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Green Synthesis of Silver Nanoparticles Using Aqueous Extract of Taraxacum officinale and its Antimicrobial Activity
Aim of the present study is to focus on the biosynthesis of silver nanoparticles using Taraxacum officinale floral extract. The color of solution changes into dark brown indicating the formation of silver nanoparticles (AgNPs) upon addition of 1 mM silver nitrate. These nanoparticles were characterized by UV- Vis spectrophotometry which showed the absorption peak at 465 nm specific for AgNPs. Scanning electron microscopic image showed that the synthesized AgNPs were seemed to be spherical in morphology. Dynamic light Scattering (DLS)was used to determine the particles size of the nanoparticles and average diameter of AgNPs is found to be 545 nm ± 5 nm. Antibacterial activity of these green synthesized Ag- NPs was evaluated against selected pathogns such as Enterococcus faecalis and Pseudomonas aeruginosa by disc diffusion assay.These synthesized AgNPs showed a good antimicrobial activity against the selected bacterial pathogens.
Keywords
Taraxacum officinale, SEM, UV- vis Spectrophotometer.
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