South Indian Journal of Biological Sciences

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Isolation and Characterization of Mannose-Binding Lectin Gene from Leaves of Allium ascalonicum (Shallot) and its Putative Role in Insect Resistance


Affiliations
1 Department of Plant Morphology and Algology, Madurai Kamaraj University, Madurai, Tamil Nadu–620 021, India
2 Department of Biotechnology, Mepco Schlenk Engineering College, Sivakasi, Tamil Nadu‐ 626 005, India
 

Plant lectins are the heterogenous group of glycoproteins extensively studied for their potent insecticidal property against Hemipteran pests. In this present study, the full‐length cDNA of monocot mannose‐binding insecticidal lectin gene was isolated from Allium ascalonicum leaves. The isolated Allium ascalonicum Lectin (AAL) gene was cloned in pGEM‐T vector, sequenced and the sequence was submitted to GenBank (KM096570.1). Sequence analysis revealed a 468 bp open reading frame (ORF) encoding a putative 155 amino acids agglutinin precursor. Multiple sequence alignment and phylogenetic analysis of AAL amino acid with those of 30 other Mannose binding lectin (MBL) sequences in NCBI revealed a high similarity of 85‐95% indicating that AAL is a member of the MBL super family and forms a cluster with other onion lectins. Secondary structure prediction and the homology modeling showed that AAL protein possess predominantly β‐sheets and three potential mannose‐binding motifs consisting of 5 amino acid residues QDNVY like other GNA lectins. The results of the insilico analysis predict that the Allium ascalonicum lectin gene can be another potent insecticidal protein.

Keywords

Hemipterans, Allium ascalonicum, Mannose Binding Lectins, In-Silico Analysis.
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  • Isolation and Characterization of Mannose-Binding Lectin Gene from Leaves of Allium ascalonicum (Shallot) and its Putative Role in Insect Resistance

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Authors

Ilamathi Raja
Department of Plant Morphology and Algology, Madurai Kamaraj University, Madurai, Tamil Nadu–620 021, India
Kasthuri Rajendran
Department of Plant Morphology and Algology, Madurai Kamaraj University, Madurai, Tamil Nadu–620 021, India
Manoharan Kumariah
Department of Plant Morphology and Algology, Madurai Kamaraj University, Madurai, Tamil Nadu–620 021, India
Sripriya Rajasekaran
Department of Biotechnology, Mepco Schlenk Engineering College, Sivakasi, Tamil Nadu‐ 626 005, India

Abstract


Plant lectins are the heterogenous group of glycoproteins extensively studied for their potent insecticidal property against Hemipteran pests. In this present study, the full‐length cDNA of monocot mannose‐binding insecticidal lectin gene was isolated from Allium ascalonicum leaves. The isolated Allium ascalonicum Lectin (AAL) gene was cloned in pGEM‐T vector, sequenced and the sequence was submitted to GenBank (KM096570.1). Sequence analysis revealed a 468 bp open reading frame (ORF) encoding a putative 155 amino acids agglutinin precursor. Multiple sequence alignment and phylogenetic analysis of AAL amino acid with those of 30 other Mannose binding lectin (MBL) sequences in NCBI revealed a high similarity of 85‐95% indicating that AAL is a member of the MBL super family and forms a cluster with other onion lectins. Secondary structure prediction and the homology modeling showed that AAL protein possess predominantly β‐sheets and three potential mannose‐binding motifs consisting of 5 amino acid residues QDNVY like other GNA lectins. The results of the insilico analysis predict that the Allium ascalonicum lectin gene can be another potent insecticidal protein.

Keywords


Hemipterans, Allium ascalonicum, Mannose Binding Lectins, In-Silico Analysis.

References