.

  • Dadkar V.N.,Ranadire N.J.,Dhar H.L. Evaluation of antistress (Adaptogenic ) activity of Withania somnifera.Indian Jclin.Biochem 1987,2:101-108

  • Chrousos G P, Gold P W. The concept of stress and stress system disorders.JAMA 1992;267: 1244-52.

  • Achyut Narayan Kesari, Rajesh Kumar Gupta, Geeta Watal Hypoglycemic effects of Murraya koenigii on normal andalloxan-diabetic rabbits Journal of Ethnopharmacology 2005 97:247-251

  • Arulselvon G.P. Senthikumar D satish kumar and subramanian 6 Antidiabetic effect of Murraya Koenigii leaves on streptozocine induced diabetic rats, Pharmagazine, 2006. 61(10) : 874-877.

  • Bijlani H.L.,Sud S,Gandhi B.M.,Tandon B.N Relationship of examination stress to serum lipid profile, Indian Journal of Physiology and pharmacology 1985,30(1):22-30

  • Glavin G.B. Selective noradrenaline depletion markedly alters stress responses in rats Life Sciences, 198 Vol. 37,No-5 pp. 461-465,

  • Singh N, Mishra N , Srivastav A K, Dixit K S, Gupta G P Effect of Antistress plants on biochemical changes during stress reaction. Indian J of pharmacology,1991, 23:137-142

  • Inoue T T ,suchiya K, Koyama T Regional changes in dopamine and serotonin activation with various intensity of physical and psychological stress in rat brain Pharmacol Biochem Behav. 1994,49:911-920

  • Bhattacharya SK. Evaluation of adaptogenic activity of Trasina, an Ayurvedic herbal formulation. In: Mukherjee B, editor. Traditional medicine.New Delhi: Oxford and IBH Publishing; 1993. 320-6.

  • Vasudevan M.,Parle M.,Sengottuvelu S,Shanmugapriya T.Nootropic potential of Murraya Koenigii leaves in rats.,Oriental pharmacy and Experimental Medicine,2008 8(4),365-373.

  • Singh, V.B., Onavi, E.S., Phan, T.H., Boadle-Biber, M.C., The increase in rat cortical and midbrain tryptophan hydroxylase activity in response to acute or repeated sound stress are blocked by bilateral lesions to the central nucleus of the amygdala. Brain Research 1990., 530, 49-53


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  • Evaluation of Effect of Murraya koenigii on Restraint Stress Induced Perturbations

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Authors

M. N. Saraf
Bombay College of Pharmacy Santacruz (E) Mumbai, Maharashtra, India
M. M. Sanaye
C.U. Shah College of Pharmacy, SNDT University Santacruz (W), Mumbai 400049, Maharashtra, India
S. A. Mengi
C.U. Shah College of Pharmacy, SNDT University Santacruz (W), Mumbai 400049, Maharashtra, India

Abstract


Aqueous (MKAQ), Hydroalcoholic (MKHA) and Methanolic extract (MKM) of leaves of Murraya koenigii (MK) at dose levels 50mg/kg 100mg/kg and 200mg/kg p.o. were investigated to gauge antistress activity in fresh wistar rats. Anti anxiety drug Diazepam (1mg/kg p.o.) and natural antistress Ashwagandha (AS) (100mg/kg p.o.) were used as standards. The animals were subjected to restraint stress (2 hrs/day) for 14 days to evaluate the anti-stress potential in chronic stress condition. Further they were tested to observe their effects on retrograde amnesia caused by chronic stress in elevated plus maze and Step down inhibitory avoidance tests. Nootropic drug Piracetam (200mg/kg p.o.) was used as a standard for cognition studies. Stimulation of hypothalamus pituitary adrenal axis in stressful condition alters corticosterone, glucose, triglyceride and cholesterol, levels. There is also alteration in the norepinephrine 5HT and dopamine levels in the brain. Treatment with the extracts significantly ameliorated the stress-induced variations in these biochemical and bioamine levels. Treatment with MKM, MKAQ and MKHA extracts also prevented stress induced adrenomegaly . In cognition studies stress induced increase in latency period and decrease in step down latency in elevated plus maze models and Step down inhibitory avoidance test respectively was prevented. The results indicate that among three test extracts MKM was the most effective extract as compared to MKHA and MKAQ extracts of the plant thus possessing significant antistress potential against a variety of biochemical and physiological perturbations during stress.

Keywords


Chronic Restraint Stress, Murraya koenigii, Corticosteron, Bioamines, Antistress.

References