Analgesic, Antipyretic, CNS and PASS Prediction Activities of 4-(4-Hydroxy Benzylidene Amino) Phenol Schiff Base

The Schiff base 4-(4-hydroxy benzylidene amino)phenol was prepared from p-hydroxybenzaldehyde and 4-aminophenol by condensation method using standard procedure. Analgesic, antipyretic and CNS activities of 4-(4- hydroxy benzylidene amino) phenol Schiff base were studied using albino rats of both the sexes. Animals were divided into three groups, each consisting of four animals. Group 1 served as control and group 2 received standard drug. Group 3 received 250 mg/kg of 4-(4-hydroxy benzylidene amino) phenol. For the determination of antipyretic activity, pyrexia was induced by 20% yeast suspension. The analgesic activity was determined by tail immersion method. CNS depressant activity of the compound was measured by placing the rat individually in the actophotometer for 10 min. The results obtained showed that the Schiff base was found to exhibit analgesic, antipyretic and CNS activities. The analgesic activity of the Schiff base compared to the standard drug pentazocine was found to be higher in the first hour and then the activity decreases slowly. The reduction in the rectal temperature for this compound was observed to be less than the standard drug paracetamol. 4-(4-hydroxy benzylidene amino)phenol Schiff base of 250mg/kg possessed CNS stimulant activity with a probability <0.001. 4-(4-hydroxy benzylidene amino)phenol Schiff base was found to possess highest glutathione thiolesterase inhibitor activity as 86% and other activities greater than 80% as per the prediction using PASS (online bioactivity prediction software) .