South Indian Journal of Biological Sciences

Mohammed Musthafa ¹, Alwarrappan Sankaranarayanan ², Karuppiah Ponmurugan ², Suresh S. S. Raja ³

Affiliations
1 Department of Microbiology and Immunology, College of Medicine, Prince Sattam Bin Abdulaziz University, Al Kharj– 11942, SA
2 Department of Microbiology, K.S.Rangasamy College of Arts and Science, Tiruchengode – 637 215, Tamil Nadu, IN
3 Department of Microbiology, Bharathidasan University College, Perambalur, Tamil Nadu, IN

Abstract

The principal aim of the present investigation was the isolation, screening and testing of the antibacterial activity of the Loligo duvauceli extract derived from antimicrobial peptides (AMPs). A live marine animal specimen of L. duvaucelli (Indian squid) was collected from the trawling net of fishermen at Puthiyappa fishing harbor, Calicut, South West Coast of India. Ninty five percentages of methanol and ethanol were used to prepare the crude extract of marine invertebrate. Using the disc diffusion method, the antibacterial activity against the selected bacterial pathogens isolated from pus infection was studied. The antibacterial properties containing peptides were subjected to High Performance Thin Layer Chromatography (HPTLC) and partially purified. The HPTLC analysis of crude extracts ensured the presence of six different AMPs. Further, hemolytic assay, proteinase K treatment and heat treatment were undertaken to confirm the presence of proteins in the partially purified extracts (AMPs). The crude extract of L. duvaucelli showed antibacterial properties against all the tested clinical pathogens. The AMPs isolated from marine animals possessed antimicrobial properties. The HPTLC analysis of extracts revealed the presence of six different bioactive peptides and two AMPs among them exhibited effective antibacterial activities.

Keywords

Loligo duvauceli, Indian Squid, Antimicrobial Peptides, Pyogenic Infection.

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Hariharan Harikrishnan ¹, Shine Kadaikunnan ², Innasimuthu Ganesh Moorthy ³, Alexander Ronaldo Anuf ³, Karuppiah Ponmurugan ⁴, Rajaram Shyam Kumar ³

Affiliations
1 Department of Biotechnology Engineering, MET’S School of Engineering College, Mala, Thrissur, Kerala–680 735, IN
2 Department of Botany and Microbiology, College of Science, King Saud University, Riyadh - 11541, SA
3 Department of Biotechnology, Kamaraj College of Engineering and Technology, Virudhunagar–626 001, IN
4 Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, SA

Abstract

As like humans having the ability to precipitate minerals in the form of bones and teeth continuously, even some bacteria like Bacillus pasteurii and Bacillus sphaericus can continuously precipitate calcite, called as microbiological induced calcite precipitation. The present investigation discusses on the effects of B. pasteurii and B. sphaericus on durability of concrete beams. The efficacy of the bio deposition treatment on concrete beams with bacteria, alkali, sulfate and freeze-thaw environments along with water absorption was studied. The presence of a layer of calcium carbonate and microbial biomass resulted in a decrease of permeation properties of cementation materials. This technique not only improved the concrete strength but also enhanced water impermeability through calcite precipitation, quantified by X-ray diffraction analysis. Hence this biological treatment is highly desirable and eco-friendly. The present work guarantees a biological metabolic outcome with improvement in concrete and building material technology.

Keywords

Bacillus pasteurii, Concrete, Durability, Calcite Precipitation.

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