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Immanuel, Grasian
- Optimization of Protease Production from Lysinibacillus fusiformis F3G5 Isolated from the Fish Gut of Oreochromis mossambicus
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Authors
Affiliations
1 Department of Zoology, Lakshmipuram College of Arts and Science, Kanyakumari District, Tamilnadu, IN
2 Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam, 629 502 Kanyakumari District, Tamil nadu, IN
3 Department of Animal Science, Manonmaniam Sundaranar University, Tirunelveli, 627 012 Tamil nadu, IN
1 Department of Zoology, Lakshmipuram College of Arts and Science, Kanyakumari District, Tamilnadu, IN
2 Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam, 629 502 Kanyakumari District, Tamil nadu, IN
3 Department of Animal Science, Manonmaniam Sundaranar University, Tirunelveli, 627 012 Tamil nadu, IN
Source
South Indian Journal of Biological Sciences, Vol 2, No 4 (2016), Pagination: 424-433Abstract
Protease producing bacterium was isolated from the fish gut of Oreochromis mossambicus and identified as Lysinibacillus fusiformis F3G5 by 16S rRNA gene sequencing. The nutritional factors such as carbon sources, nitrogen sources, ionic sources and also environmental parameters such as temperature, pH, sodium chloride were optimized to enhance the yield of protease. Among the carbon sources, fructose (439.27±15.14 U/ml) enhanced enzyme production. Ammonium sulphate (439.27±15.14 U/ml) was found to be the best nitrogen source and Zn2+ ion (662.70±23.32 U/ml) significantly influenced on enzyme production. Protease production was found to be high at pH 7.0, 40 °C at 0.5% NaCl and the partially purified enzyme was highly active under these environmental conditions. The partially purified enzyme showed more than 60% activity in the presence of a few detergents. The demonstrated detergent stability of protease from L. fusiformis F3G5 infers that the enzyme has good compatibility with ingredients present in detergent formulation.Keywords
Lysinibacillus fusiformis, Oreochromis mossambicus, Proteas.Full Text
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