South Indian Journal of Biological Sciences

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Inhibitory Effect of Silver Nanoparticles against Pathogenic Vibrio anguillarum for the Treatment of Vibriosis


Affiliations
1 Aquaculture Biotechnology Laboratory, School of Biosciences and Technology, VIT University, Vellore – 632 014, Tamilnadu, India
2 Department of Biotechnology, Periyar University, Salem – 636 011, Tamilnadu, India
3 Centre for Ocean Research, Satyabama University, Chennai, Tamilnadu, India
 

In this study we have validated the bactericidal ability of silver nanoparticles (Ag NPs) bacterial infections in humans to restrain Vibriosis caused by the fish pathogen Vibrio anguillarum. The nanoparticles were synthesized chemically and characterized by UV-visible spectroscopy (UV-vis), scanning electron microscopy (SEM), Fourier transform infra-red spectroscopy (FT-IR) and Atomic Force Microscopy (AFM). UV-vis analysis proved the wavelength of the sample to be 418- 420nm, resembling the surface resonance peak specific for Ag NPs. SEM results revealed that the particles were in the size range of 20nm. The results indicated that Ag NPs possessed antibacterial activity against fish pathogen V. anguillarum. Destruction of bacterial cell was observed in Transmission electron microscopy (TEM) between different time periods after bacterial and nanoparticle interaction. Thus Ag NPs synthesized chemically can be effectively used as an antibacterial agent in treating fish diseases.

Keywords

Vibrio anguillarum, Fish Disease, Silver Nanoparticles, Antimicrobial Agent, TEM.
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  • Augustine R, Rajarathinam K. (2012). Synthesis and characterization of silver nanoparticles and its immobilization on alginate coated sutures for the prevention of surgical wound infections and the in vitro release studies. International Journal of Nano Dimension, 2(3),205-212.

  • Baticados M, Lavilla-Pitogo C, Cruz-Lacierda E, De La Pena L, Sunaz N. (1990). Studies on the chemical control of luminous bacteria Vibrio harveyi and V. splendidus isolated from diseased Penaeus monodon larvae and rearing water. Diseases of Aquatic Organisms, 9(2),133-139.

  • Berger T, Spadaro J, Chapin S, Becker R.(1976). Electrically generated silver ions: quantitative effects on bacterial and mammalian cells. Antimicrobial Agents and Chemotherapy, 9(2),357.

  • Brown T, Smith D. (1976). Effects of silver nitrate on the growth and ultrastructure of the yeast Cryptococcus albidus. Microbios letters .

  • DiSalvo L, Blecka J, Zebal R. (1978). Vibrio anguillarum and larval mortality in a California coastal shellfish hatchery. Applied and Environmental Microbiology, 35(1),219-221.

  • FAO, 2007. Fishery Information, Data and Statistics Unit (FIDI) Fishery Statistical Collections, Rome.

  • Fisheries F: Aquaculture Department (2009) The state of world fisheries and aquaculture 2008. Food and agriculture organization of the United Nations, Rome.

  • Le A-T, Le TT, Tran HH, Dang DA, Tran QH, Vu DL. (2012). Powerful colloidal silver nanoparticles for the prevention of gastrointestinal bacterial infections. Advances in Natural Sciences: Nanoscience and Nanotechnology, 3(4),045007.

  • Lee P, Meisel D. (1982). Adsorption and surface-enhanced Raman of dyes on silver and gold sols. The Journal of Physical Chemistry, 86(17),3391-3395.

  • Malaikannan L, Marimuthu PN, Ramar M, Baskaralingam V. (2012). Antibacterial Effect of Green Synthesized Silver Nanoparticles against Vibrio Sp. Isolated from Broiler Chicken'. Journal of Advanced Scientific Research, 3(4),51-54.

  • McDermott P, Zhao S, Wagner D, Simjee S, Walker R, White D. (2002). The food safety perspective of antibiotic resistance. Animal biotechnology, 13(1),71-84.

  • Ravikumar S, Gokulakrishnan R, Raj JA: Nanoparticles as a source for the treatment of fish diseases. (2012). Asian Pacific Journal of Tropical Disease, 2,S703-S706.

  • Richards R, Odelola H, Anderson B. (1983). Effect of silver on whole cells and spheroplasts of a silver resistant Pseudomonas aeruginosa. Microbios, 39,151-157.

