South Indian Journal of Biological Sciences

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Optimization of Protease Production from Lysinibacillus fusiformis F3G5 Isolated from the Fish Gut of Oreochromis mossambicus


Affiliations
1 Department of Zoology, Lakshmipuram College of Arts and Science, Kanyakumari District, Tamilnadu, India
2 Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam, 629 502 Kanyakumari District, Tamil nadu, India
3 Department of Animal Science, Manonmaniam Sundaranar University, Tirunelveli, 627 012 Tamil nadu, India
 

Protease producing bacterium was isolated from the fish gut of Oreochromis mossambicus and identified as Lysinibacillus fusiformis F3G5 by 16S rRNA gene sequencing. The nutritional factors such as carbon sources, nitrogen sources, ionic sources and also environmental parameters such as temperature, pH, sodium chloride were optimized to enhance the yield of protease. Among the carbon sources, fructose (439.27±15.14 U/ml) enhanced enzyme production. Ammonium sulphate (439.27±15.14 U/ml) was found to be the best nitrogen source and Zn2+ ion (662.70±23.32 U/ml) significantly influenced on enzyme production. Protease production was found to be high at pH 7.0, 40 °C at 0.5% NaCl and the partially purified enzyme was highly active under these environmental conditions. The partially purified enzyme showed more than 60% activity in the presence of a few detergents. The demonstrated detergent stability of protease from L. fusiformis F3G5 infers that the enzyme has good compatibility with ingredients present in detergent formulation.

Keywords

Lysinibacillus fusiformis, Oreochromis mossambicus, Proteas.
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  • Optimization of Protease Production from Lysinibacillus fusiformis F3G5 Isolated from the Fish Gut of Oreochromis mossambicus

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Authors

Ponnumuthu Nandhakumari
Department of Zoology, Lakshmipuram College of Arts and Science, Kanyakumari District, Tamilnadu, India
Grasian Immanuel
Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam, 629 502 Kanyakumari District, Tamil nadu, India
Arunachalam Palavesam
Department of Animal Science, Manonmaniam Sundaranar University, Tirunelveli, 627 012 Tamil nadu, India

Abstract


Protease producing bacterium was isolated from the fish gut of Oreochromis mossambicus and identified as Lysinibacillus fusiformis F3G5 by 16S rRNA gene sequencing. The nutritional factors such as carbon sources, nitrogen sources, ionic sources and also environmental parameters such as temperature, pH, sodium chloride were optimized to enhance the yield of protease. Among the carbon sources, fructose (439.27±15.14 U/ml) enhanced enzyme production. Ammonium sulphate (439.27±15.14 U/ml) was found to be the best nitrogen source and Zn2+ ion (662.70±23.32 U/ml) significantly influenced on enzyme production. Protease production was found to be high at pH 7.0, 40 °C at 0.5% NaCl and the partially purified enzyme was highly active under these environmental conditions. The partially purified enzyme showed more than 60% activity in the presence of a few detergents. The demonstrated detergent stability of protease from L. fusiformis F3G5 infers that the enzyme has good compatibility with ingredients present in detergent formulation.

Keywords


Lysinibacillus fusiformis, Oreochromis mossambicus, Proteas.

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DOI: https://doi.org/10.22205/sijbs%2F2016%2Fv2%2Fi4%2F103448