South Indian Journal of Biological Sciences

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Purification, Characterization of a Novel Fibrinolytic Enzyme from Paenibacillus sp. IND8, and its in Vitro Thrombolytic Activity


Affiliations
1 International Centre for Nanobiotechnology, Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam–629 502, Kanyakumari District, Tamil Nadu, India
2 Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia
 

In the present study, a fibrinolytic enzyme from Paenibacillus sp. IND8 was isolated and characterized. This enzyme was purified by 12.4-fold using ammonium sulphate precipitation, diethyl amino ethyl-cellulose, and casein–agarose column chromatography. The purified enzyme showed homogenous in terms of sodium dodecyl sulfate-polyacrylamide gel electrophoresis with a molecular weight of 44 kDa. This enzyme depicted an optimal pH of 7.0 and was stable at pH 7.0–9.0. The optimal temperature was found to be 40°C and was highly stable at this temperature. The enzyme activity was inhibited by the addition of tested ions. The fibrinolytic enzyme dissolved fibrin net of blood clot completely in vitro, which may have potent application in the treatment of myocardial infarction.

Keywords

Paenibacillus sp., Fibrinolyic Enzyme, Fibrinolysis, Cardiovascular Diseases.
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  • Purification, Characterization of a Novel Fibrinolytic Enzyme from Paenibacillus sp. IND8, and its in Vitro Thrombolytic Activity

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Authors

Ponnuswamy Vijayaraghavan
International Centre for Nanobiotechnology, Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam–629 502, Kanyakumari District, Tamil Nadu, India
Samuel Gnana Prakash Vincent
International Centre for Nanobiotechnology, Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam–629 502, Kanyakumari District, Tamil Nadu, India
Mariadhas Valan Arasu
Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia

Abstract


In the present study, a fibrinolytic enzyme from Paenibacillus sp. IND8 was isolated and characterized. This enzyme was purified by 12.4-fold using ammonium sulphate precipitation, diethyl amino ethyl-cellulose, and casein–agarose column chromatography. The purified enzyme showed homogenous in terms of sodium dodecyl sulfate-polyacrylamide gel electrophoresis with a molecular weight of 44 kDa. This enzyme depicted an optimal pH of 7.0 and was stable at pH 7.0–9.0. The optimal temperature was found to be 40°C and was highly stable at this temperature. The enzyme activity was inhibited by the addition of tested ions. The fibrinolytic enzyme dissolved fibrin net of blood clot completely in vitro, which may have potent application in the treatment of myocardial infarction.

Keywords


Paenibacillus sp., Fibrinolyic Enzyme, Fibrinolysis, Cardiovascular Diseases.

References





DOI: https://doi.org/10.22205/sijbs%2F2016%2Fv2%2Fi4%2F103450