South Indian Journal of Biological Sciences

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A New Method of Approaching the Heart for Rapid Bleeding in Fish


Affiliations
1 Department of Zoology and Biotechnology, A.V.V.M. Sri Pushpam College (Autonomous), Thanjavur, Tamil Nadu, India
2 Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration, Cheonan, Korea, Republic of
3 Centre for Research and Development, PRIST University, Thanjavur, Tamil Nadu, India
4 Department of Zoology, Rajah Serfoji Government College (Autonomous), Thanjavur, Tamil Nadu, India
 

Bleeding is an extending art and basic for immunology. Expel of the blood from the fish is more complicated which may lead to stress or death to the fish. This new method of direct heart puncture, we can draw almost 650 - 750 μl of blood from 75.55 ± 4.75 gm weight of fish Cyprinus carpio using 1 ml tuberculin syringe. This method of bleeding can be completed within 28-35 sec, so that it can drastically reduce the stress to the fish. This bleeding method is effectively adapted in other fish species such as Catla catla and Oreochromis mossambicus.

Keywords

Cyprinus carpio, Catla, Bleeding, Direct Heart Puncture, Arch, Immune.
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  • A New Method of Approaching the Heart for Rapid Bleeding in Fish

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Authors

B. Kaleeswaran
Department of Zoology and Biotechnology, A.V.V.M. Sri Pushpam College (Autonomous), Thanjavur, Tamil Nadu, India
S. Ilavenil
Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration, Cheonan, Korea, Republic of
D. Karthik
Centre for Research and Development, PRIST University, Thanjavur, Tamil Nadu, India
S. Ravikumar
Department of Zoology, Rajah Serfoji Government College (Autonomous), Thanjavur, Tamil Nadu, India

Abstract


Bleeding is an extending art and basic for immunology. Expel of the blood from the fish is more complicated which may lead to stress or death to the fish. This new method of direct heart puncture, we can draw almost 650 - 750 μl of blood from 75.55 ± 4.75 gm weight of fish Cyprinus carpio using 1 ml tuberculin syringe. This method of bleeding can be completed within 28-35 sec, so that it can drastically reduce the stress to the fish. This bleeding method is effectively adapted in other fish species such as Catla catla and Oreochromis mossambicus.

Keywords


Cyprinus carpio, Catla, Bleeding, Direct Heart Puncture, Arch, Immune.

References