  • Schreurs W, Rosenberg H. (1982). Effect of silver ions on transport and retention of phosphate by Escherichia coli. Journal of Bacteriology, 152(1),7-13.

  • Singh AK, Talat M, Singh DP, Srivastava O. (2010). Biosynthesis of gold and silver nanoparticles by natural precursor clove and their functionalization with amine group. Journal of Nanoparticle Research, 12(5),1667-1675.

  • Slawson RM, Van Dyke MI, Lee H, Trevors JT. (1992). Germanium and silver resistance, accumulation, and toxicity in microorganisms. Plasmid, 27(1),72-79.

  • Spadaro J, Berger T, Barranco S, Chapin S, Becker R. (1974). Antibacterial effects of silver electrodes with weak direct current. Antimicrobial agents and chemotherapy, 6(5),637-642.

  • Sujeewa A, Sani NA, Laina M. (2009). Prevalence of toxic genes of Vibrio parahaemolyticus in shrimps (Penaeus monodon) and culture environment. International Food Research Journal, 16(1).

  • Tollefson L, Miller MA. (2000). Antibiotic use in food animals: controlling the human health impact. Journal of AOAC international, 83(2):245-254.

  • Vandenberghe J. (2003). Climate forcing of fluvial system development: an evolution of ideas. Quaternary Science Reviews, 22(20):2053-2060.

  • Yakabe Y, Sano T, Ushio H, Yasunaga T. (1980). Kinetic studies of the interaction between silver ion and deoxyribonucleic acid. Chemistry Letters, (4):373-376.

  • Zavriev S, Minchenkova L, Vorličková M, Kolchinsky A, Volkenstein M, Ivanov V.(1979). Circular dichroism anisotropy of DNA with different modifications at N7 of guanine. Biochimica et Biophysica Acta (BBA)-Nucleic Acids and Protein Synthesis, 564(2),212-224.

  • Zhao G, Stevens Jr SE. (1998). Multiple parameters for the comprehensive evaluation of the susceptibility of Escherichia coli to the silver ion. Biometals, 11(1),27-32.


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  • Inhibitory Effect of Silver Nanoparticles against Pathogenic Vibrio anguillarum for the Treatment of Vibriosis

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Authors

M. Roohi Fatima
Aquaculture Biotechnology Laboratory, School of Biosciences and Technology, VIT University, Vellore – 632 014, Tamilnadu, India
S. Dinesh
Aquaculture Biotechnology Laboratory, School of Biosciences and Technology, VIT University, Vellore – 632 014, Tamilnadu, India
R. Ram kumar
Department of Biotechnology, Periyar University, Salem – 636 011, Tamilnadu, India
T. Perumal
Department of Biotechnology, Periyar University, Salem – 636 011, Tamilnadu, India
M. Ravi
Centre for Ocean Research, Satyabama University, Chennai, Tamilnadu, India
Ruchira Chatterjii
Aquaculture Biotechnology Laboratory, School of Biosciences and Technology, VIT University, Vellore – 632 014, Tamilnadu, India
R. Sudhakaran
Aquaculture Biotechnology Laboratory, School of Biosciences and Technology, VIT University, Vellore – 632 014, Tamilnadu, India

Abstract


In this study we have validated the bactericidal ability of silver nanoparticles (Ag NPs) bacterial infections in humans to restrain Vibriosis caused by the fish pathogen Vibrio anguillarum. The nanoparticles were synthesized chemically and characterized by UV-visible spectroscopy (UV-vis), scanning electron microscopy (SEM), Fourier transform infra-red spectroscopy (FT-IR) and Atomic Force Microscopy (AFM). UV-vis analysis proved the wavelength of the sample to be 418- 420nm, resembling the surface resonance peak specific for Ag NPs. SEM results revealed that the particles were in the size range of 20nm. The results indicated that Ag NPs possessed antibacterial activity against fish pathogen V. anguillarum. Destruction of bacterial cell was observed in Transmission electron microscopy (TEM) between different time periods after bacterial and nanoparticle interaction. Thus Ag NPs synthesized chemically can be effectively used as an antibacterial agent in treating fish diseases.

Keywords


Vibrio anguillarum, Fish Disease, Silver Nanoparticles, Antimicrobial Agent, TEM.

References





DOI: https://doi.org/10.22205/sijbs%2F2016%2Fv2%2Fi4%2F103452